Serum antibody isotype responses of Fasciola-infected sheep and cattle to excretory and secretory products of Fasciola species

2006 ◽  
Vol 141 (3-4) ◽  
pp. 234-242 ◽  
Author(s):  
I.K. Phiri ◽  
A.M. Phiri ◽  
L.J.S. Harrison
Keyword(s):  
Serum Antibody ◽  
Infected Sheep ◽  
Antibody Isotype ◽  
Fasciola Species ◽  
Secretory Products

scholarly journals Surface Envelope Glycoprotein Is B-Lymphocyte Immunodominant in Sheep Naturally Infected with Ovine Progressive Pneumonia Virus

2005 ◽  
Vol 12 (6) ◽  
pp. 797-800 ◽  
Author(s):  
Lynn M. Herrmann ◽  
Travis C. McGuire ◽  
Isidro Hötzel ◽  
Gregory S. Lewis ◽  
Donald P. Knowles
Keyword(s):  
Envelope Glycoprotein ◽  
B Lymphocyte ◽  
Serum Antibody ◽  
Viral Proteins ◽  
Infected Sheep ◽  
Transmembrane Glycoprotein ◽  
Immunodominant Antigen ◽  
Surface Envelope Glycoprotein ◽  
Surface Envelope ◽  
Ovine Progressive Pneumonia

ABSTRACT The B-lymphocyte-immunodominant antigen involved in naturally ovine progressive pneumonia virus (OPPV)-infected mature sheep remains unknown. Therefore, the amount of antibody in sera from 10 naturally OPPV-infected sheep was evaluated by immunoprecipitation (IP) of the major viral proteins in [35S]methionine/cysteine-labeled OPPV (whole virus) lysate. Using an excess of OPPV proteins in whole-virus lysate, 8 out of 10 sheep had the highest serum antibody IP endpoint titers to the gp135 surface envelope glycoprotein (SU). Also, 2 out of 10 sheep had equivalent serum antibody IP endpoint titers to the transmembrane glycoprotein oligomer (TM90) and SU. Since these data indicate that SU is the immunodominant protein in most mature sheep persistently infected with OPPV, SU-specific diagnostic serological assays can be utilized for OPPV diagnosis.

Heterogeneity in the recognition of Ostertagia circumcincta antigens by serum antibody from mature, infected sheep

1997 ◽  
Vol 19 (5) ◽  
pp. 235-242 ◽  
Author(s):  
L. McCRIRIE ◽  
K. BAIRDEN ◽  
C. BRITTON ◽  
J. BUITKAMP ◽  
J.B. McKEAND ◽  
...  
Keyword(s):  
Serum Antibody ◽  
Infected Sheep

Serum antibody response and Cryptosporidium parvum oocyst antigens recognized by sera from naturally infected sheep

2002 ◽  
Vol 104 (3) ◽  
pp. 187-197 ◽  
Author(s):  
J. Quı́lez ◽  
C.A. Vergara-Castiblanco ◽  
M.E. Ares-Mazás ◽  
C. Sánchez-Acedo ◽  
E. del Cacho ◽  
...  
Keyword(s):  
Antibody Response ◽  
Cryptosporidium Parvum ◽  
Serum Antibody ◽  
Infected Sheep ◽  
Serum Antibody Response ◽  
Cryptosporidium Parvum Oocyst

scholarly journals Safety and Immunogenicity of a Proteosome-Shigella flexneri 2a Lipopolysaccharide Vaccine Administered Intranasally to Healthy Adults

2001 ◽  
Vol 69 (7) ◽  
pp. 4545-4553 ◽  
Author(s):  
Louis F. Fries ◽  
Andrew D. Montemarano ◽  
Corey P. Mallett ◽  
David N. Taylor ◽  
Thomas L. Hale ◽  
...  
Keyword(s):  
Protective Efficacy ◽  
Statistical Significance ◽  
Shigella Flexneri ◽  
Geometric Mean ◽  
Outer Membrane Proteins ◽  
Serum Antibody ◽  
Immunoglobulin A ◽  
Elevated Serum ◽  
Healthy Adults ◽  
Antibody Isotype

ABSTRACT We studied the safety and immunogenicity of a Shigella flexneri 2a vaccine comprising native S. flexneri 2a lipopolysaccharide (LPS) complexed to meningococcal outer membrane proteins—proteosomes—in normal, healthy adults. A two-dose series of immunizations was given by intranasal spray, and doses of 0.1, 0.4, 1.0, and 1.5 mg (based on protein) were studied in a dose-escalating design. The vaccine was generally well tolerated. The most common reactions included rhinorrhea and nasal stuffiness, which were clearly dose related (P ≤ 0.05). These reactions were self-limited and generally mild. The vaccine elicitedS. flexneri 2a LPS-specific immunoglobulin A (IgA), IgG, and IgM antibody-secreting cells (ASCs) in a dose-responsive manner. At doses of 1.0 or 1.5 mg, highly significant (P < 0.001) increases in ASCs of all antibody isotypes occurred and 95% of subjects had an ASC response in at least one antibody isotype. Dose-related serum antibody responses were observed, with geometric mean two- to fivefold rises in specific serum IgA and IgG titers and two- to threefold rises in IgM in the 1.0- and 1.5-mg-dose groups (P < 0.0001 for each isotype). Elevated serum antibody levels persisted through day 70. Increases in fecal IgG and IgA and also in urinary IgA specific for S. flexneri 2a LPS were demonstrated. These were most consistent and approached statistical significance (P = 0.02 to 0.12 for various measures) on day 70 after the first dose. The magnitude of immune responses to intranasally administered proteosome-S. flexneri 2a LPS vaccine is similar to those reported for live vaccine candidates associated with protective efficacy in human challenge models, and further evaluation of this product is warranted.

Characterization of the antibody isotype response in serum and milk of heifers vaccinated with a Staphylococcus aureus bacterin (LysiginTM)

2007 ◽  
Vol 74 (2) ◽  
pp. 239-246 ◽  
Author(s):  
Christopher D Luby ◽  
John R Middleton ◽  
Jianneng Ma ◽  
Carol L Rinehart ◽  
Scott Bucklin ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Capsular Polysaccharide ◽  
Serum Antibody ◽  
Test Sample ◽  
Negative Control ◽  
Late Gestation ◽  
Antibody Isotype ◽  
Staph Aureus ◽  
Dairy Heifers ◽  
Serotype 5

The objective was to characterize the antibody isotype responses to vaccination with a commercial Staphylococcus aureus bacterin in dairy heifers. Twenty-five Holstein-Friesian dairy heifers were assigned at random to one of two groups, vaccinates (n=14) or controls (n=11). Vaccinates received two 5-ml doses of Lysigin 28 d apart in late gestation. Both groups were challenged with a heterologous serotype 5 strain of Staph. aureus by aseptic intramammary infusion on days 6, 7 and 8 of lactation. Samples for serum antibody isotype (IgG, IgG1, IgG2 and IgM) determinations were taken before each vaccination, immediately prior to challenge (6 d post-calving) and at the end of the study (28 d post-calving). Samples for milk antibody isotype determinations were taken immediately prior to challenge and at the end of the study. Antibody isotype sample-to-positive ratios (S[ratio ]P ratio=(mean test sample OD−mean within-plate negative control OD)/(mean within-plate positive control OD−mean within-plate negative control OD)) were determined in milk and serum using a series of ELISAs coated with different strains of Staph. aureus belonging to capsular polysaccharide (CP) serotype 5 or 8 or surface polysaccharide (SP) serotype 336. Vaccinates had higher mean serum IgG1 and IgG2 S[ratio ]P ratios than controls against all three strains of Staph. aureus (P[les ]0·023). Vaccinates had higher mean milk IgG S[ratio ]P ratios than controls against CP8 and SP336 strains of Staph. aureus (P[les ]0·030). Hence, a humoural immune response to the vaccine was detected in serum and milk, but responses varied according to strain and antibody isotype tested.

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