Preliminary Clinical Data and the Comparison of the Safety and Efficacy of Autogenous Bone Grafts Versus Xenograft Implantations in Vertical Bone Deficiencies Before Dental Implant Installation

Abstract Several techniques have been proposed for vertical bone regeneration, and many of them use bone autogenous and allogeneic grafts. The purpose of this study was to compare demineralised freeze-dried bone allografts (DFDBA), fresh-frozen (FF) allografts, autogenous bone grafts to find differences between volumetric and histological quantity of bone formation and vertical bone growth dynamic. A vertical tissue regeneration bone model was performed in rabbit calvarias under general anaesthesia. Four hollow cylinders of pure titanium were screwed onto external cortical bone calvarias in eight rabbits. Each one of the cylinders was randomly filled with one intervention: DFDBA, FF, autogenous bone, or left to be filled with blood clot (BC) as control. Allogeneic grafts were obtained from a ninth animal following international standardised protocols for the harvesting, processing, and cryopreservation of allografts. Autogenous graft was obtained from the host femur scraping before adapting hollow cylinders. Animals were euthanized at 13 weeks. Vertical volume was calculated after probe device measurements of the new formed tissue inside the cylinders and after titanium cylinders were removed. Histomorphometry and fluorochrome staining were used to analyse quantity and dynamic of bone formation, respectively. Results showed that DFDBA and fresh-frozen bone improved the velocity and the quantity of bone deposition in distant portions of the basal plane of grafting. Remaining material in allograft groups was more intense than in autogenous group. Both allografts can be indicated as reliable alternatives for volume gain and vertical bone augmentation.

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Long-term results of mandibular reconstruction with autogenous bone grafts and oral implants after tumor resection

Clinical Oral Implants Research ◽

10.1111/j.1600-0501.2008.01542.x ◽

2008 ◽

Vol 19 (10) ◽

pp. 1074-1080 ◽

Cited By ~ 72

Author(s):

Matteo Chiapasco ◽

Giacomo Colletti ◽

Eugenio Romeo ◽

Marco Zaniboni ◽

Roberto Brusati

Keyword(s):

Tumor Resection ◽

Bone Grafts ◽

Mandibular Reconstruction ◽

Long Term Results ◽

Autogenous Bone ◽

Oral Implants ◽

Autogenous Bone Grafts

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Volume Changes of Iliac Crest Autogenous Bone Grafts After Vertical and Horizontal Alveolar Ridge Augmentation of Atrophic Maxillas and Mandibles: A 6-Year Computerized Tomographic Follow-Up

Journal of Oral and Maxillofacial Surgery ◽

10.1016/j.joms.2012.07.040 ◽

2012 ◽

Vol 70 (11) ◽

pp. 2559-2565 ◽

Cited By ~ 59

Author(s):

Carolina Sbordone ◽

Paolo Toti ◽

Franco Guidetti ◽

Luigi Califano ◽

Angela Santoro ◽

...

Keyword(s):

Iliac Crest ◽

Bone Grafts ◽

Autogenous Bone ◽

Alveolar Ridge ◽

Volume Changes ◽

Alveolar Ridge Augmentation ◽

Ridge Augmentation ◽

Autogenous Bone Grafts

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Osteogenic Potential of Autogenous Bone Grafts Harvested with Four Different Surgical Techniques

Journal of Dental Research ◽

10.1177/0022034511422718 ◽

2011 ◽

Vol 90 (12) ◽

pp. 1428-1433 ◽

Cited By ~ 61

Author(s):

R.J. Miron ◽

E. Hedbom ◽

N. Saulacic ◽

Y. Zhang ◽

A. Sculean ◽

...

Keyword(s):

Cell Attachment ◽

Surgical Techniques ◽

Bone Grafts ◽

Osteogenic Potential ◽

Autogenous Bone ◽

Mrna Levels ◽

Alizarin Red ◽

Osteogenic Response ◽

Autogenous Bone Grafts

The osteogenic potential of autogenous bone grafts is superior to that of allografts and xenografts because of their ability to release osteoinductive growth factors and provide a natural osteoconductive surface for cell attachment and growth. In this in vitro study, autogenous bone particles were harvested by four commonly used techniques and compared for their ability to promote an osteogenic response. Primary osteoblasts were isolated and seeded on autogenous bone grafts prepared from the mandibles of miniature pigs with a bone mill, piezo-surgery, bone scraper, and bone drill (bone slurry). The osteoblast cultures were compared for their ability to promote cell attachment, proliferation, and differentiation. After 4 and 8 hrs, significantly higher cell numbers were associated with bone mill and bone scraper samples compared with those acquired by bone slurry and piezo-surgery. Similar patterns were consistently observed up to 5 days. Furthermore, osteoblasts seeded on bone mill and scraper samples expressed significantly elevated mRNA levels of collagen, osteocalcin, and osterix at 3 and 14 days and produced more mineralized tissue as assessed by alizarin red staining. These results suggest that the larger bone graft particles produced by bone mill and bone scraper techniques have a higher osteogenic potential than bone slurry and piezo-surgery.

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