scholarly journals De novo assembly and analysis of midgut transcriptome of the argasid tick Ornithodoros erraticus and identification of genes differentially expressed after blood feeding

2018 ◽  
Vol 9 (6) ◽  
pp. 1537-1554 ◽  
Author(s):  
Ana Oleaga ◽  
Prosper Obolo-Mvoulouga ◽  
Raúl Manzano-Román ◽  
Ricardo Pérez-Sánchez
Keyword(s):  
De Novo Assembly ◽  
De Novo ◽  
Blood Feeding ◽  
Differentially Expressed ◽  
Argasid Tick

scholarly journals Detection of Two Highly Diverse Peribunyaviruses in Mosquitoes from Palenque, Mexico

Viruses ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 832 ◽  
Author(s):  
Anne Kopp ◽  
Alexandra Hübner ◽  
Florian Zirkel ◽  
Daniel Hobelsberger ◽  
Alejandro Estrada ◽  
...  
Keyword(s):  
De Novo Assembly ◽  
Mammalian Cells ◽  
New Genus ◽  
Tropical Rainforest ◽  
Virus Isolation ◽  
De Novo ◽  
Blood Feeding ◽  
Transmission Cycle ◽  
Mosquito Cells ◽  
Duck Species

The Peribunyaviridae family contains the genera Orthobunyavirus, Herbevirus, Pacuvirus, and Shangavirus. Orthobunyaviruses and pacuviruses are mainly transmitted by blood-feeding insects and infect a variety of vertebrates whereas herbeviruses and shangaviruses have a host range restricted to insects. Here, we tested mosquitoes from a tropical rainforest in Mexico for infections with peribunyaviruses. We identified and characterized two previously unknown viruses, designated Baakal virus (BKAV) and Lakamha virus (LAKV). Sequencing and de novo assembly of the entire BKAV and LAKV genomes revealed that BKAV is an orthobunyavirus and LAKV is likely to belong to a new genus. LAKV was almost equidistant to the established peribunyavirus genera and branched as a deep rooting solitary lineage basal to herbeviruses. Virus isolation attempts of LAKV failed. BKAV is most closely related to the bird-associated orthobunyaviruses Koongol virus and Gamboa virus. BKAV was successfully isolated in mosquito cells but did not replicate in common mammalian cells from various species and organs. Also cells derived from chicken were not susceptible. Interestingly, BKAV can infect cells derived from a duck species that is endemic in the region where the BKAV-positive mosquito was collected. These results suggest a narrow host specificity and maintenance in a mosquito–bird transmission cycle.

Differentially Expressed Genes in Hypericin-Containing Hypericum perforatum Leaf Tissues as Revealed by De Novo Assembly of RNA-Seq

2016 ◽  
Vol 34 (5) ◽  
pp. 1027-1041 ◽  
Author(s):  
Miroslav Soták ◽  
Odeta Czeranková ◽  
Daniel Klein ◽  
Katarína Nigutová ◽  
Lothar Altschmied ◽  
...  
Keyword(s):  
Differentially Expressed Genes ◽  
De Novo Assembly ◽  
Hypericum Perforatum ◽  
De Novo ◽  
Differentially Expressed ◽  
Rna Seq ◽  
Leaf Tissues

scholarly journals De novo assembly and annotation of the salivary gland transcriptome of Rhipicephalus appendiculatus male and female ticks during blood feeding

2016 ◽  
Vol 7 (4) ◽  
pp. 536-548 ◽  
Author(s):  
Minique H. de Castro ◽  
Daniel de Klerk ◽  
Ronel Pienaar ◽  
Abdalla A. Latif ◽  
D. Jasper G. Rees ◽  
...  
Keyword(s):  
Salivary Gland ◽  
De Novo Assembly ◽  
De Novo ◽  
Blood Feeding ◽  
Rhipicephalus Appendiculatus ◽  
Male And Female

scholarly journals De novo assembly of Chinese forest musk deer (Moschus berezovskii) transcriptome from Next-Generation mRNA Sequencing

2016 ◽  
Author(s):  
Zhongxian Xu ◽  
Hang Jie ◽  
Binlong Chen ◽  
Uma Gaur ◽  
Mingyao Yang ◽  
...  
Keyword(s):  
Differentially Expressed Genes ◽  
De Novo Assembly ◽  
De Novo ◽  
Functional Enrichment ◽  
Differentially Expressed ◽  
Musk Deer ◽  
Next Generation ◽  
Steroid Biosynthesis ◽  
Mrna Sequencing ◽  
Genetic Mechanisms

Musk secretion in male musk deer is regarded as a propitious mode of sexual election to attract a greater number of females. However, the genetic mechanisms of musk secretion are still poorly understood and unresolved making it necessary to elucidate the possible genetic mechanisms of musk formation. In the present study, we used heart and musk gland tissues from a male musk deer for next-generation mRNA sequencing, integrated with de novo assembly, unigenes annotation and differentially expressed genes analysis. A total of 239,383 transcripts and 208,730 unigenes were obtained from 2 pooled RNA samples. Annotated analysis indicated steroid compound metabolism (steroid biosynthesis, steroid hormone biosynthesis, aldosterone-regulated sodium reabsorption, terpenoid backbone biosynthesis) related to musk formation were annotated to many pathways; relevant genes were identified as well. In addition, 8,986 differentially expressed genes (6,068 up- and 2,198 down-regulated) between heart and musk gland were identified, among them, steroid component metabolism were abundant. Further exploration of functional enrichment analysis showed that pathways involved in musk secretion were up-regulated in musk gland compared with heart, especially steroid biosynthesis and terpenoid backbone biosynthesis whose metabolic productions were key components of musk. We identified several candidate genes such as DHCR7, DHCR24, NSDHL, CYP3A5, FDFT1, FDPS and HMGCL which were closely involved in metabolism of steroid, terpenoid and ketone bodies. Our data are expected to represent the most comprehensive sequence resource available for the forest musk deer so far, and provide a basis for further research on molecular genetics and functional genomics of musk secretion.

scholarly journals De novo assembly of Chinese forest musk deer (Moschus berezovskii) transcriptome from Next-Generation mRNA Sequencing

2016 ◽  
Author(s):  
Zhongxian Xu ◽  
Hang Jie ◽  
Binlong Chen ◽  
Uma Gaur ◽  
Mingyao Yang ◽  
...  
Keyword(s):  
Differentially Expressed Genes ◽  
De Novo Assembly ◽  
De Novo ◽  
Functional Enrichment ◽  
Differentially Expressed ◽  
Musk Deer ◽  
Next Generation ◽  
Steroid Biosynthesis ◽  
Mrna Sequencing ◽  
Genetic Mechanisms

Musk secretion in male musk deer is regarded as a propitious mode of sexual election to attract a greater number of females. However, the genetic mechanisms of musk secretion are still poorly understood and unresolved making it necessary to elucidate the possible genetic mechanisms of musk formation. In the present study, we used heart and musk gland tissues from a male musk deer for next-generation mRNA sequencing, integrated with de novo assembly, unigenes annotation and differentially expressed genes analysis. A total of 239,383 transcripts and 208,730 unigenes were obtained from 2 pooled RNA samples. Annotated analysis indicated steroid compound metabolism (steroid biosynthesis, steroid hormone biosynthesis, aldosterone-regulated sodium reabsorption, terpenoid backbone biosynthesis) related to musk formation were annotated to many pathways; relevant genes were identified as well. In addition, 8,986 differentially expressed genes (6,068 up- and 2,198 down-regulated) between heart and musk gland were identified, among them, steroid component metabolism were abundant. Further exploration of functional enrichment analysis showed that pathways involved in musk secretion were up-regulated in musk gland compared with heart, especially steroid biosynthesis and terpenoid backbone biosynthesis whose metabolic productions were key components of musk. We identified several candidate genes such as DHCR7, DHCR24, NSDHL, CYP3A5, FDFT1, FDPS and HMGCL which were closely involved in metabolism of steroid, terpenoid and ketone bodies. Our data are expected to represent the most comprehensive sequence resource available for the forest musk deer so far, and provide a basis for further research on molecular genetics and functional genomics of musk secretion.

De novo assembly and comparative transcriptome characterization of Poecilobdella javanica provide insight into blood feeding of medicinal leeches

2018 ◽  
Vol 14 (5) ◽  
pp. 352-361 ◽  
Author(s):  
Zichao Liu ◽  
Yanjie Wang ◽  
Xiangrong Tong ◽  
Yuan Su ◽  
Lijiang Yang ◽  
...  
Keyword(s):  
De Novo Assembly ◽  
De Novo ◽  
Blood Feeding ◽  
Comparative Transcriptome ◽  
Classic Model ◽  
Reconstructive Microsurgery ◽  
Medicinal Leeches ◽  
Insight Into

Leeches (family Hirudinidae) are classic model invertebrates used in diverse clinical treatments, such as reconstructive microsurgery, hypertension, and gangrene treatment.

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