Development of a candidate influenza vaccine based on virus-like particles displaying influenza M2e peptide into the immunodominant region of hepatitis B core antigen: Broad protective efficacy of particles carrying four copies of M2e

Vaccine ◽  
2015 ◽  
Vol 33 (29) ◽  
pp. 3398-3406 ◽  
Author(s):  
Liudmila M. Tsybalova ◽  
Liudmila A. Stepanova ◽  
Victor V. Kuprianov ◽  
Elena A. Blokhina ◽  
Marina V. Potapchuk ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Influenza Vaccine ◽  
Protective Efficacy ◽  
Core Antigen ◽  
Virus Like Particles ◽  
Immunodominant Region ◽  
Hepatitis B Core Antigen

Spatial Structure and Insertion Capacity of Immunodominant Region of Hepatitis B Core Antigen

Intervirology ◽  
1996 ◽  
Vol 39 (1-2) ◽  
pp. 16-22 ◽  
Author(s):  
Galina Borisova ◽  
Olga Borschukova Wanst ◽  
Guna Mezule ◽  
Dace Skrastina ◽  
Ivars Petrovskis ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Spatial Structure ◽  
Core Antigen ◽  
Immunodominant Region ◽  
Hepatitis B Core Antigen

scholarly journals Tandem Fusion of Hepatitis B Core Antigen Allows Assembly of Virus-Like Particles in Bacteria and Plants with Enhanced Capacity to Accommodate Foreign Proteins

PLoS ONE ◽  
2015 ◽  
Vol 10 (4) ◽  
pp. e0120751 ◽  
Author(s):  
Hadrien Peyret ◽  
Annick Gehin ◽  
Eva C. Thuenemann ◽  
Donatienne Blond ◽  
Aadil El Turabi ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Core Antigen ◽  
Virus Like Particles ◽  
Tandem Fusion ◽  
Foreign Proteins ◽  
Hepatitis B Core Antigen

scholarly journals Efficient Production of Chimeric Hepatitis B Virus-Like Particles Bearing an Epitope of Hepatitis E Virus Capsid by Transient Expression in Nicotiana benthamiana

Life ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 64
Author(s):  
Gergana Zahmanova ◽  
Milena Mazalovska ◽  
Katerina Takova ◽  
Valentina Toneva ◽  
Ivan Minkov ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Nicotiana Benthamiana ◽  
Hepatitis E Virus ◽  
Chimeric Protein ◽  
Hepatitis E ◽  
Core Antigen ◽  
Efficient Production ◽  
Virus Like Particles ◽  
B Virus

The core antigen of hepatitis B virus (HBcAg) is capable of self-assembly into virus-like particles (VLPs) when expressed in a number of heterologous systems. Such VLPs are potential carriers of foreign antigenic sequences for vaccine design. In this study, we evaluated the production of chimeric HBcAg VLPs presenting a foreign epitope on their surface, the 551–607 amino acids (aa) immunological epitope of the ORF2 capsid protein of hepatitis E virus. A chimeric construct was made by the insertion of 56 aa into the immunodominant loop of the HBcAg. The sequences encoding the chimera were inserted into the pEAQ-HT vector and infiltrated into Nicotiana benthamiana leaves. The plant-expressed chimeric HBcHEV ORF2 551–607 protein was recognized by an anti-HBcAg mAb and anti-HEV IgG positive swine serum. Electron microscopy showed that plant-produced chimeric protein spontaneously assembled into “knobbly” ~34 nm diameter VLPs. This study shows that HBcAg is a promising carrier platform for the neutralizing epitopes of hepatitis E virus (HEV) and the chimeric HBcAg/HEV VLPs could be a candidate for a bivalent vaccine.

scholarly journals The direct recovery of recombinant hepatitis B core antigen from disruptate derived from continuous-flow bead milling

2008 ◽  
Vol 50 (1) ◽  
pp. 49 ◽  
Author(s):  
Chin Woi Ho ◽  
Wen Siang Tan ◽  
Suryani Kamaruddin ◽  
Tau Chuan Ling ◽  
Beng Ti Tey
Keyword(s):  
Hepatitis B ◽  
Continuous Flow ◽  
Core Antigen ◽  
Recombinant Hepatitis ◽  
Bead Milling ◽  
Hepatitis B Core Antigen
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