Pretransplant CD28 Biomarker (Levels of Expression and Quantification of Molecules per Cell) in Peripheral CD4+ T Cells Predicts Acute Rejection Episodes in Liver and Kidney Recipients

2016 ◽  
Vol 48 (9) ◽  
pp. 2987-2989 ◽  
Author(s):  
F. Boix ◽  
J.M. Bolarín ◽  
J. Eguía ◽  
G. Gonzalez-Martinez ◽  
J. De La Peña ◽  
...  
Keyword(s):  
T Cells ◽  
Acute Rejection ◽  
Cd4 T Cells ◽  
Kidney Recipients ◽  
Liver And Kidney

scholarly journals A Role for Human Renal Tubular Epithelial Cells in Direct Allo-Recognition by CD4+ T-Cells and the Effect of Ischemia-Reperfusion

2021 ◽  
Vol 22 (4) ◽  
pp. 1733
Author(s):  
Theodoros Eleftheriadis ◽  
Georgios Pissas ◽  
Marta Crespo ◽  
Evdokia Nikolaou ◽  
Vassilios Liakopoulos ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Acute Rejection ◽  
Cd4 T Cells ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Tubular Epithelial Cells ◽  
Renal Tubular Epithelial Cells ◽  
Hla Dr ◽  
Renal Tubular

Direct allorecognition is the earliest and most potent immune response against a kidney allograft. Currently, it is thought that passenger donor professional antigen-presenting cells (APCs) are responsible. Further, many studies support that graft ischemia-reperfusion injury increases the probability of acute rejection. We evaluated the possible role of primary human proximal renal tubular epithelial cells (RPTECs) in direct allorecognition by CD4+ T-cells and the effect of anoxia-reoxygenation. In cell culture, we detected that RPTECs express all the required molecules for CD4+ T-cell activation (HLA-DR, CD80, and ICAM-1). Anoxia-reoxygenation decreased HLA-DR and CD80 but increased ICAM-1. Following this, RPTECs were co-cultured with alloreactive CD4+ T-cells. In T-cells, zeta chain phosphorylation and c-Myc increased, indicating activation of T-cell receptor and co-stimulation signal transduction pathways, respectively. T-cell proliferation assessed with bromodeoxyuridine assay and with the marker Ki-67 increased. Previous culture of RPTECs under anoxia raised all the above parameters in T-cells. FOXP3 remained unaffected in all cases, signifying that proliferating T-cells were not differentiated towards a regulatory phenotype. Our results support that direct allorecognition may be mediated by RPTECs even in the absence of donor-derived professional APCs. Also, ischemia-reperfusion injury of the graft may enhance the above capacity of RPTECs, increasing the possibility of acute rejection.

Role of Regulated Upon Activation Normal T-Cell Expressed and Secreted in a Model of Retransplantation Acute Rejection Mediated by Alloreactive Memory CD4+ T Cells

2013 ◽  
Vol 45 (2) ◽  
pp. 546-551 ◽  
Author(s):  
X. Zhou ◽  
Z. Shan ◽  
H. Liang ◽  
Z. Lin ◽  
S. Qiu ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Acute Rejection ◽  
Cd4 T Cells ◽  
Memory Cd4 T Cells

High proportion of CD95+ and CD38+ in cultured CD8+ T cells predicts acute rejection and infection, respectively, in kidney recipients

2016 ◽  
Vol 34 ◽  
pp. 33-41 ◽  
Author(s):  
Esther Mancebo ◽  
María José Castro ◽  
Luís M. Allende ◽  
Paloma Talayero ◽  
Mercè Brunet ◽  
...  
Keyword(s):  
T Cells ◽  
Acute Rejection ◽  
Cd8 T Cells ◽  
Kidney Recipients

CP-690,550 INHIBITS INTERLEUKIN2-MEDIATED STAT5 ACTIVATION IN CD4+ T CELLS OF ADULT KIDNEY RECIPIENTS

2010 ◽  
Vol 90 ◽  
pp. 202
Author(s):  
R. Silva ◽  
K. Piard-Ruster ◽  
S. M. Krams ◽  
O. M. Martinez ◽  
S. Busque
Keyword(s):  
T Cells ◽  
Cd4 T Cells ◽  
Kidney Recipients ◽  
Adult Kidney

scholarly journals Multiplex immunofluorescence of spatial distribution of infiltrating T cells in different regions of hepatic lobules during liver transplantation rejection

2021 ◽  
Author(s):  
Shipeng Li ◽  
Guangpeng Zhou ◽  
Jie Sun ◽  
Bin Cui ◽  
Haiming Zhang ◽  
...  
Keyword(s):  
Liver Transplantation ◽  
T Cells ◽  
Spatial Distribution ◽  
Regulatory T Cells ◽  
Acute Rejection ◽  
Cd8 T Cells ◽  
Immune Cells ◽  
Cd4 T Cells ◽  
Rejection Level ◽  
Transplantation Rejection

Abstract It is still unclear whether there are differences in the types and functional status of immune cells in different areas of the liver lobules after liver transplantation rejection. The composition of infiltrating T cells in liver allografts during liver transplantation rejection is unclear and difficult to visualize on the same biopsy slide. We used multiplex immunofluorescence assays to study the spatial distribution of various types of infiltrating T cells in different areas of the liver lobules after liver transplantation. In the same area of the hepatic lobules, the percentage of CD4 + T cells, CD8 + T cells and regulatory T cells (Tregs) in the acute rejection group was higher than that in the nonacute rejection and normal groups. Within all three groups, the percentage of CD4 + T cells, CD8 + T cells and Tregs from the periportal to perivenous zones was increased first and then decreased. The percentage of CD8 + T cells increased gradually from the periportal to perivenous zones, the percentage of CD8 + T cells in perivenous zone was higher than in the transitional and periportal zones in the rejection group. In conclusion, the percentage of CD8 + T cells in different regions of liver lobules is closely related to rejection level after liver transplantation. Acute liver transplantation rejection may occur when the percentage of CD8 + T cells in the perivenous zone increases. Although the percentage of regional CD4 + T could not reflect the rejection level, but the number of CD4 + and CD8 + T cells in different regions was closely related to the rejection level.

BLOOD CD25+CD4+ T CELLS PHENOTYPE AND REGULATION ASSOCIATED MOLECULES IN “OPERATIONALLY TOLERANT” VERSUS CHRONIC REJECTION HUMAN KIDNEY RECIPIENTS.

2004 ◽  
Vol 78 ◽  
pp. 137
Author(s):  
C Braudeau ◽  
S Louis ◽  
A Dupont ◽  
M Giral ◽  
J P. Soulillou ◽  
...  
Keyword(s):  
T Cells ◽  
Cd4 T Cells ◽  
Chronic Rejection ◽  
Human Kidney ◽  
Kidney Recipients

scholarly journals In vitro intracellular IFNγ, IL-17 and IL-10 producing T cells correlates with the occurrence of post-transplant opportunistic infection in liver and kidney recipients

2018 ◽  
Vol 8 (1) ◽  
pp. 23-37
Author(s):  
Francisco Boix ◽  
Santiago Llorente ◽  
Jorge Eguía ◽  
Gema Gonzalez-Martinez ◽  
Rafael Alfaro ◽  
...  
Keyword(s):  
T Cells ◽  
Opportunistic Infection ◽  
Post Transplant ◽  
Kidney Recipients ◽  
Liver And Kidney

Activated CD4+ T cells enriched from spleen or colon induce apoptosis in intestinal epithellal cells via fas/fas ligand interactions

2001 ◽  
Vol 120 (5) ◽  
pp. A192-A192
Author(s):  
H TAKAISHI ◽  
T DENNING ◽  
K ITO ◽  
R MIFFLIN ◽  
P ERNST
Keyword(s):  
T Cells ◽  
Cd4 T Cells ◽  
Fas Ligand ◽  
Ligand Interactions
Sign in / Sign up

Export Citation Format

Share Document