The mouse induced Pluripotent Stem cell-derived cardiomyocytes: Detection of pro-arrhythmia, chronotropy, inotropy and cardiac cell death by RTCA cardio measurement

2014 ◽  
Vol 70 (3) ◽  
pp. 351-352 ◽  
Author(s):  
Aurore Colomar ◽  
Lucille Haen ◽  
Simon Loiodice ◽  
Gaelle Toussaint ◽  
Franck Atienzar
Keyword(s):  
Cell Death ◽  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Cardiac Cell ◽  
Induced Pluripotent

scholarly journals Contractile force measurement of human induced pluripotent stem cell-derived cardiac cell sheet-tissue

PLoS ONE ◽  
2018 ◽  
Vol 13 (5) ◽  
pp. e0198026 ◽  
Author(s):  
Daisuke Sasaki ◽  
Katsuhisa Matsuura ◽  
Hiroyoshi Seta ◽  
Yuji Haraguchi ◽  
Teruo Okano ◽  
...  
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Force Measurement ◽  
Cell Sheet ◽  
Induced Pluripotent Stem Cell ◽  
Contractile Force ◽  
Cardiac Cell ◽  
Induced Pluripotent

Role of Mcl-1 in regulation of cell death in human induced pluripotent stem cell-derived cardiomyocytes in vitro

2018 ◽  
Vol 360 ◽  
pp. 88-98 ◽  
Author(s):  
Liang Guo ◽  
Sandy Eldridge ◽  
Michael Furniss ◽  
Jodie Mussio ◽  
Myrtle Davis
Keyword(s):  
Cell Death ◽  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Induced Pluripotent

scholarly journals Mitochondrial dysfunction in iPSC-derived neurons of subjects with chronic mountain sickness

2018 ◽  
Vol 125 (3) ◽  
pp. 832-840 ◽  
Author(s):  
Helen Zhao ◽  
Guy Perkins ◽  
Hang Yao ◽  
David Callacondo ◽  
Otto Appenzeller ◽  
...  
Keyword(s):  
Cell Death ◽  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Mitochondrial Dynamics ◽  
Induced Pluripotent Stem Cell ◽  
Mitochondrial Morphology ◽  
Chronic Mountain Sickness ◽  
Atp Levels ◽  
Mountain Sickness ◽  
Induced Pluripotent

Patients with chronic mountain sickness (CMS) suffer from hypoxemia, erythrocytosis, and numerous neurologic deficits. Here we used induced pluripotent stem cell (iPSC)-derived neurons from both CMS and non-CMS subjects to study CMS neuropathology. Using transmission electron microscopy, we report that CMS neurons have a decreased mitochondrial volume density, length, and less cristae membrane surface area. Real-time PCR confirmed a decreased mitochondrial fusion gene optic atrophy 1 (OPA1) expression. Immunoblot analysis showed an accumulation of the short isoform of OPA1 (S-OPA1) in CMS neurons, which have reduced ATP levels under normoxia and increased lactate dehydrogenase (LDH) release and caspase 3 activation after hypoxia. Improving the balance between the long isoform of OPA1 and S-OPA1 in CMS neurons increased the ATP levels and attenuated LDH release under hypoxia. Our data provide initial evidence for altered mitochondrial morphology and function in CMS neurons, and reveal increased cell death under hypoxia due in part to altered mitochondrial dynamics. NEW & NOTEWORTHY Induced pluripotent stem cell-derived neurons from chronic mountain sickness (CMS) subjects have altered mitochondrial morphology and dynamics, and increased sensitivity to hypoxic stress. Modification of OPA1 can attenuate cell death after hypoxic treatment, providing evidence that altered mitochondrial dynamics play an important role in increased vulnerability under stress in CMS neurons.

Use of 3D culture systems to generate human induced pluripotent stem cell-derived [beta]-cells in vitro

2018 ◽  
Author(s):  
Fantuzzi Federica ◽  
Toivonen Sanna ◽  
Schiavo Andrea Alex ◽  
Pachera Nathalie ◽  
Rajaei Bahareh ◽  
...  
Keyword(s):  
Stem Cell ◽  
Beta Cells ◽  
Pluripotent Stem Cell ◽  
3D Culture ◽  
Induced Pluripotent Stem Cell ◽  
Culture Systems ◽  
Induced Pluripotent
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