Construction and characterization of human rotavirus recombinant VP8* subunit parenteral vaccine candidates

SUMMARYHuman rotavirus isolates from 1100 stool samples were analyzed by polyacrylamide gel electrophoresis, and 48 different migration patterns were detected. Heterogeneity in the migration of segment 10 was observed in both long and short electropherotypes in which three long and two short patterns were identified. In spite of these variations all short and long electropherotypes were subgrouped by enzyme immunoassay as subgroups I and II respectively. Mixed infections were detected in 17% of cases and the subgrouping correlated with the corresponding electropherotypes. The same electropherotypes were present in severe, mild and asymptomatic cases and no electropherotype was particularly associated with greater virulence. Furthermore, the electropherotypes isolated from nosocomial asymptomatic cases were the same as those detected from those admitted with severe diarrheoa. It seems unlikely that electropherotyping can be used to identify more virulent strains of rotavirus.

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Characterization of the NSP4 gene of group A human rotavirus G1P[8] strains circulating in Sapporo, Japan from 1987 to 2000

Journal of Medical Virology ◽

10.1002/jmv.23723 ◽

2013 ◽

Vol 86 (2) ◽

pp. 354-359 ◽

Cited By ~ 5

Author(s):

Masatoshi Tatsumi ◽

Yoshinobu Nagaoka ◽

Takeshi Tsugawa ◽

Yuko Yoto ◽

Tsukasa Hori ◽

...

Keyword(s):

Nsp4 Gene ◽

Human Rotavirus ◽

Group A

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Full genome characterization of human Rotavirus A strains isolated in Cameroon, 2010–2011: Diverse combinations of the G and P genes and lack of reassortment of the backbone genes

Infection Genetics and Evolution ◽

10.1016/j.meegid.2014.10.009 ◽

2014 ◽

Vol 28 ◽

pp. 537-560 ◽

Cited By ~ 23

Author(s):

Valentine Ngum Ndze ◽

Mathew Dioh Esona ◽

Eric Akum Achidi ◽

Kamga Hortense Gonsu ◽

Renáta Dóró ◽

...

Keyword(s):

Full Genome ◽

Genome Characterization ◽

Rotavirus A ◽

Human Rotavirus

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Molecular characterization of rotavirus strains detected during a clinical trial of a human rotavirus vaccine in Blantyre, Malawi

Vaccine ◽

10.1016/j.vaccine.2011.09.119 ◽

2012 ◽

Vol 30 ◽

pp. A140-A151 ◽

Cited By ~ 12

Author(s):

Toyoko Nakagomi ◽

Osamu Nakagomi ◽

Winifred Dove ◽

Yen Hai Doan ◽

Desiree Witte ◽

...

Keyword(s):

Clinical Trial ◽

Molecular Characterization ◽

Rotavirus Vaccine ◽

Human Rotavirus

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Molecular characterization of group A rotaviruses detected in children with gastroenteritis in Ireland in 2006–2009

Epidemiology and Infection ◽

10.1017/s0950268811000306 ◽

2011 ◽

Vol 140 (2) ◽

pp. 247-259 ◽

Cited By ~ 18

Author(s):

O. CASHMAN ◽

P. J. COLLINS ◽

G. LENNON ◽

B. CRYAN ◽

V. MARTELLA ◽

...

Keyword(s):

Sequence Analysis ◽

Irish Population ◽

Surveillance Activity ◽

Human Rotavirus ◽

Group A Rotaviruses ◽

Group A ◽

Hospital Acquired ◽

Rotavirus Infections ◽

Pcr Genotyping

SUMMARYCommunity and hospital-acquired cases of human rotavirus are responsible for millions of gastroenteritis cases in children worldwide, chiefly in developing countries, and vaccines are now available. During surveillance activity for human rotavirus infections in Ireland, between 2006 and 2009, a total of 420 rotavirus strains were collected and analysed. Upon either PCR genotyping and sequence analysis, a variety of VP7 (G1–G4 and G9) and VP4 (P[4], P[6], P[8] and P[9]) genotypes were detected. Strains G1P[8] were found to be predominant throughout the period 2006–2008, with slight fluctuations seen in the very limited samples available in 2008–2009. Upon either PCR genotyping and sequence analysis of selected strains, the G1, G3 and G9 viruses were found to contain E1 (Wa-like) NSP4 and I1 VP6 genotypes, while the analysed G2 strains possessed E2 NSP4 and I2 VP6 genotypes, a genetic make-up which is highly conserved in the major human rotavirus genogroups Wa- and Kun-like, respectively. Upon sequence analysis of the most common VP4 genotype, P[8], at least two distinct lineages were identified, both unrelated to P[8] Irish rotaviruses circulating in previous years, and more closely related to recent European humans rotaviruses. Moreover, sequence analysis of the VP7 of G1 rotaviruses revealed the onset of a G1 variant, previously unseen in the Irish population.

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Molecular-Genetic Characterization of Human Rotavirus A Strains Circulating in Moscow, Russia (2009–2014)

Virologica Sinica ◽

10.1007/s12250-018-0043-0 ◽

2018 ◽

Vol 33 (4) ◽

pp. 304-313 ◽

Cited By ~ 5

Author(s):

Victoria Kiseleva ◽

Evgeny Faizuloev ◽

Elena Meskina ◽

Anna Marova ◽

Alexey Oksanich ◽

...

Keyword(s):

Genetic Characterization ◽

Molecular Genetic ◽

Rotavirus A ◽

Human Rotavirus ◽

Molecular Genetic Characterization

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An Overview of the Progress Made on the Coronavirus Vaccine

E3S Web of Conferences ◽

10.1051/e3sconf/202018503042 ◽

2020 ◽

Vol 185 ◽

pp. 03042

Author(s):

Yu Fang

Keyword(s):

United States ◽

Infectious Diseases ◽

Incidence Rate ◽

Scientific Reasoning ◽

Vaccine Development ◽

The United States ◽

Ideal Solution ◽

Vaccine Candidates ◽

Number Of Individuals

The Coronavirus Disease-2019 (COVID-19) pandemic has led to a critical economic crash around the globe, affecting billions of people worldwide. Without a cure, the number of cases continues to increase exponentially. Countries, including the United States, Brazil, and India, currently lead in the number of cases with numbers soaring in the millions. Immunization is crucial to preventing the spread of infectious diseases and can help a large number of individuals quickly while keeping current cases under control. Following the publication of the genome sequence of SARS-CoV-2, vaccine development has been accelerated at an unprecedented rate. 115 vaccine candidates are currently under study with the hope of finding an ideal solution and mitigating the Coronavirus incidence rate. With some vaccine candidates having more potential than others, this review focuses on the characterization of different vaccine options. The analysis of probable vaccines, including mRNA vaccines and adenovirus vaccines, is conducted, and the scientific reasoning behind the vaccines is also discussed. In this review, the latest strategy vaccine is introduced and the effective vaccines are analysed.

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