Combination of cell culture and quantitative PCR (cc–qPCR) to assess disinfectants efficacy on Cryptosporidium oocysts under standardized conditions

Veterinary Parasitology ◽

10.1016/j.vetpar.2009.09.042 ◽

2010 ◽

Vol 167 (1) ◽

pp. 43-49 ◽

Author(s):

M. Shahiduzzaman ◽

V. Dyachenko ◽

J. Keidel ◽

R. Schmäschke ◽

A. Daugschies

Keyword(s):

Cell Culture ◽

Quantitative Pcr ◽

Cryptosporidium Oocysts

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Investigations of the relationship between use of in vitro cell culture-quantitative PCR and a mouse-based bioassay for evaluating critical factors affecting the disinfection performance of pulsed UV light for treating Cryptosporidium parvum oocysts in saline

Journal of Microbiological Methods ◽

10.1016/j.mimet.2010.01.017 ◽

2010 ◽

Vol 80 (3) ◽

pp. 267-273 ◽

Author(s):

Mary Garvey ◽

Hugh Farrell ◽

Martin Cormican ◽

Neil Rowan

Keyword(s):

Cell Culture ◽

Quantitative Pcr ◽

Cryptosporidium Parvum ◽

Uv Light ◽

Critical Factors ◽

Factors Affecting ◽

Pulsed Uv Light ◽

Cryptosporidium Parvum Oocysts ◽

The Relationship

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Cell Culture Infectivity to Assess Chlorine Disinfection of Cryptosporidium Oocysts in Water

Methods in Molecular Biology - Cryptosporidium ◽

10.1007/978-1-4939-9748-0_16 ◽

2019 ◽

pp. 283-302

Author(s):

Jennifer L. Murphy ◽

Michael J. Arrowood

Keyword(s):

Cell Culture ◽

Chlorine Disinfection ◽

Cryptosporidium Oocysts

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Applicability of integrated cell culture reverse transcriptase quantitative PCR (ICC-RTqPCR) for the simultaneous detection of the four human enteric enterovirus species in disinfection studies

Journal of Virological Methods ◽

10.1016/j.jviromet.2018.05.008 ◽

2018 ◽

Vol 258 ◽

pp. 35-40 ◽

Author(s):

Hodon Ryu ◽

Karen A. Schrantz ◽

Nichole E. Brinkman ◽

Laura A. Boczek

Keyword(s):

Cell Culture ◽

Reverse Transcriptase ◽

Quantitative Pcr ◽

Simultaneous Detection

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Retention of Rotavirus Infectivity in Mussels Heated by Using the French Recipe Moules Marinières

Journal of Food Protection ◽

10.4315/0362-028x.jfp-15-191 ◽

2015 ◽

Vol 78 (11) ◽

pp. 2064-2069 ◽

Author(s):

DORIS SOBRAL MARQUES SOUZA ◽

TAKAYUKI MIURA ◽

CÉCILE LE MENNEC ◽

CÉLIA REGINA MONTE BARARDI ◽

FRANÇOISE S. LE GUYADER

Keyword(s):

Cell Culture ◽

Real Time ◽

Quantitative Pcr ◽

Infectious Virus ◽

Real Time Quantitative Pcr ◽

Infectivity Assay ◽

To evaluate the persistence of infectious virus after heating, mussels contaminated with a rotavirus strain were prepared following the French recipe moules marinières (mariner's mussels). Rotavirus was then quantified by real-time quantitative PCR (RT-qPCR) and a cell culture infectivity assay. Results showed the persistence of infectious virus after 3 min of cooking. After 5 min, when no infectious virus could be detected, the RT-qPCR approach showed a 1-log decrease compared with concentrations detected after 1 min of cooking.

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Quantification of Matrix Protein mRNAs Expression During Mineralized Tissue Formation in Rat Marrow Cell Culture by a Real Time Quantitative PCR Method

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.192-195.367 ◽

2000 ◽

Vol 192-195 ◽

pp. 367-372

Author(s):

Y. Dohi ◽

Hideo Nakajima ◽

Hajime Ohgushi ◽

K. Yonemasu

Keyword(s):

Cell Culture ◽

Real Time ◽

Quantitative Pcr ◽

Matrix Protein ◽

Marrow Cell ◽

Tissue Formation ◽

Mineralized Tissue ◽

Real Time Quantitative Pcr ◽

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Diagnostic value of quantitative PCR for adenovirus detection in stool samples as compared with antigen detection and cell culture in haematopoietic stem cell transplant recipients

Clinical Microbiology and Infection ◽

10.1111/j.1469-0691.2011.03488.x ◽

2011 ◽

Vol 17 (11) ◽

pp. 1674-1680 ◽

Author(s):

H. Jeulin ◽

A. Salmon ◽

P. Bordigoni ◽

V. Venard

Keyword(s):

Cell Culture ◽

Stem Cell ◽

Quantitative Pcr ◽

Stem Cell Transplant ◽

Diagnostic Value ◽

Haematopoietic Stem Cell Transplant ◽

Haematopoietic Stem Cell ◽

Cell Transplant ◽

Transplant Recipients ◽

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Development of quantitative PCR methods to analyse neural progenitor cell culture state

Biotechnology and Applied Biochemistry ◽

10.1042/ba20050218 ◽

2006 ◽

Vol 44 (1) ◽

pp. 1 ◽

Author(s):

Analeah O'Neill ◽

Elsa Abranches ◽

Matthew J. Robertson ◽

David V. Schaffer ◽

Joaquim M. S. Cabral

Keyword(s):

Cell Culture ◽

Quantitative Pcr ◽

Progenitor Cell ◽

Neural Progenitor Cell ◽

Neural Progenitor

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Evaluation of the inactivation of human Coxsackievirus by thermophilic and mesophilic anaerobic digestion using integrated cell culture and reverse transcription real-time quantitative PCR

Water Research ◽

10.1016/j.watres.2013.04.046 ◽

2013 ◽

Vol 47 (13) ◽

pp. 4259-4264 ◽

Author(s):

Tiejun Gao ◽

Yupin Tong ◽

Ming Cao ◽

Xiaomei Li ◽

Xiaoli Pang

Keyword(s):

Cell Culture ◽

Anaerobic Digestion ◽

Real Time ◽

Quantitative Pcr ◽

Reverse Transcription ◽

Real Time Quantitative Pcr

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Combination of cell culture and quantitative PCR for screening of drugs against Cryptosporidium parvum

Veterinary Parasitology ◽

10.1016/j.vetpar.2009.03.009 ◽

2009 ◽

Vol 162 (3-4) ◽

pp. 271-277 ◽

Author(s):

M. Shahiduzzaman ◽

V. Dyachenko ◽

A. Obwaller ◽

S. Unglaube ◽

A. Daugschies

Keyword(s):

Cell Culture ◽

Quantitative Pcr ◽

Cryptosporidium Parvum

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Development of a combined in vitro cell culture – Quantitative PCR assay for evaluating the disinfection performance of pulsed light for treating the waterborne enteroparasite Giardia lamblia

Experimental Parasitology ◽

10.1016/j.exppara.2014.06.001 ◽

2014 ◽

Vol 144 ◽

pp. 6-13 ◽

Author(s):

Mary Garvey ◽

Alessia Stocca ◽

Neil Rowan

Keyword(s):

Cell Culture ◽

Quantitative Pcr ◽

Giardia Lamblia ◽

Pulsed Light ◽

Pcr Assay ◽

In Vitro Cell Culture ◽

Quantitative Pcr Assay

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