Development of a real time reverse transcriptase polymerase chain reaction for the detection of bovine respiratory syncytial virus in clinical samples and its comparison with immunohistochemistry and immunofluorescence antibody testing

2008 ◽  
Vol 126 (1-3) ◽  
pp. 264-270 ◽  
Author(s):  
Kim Willoughby ◽  
Karen Thomson ◽  
Madeleine Maley ◽  
Janice Gilray ◽  
Sandra Scholes ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Respiratory Syncytial Virus ◽  
Reverse Transcriptase ◽  
Real Time ◽  
Clinical Samples ◽  
Antibody Testing ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Syncytial Virus ◽  
Immunofluorescence Antibody

One-step reverse transcriptase polymerase chain reaction for the diagnosis of respiratory syncytial virus in children

2008 ◽  
Vol 148 (1-2) ◽  
pp. 115-119 ◽  
Author(s):  
Cesar Augusto do Nascimento ◽  
Andréa Lima Leal ◽  
Thereza Silva Souza ◽  
Cláudia Trigo Pedroso de Moraes ◽  
Priscila Comone ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Respiratory Syncytial Virus ◽  
Reverse Transcriptase ◽  
Chain Reaction ◽  
One Step ◽  
Polymerase Chain ◽  
Syncytial Virus

Midturbinate Swabs Are Comparable to Nasopharyngeal Swabs for Quantitative Detection of Respiratory Syncytial Virus in Infants

2018 ◽  
Vol 8 (6) ◽  
pp. 554-558 ◽  
Author(s):  
Anne J Blaschke ◽  
Matt McKevitt ◽  
Krow Ampofo ◽  
Tammi Lewis ◽  
Hao Chai ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Respiratory Syncytial Virus ◽  
Real Time ◽  
Reverse Transcription ◽  
Quantitative Polymerase Chain Reaction ◽  
Quantitative Detection ◽  
Chain Reaction ◽  
Viral Loads ◽  
Polymerase Chain ◽  
Syncytial Virus

Abstract Nasopharyngeal (NP) swabs are generally used to detect respiratory syncytial virus (RSV) in infants. However, midturbinate (MT) swabs may provide comparable results. In this study, we enrolled hospitalized infants aged <24 months with RSV and collected NP and MT swabs. The resulting viral loads measured by real-time reverse-transcription quantitative polymerase chain reaction were similar. Most parents preferred MT swabs over NP swabs.

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