First Report on Efficacy of Citrus limetta Seed Oil in Controlling Cattle Tick Rhipicephalus microplus in Red Sahiwal Calves

2021 ◽  
pp. 109508
Author(s):  
Parag Jain ◽  
Trilochan Satapathy ◽  
Ravindra Kumar Pandey
Keyword(s):  
Seed Oil ◽  
Rhipicephalus Microplus ◽  
Cattle Tick ◽  
First Report ◽  
Citrus Limetta

Acaricidal activity and biochemical analysis of Citrus limetta seed oil for controlling Ixodid Tick Rhipicephalus microplus infesting cattle

2021 ◽  
Author(s):  
Parag Jain ◽  
Trilochan Satapathy ◽  
Ravindra Kumar Pandey
Keyword(s):  
Biochemical Analysis ◽  
Seed Oil ◽  
Ixodid Tick ◽  
Rhipicephalus Microplus ◽  
Acaricidal Activity ◽  
Cattle Tick ◽  
Control Group ◽  
Soxhlet Apparatus ◽  
Citrus Limetta

The present study was performed with an objective to determine acaricidal activity of Citrus limetta seed oil (CLO) against cattle tick Rhipicephalus microplus. The mode of action of CLO was determined by evaluating biochemical enzymatic levels in treated ticks. CLO was obtained by extracting with n-hexane as solvent in soxhlet apparatus. The active compounds in CLO were analyzed through Gas chromatography. Acaricidal activity of CLO was evaluated using larval packet test (LPT) and adult immersion test (AIT). Results of chromatographic analysis showed that palmitic acid, stearic acid, α-Tocopherol and bergamol were present as major active constituents along with other compounds. The lethal concentration (LC50) of CLO was found to be 2.87% and 3.96% for larvae and adult of R. microplus ticks, respectively. In vitro acaricidal bioassay revealed 100% mortality of ticks in both LPT and AIT at 12.5% dose. CLO significantly (p < 0.001) reduced oviposition rate, hatching ability of eggs and reproduction efficiency of ticks. Biochemical analysis of ticks homogenate showed different enzymatic levels. The results revealed that CLO disturbed protective enzymes functions by reducing level of SOD, GST, MAO and AChE but increasing NOS level in ticks as compared with control group. Thus, CLO may be used as an effective acaricidal agent in controlling R. microplus ticks.  

First report of the cattle tick Rhipicephalus microplus resistant to ivermectin in Mexico

2010 ◽  
Vol 168 (1-2) ◽  
pp. 165-169 ◽  
Author(s):  
L.C. Perez-Cogollo ◽  
R.I. Rodriguez-Vivas ◽  
G.T. Ramirez-Cruz ◽  
R.J. Miller
Keyword(s):  
Rhipicephalus Microplus ◽  
Cattle Tick ◽  
First Report

scholarly journals Efficacy of two commercial synthetic pyrethroids (cypermethrin and deltamethrin) on Amblyomma variegatum and Rhipicephalus microplus strains of the south-western region of Burkina Faso

2021 ◽  
Vol 53 (3) ◽  
Author(s):  
Achille S. Ouedraogo ◽  
Olivier M. Zannou ◽  
Abel S. Biguezoton ◽  
Kouassi Yao Patrick ◽  
Adrien M. G. Belem ◽  
...  
Keyword(s):  
Burkina Faso ◽  
Pyrethroid Resistance ◽  
Tick Control ◽  
Rhipicephalus Microplus ◽  
Amblyomma Variegatum ◽  
Synthetic Pyrethroid ◽  
Cattle Tick ◽  
Control Problems ◽  
Synthetic Pyrethroids ◽  
Western Region

AbstractSince 2011, period of the livestock invasion by the cattle tick Rhipicephalus microplus in Burkina Faso (BF), tick-control problems were exacerbated. Based on farmer’s reports, most commonly used commercial acaricides were found to be ineffective in Western South part of the country. To investigate the occurrence and extent of such acaricidal ineffectiveness, we performed the standardized larval packet test (LPT) with commercial deltamethrin (vectocid) and cypermethrin (cypertop), on two cattle tick species, the native Amblyomma variegatum and the invasive R. microplus. The resistance ratios (RR) were computed with susceptible Hounde strain of Rhipicephalus geigyi as reference. The R. microplus population showed resistance to the two acaricides tested with the highest lethal concentration (LC) values, and different resistance ratios higher than 4 (deltamethrin: RR50 = 28.18 and RR90 = 32.41; cypermethrin: RR50 = 8.79 and RR90 = 23.15). In the contrary, A. variegatum population was found to be highly susceptible to acaricides tested with low lethal concentrations and resistance ratio values (deltamethrin: RR50 = 0.5 and RR90 = 0.48; cypermethrin: RR50 = 0.68 and RR90 = 0.79). These data demonstrate high synthetic pyrethroid resistance in R. microplus strain, leading to conclude that the acaricide ineffectiveness in tick populations control remains a concern in BF.

scholarly journals Acaricidal activity of leaves of Morus nigra against the cattle tick Rhipicephalus microplus

2017 ◽  
Vol 69 (3) ◽  
pp. 523-528 ◽  
Author(s):  
A.C.S. Dantas ◽  
D.P. Freire ◽  
G.R. Souza ◽  
J.R.G.S. Almeida ◽  
L.A. Rolim ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Ethanolic Extract ◽  
Adult Tick ◽  
Immersion Test ◽  
Rhipicephalus Microplus ◽  
Acaricidal Activity ◽  
Cattle Tick ◽  
Chloroform Fraction ◽  
Morus Nigra ◽  
Cattle Ticks

ABSTRACT The acaricidal activity of crude ethanolic extract and fractions from the leaves of Morus nigra (Moraceae) was carried out on female cattle ticks Rhipicephalus microplus, using the adult immersion test. The mortality and fertility of females exposed to different concentrations of hexane, chloroform and ethyl acetate fractions, as well as ethanolic extract of M. nigra with concentrations of 5, 10 and 25mg/ml were evaluated using three treatment groups, two control groups and triplicate tests. The study also identified the main phenolic compounds of the extract and fractions of this species by HPLC. The chloroform fraction of leaves of M. nigra (25mg/mL) showed the best results for this species, obtaining 62.6% of inhibition of oviposition, 39.3% of eggs eclosion average and 65.4% of effectiveness. HPLC analysis revealed the presence of phenolic compounds, which may be related to biological activity shown by the extracts, which can be used as an alternative control against R. microplus adult tick.

scholarly journals Mutation in the Rm AOR gene is associated with amitraz resistance in the cattle tick Rhipicephalus microplus

2013 ◽  
Vol 110 (42) ◽  
pp. 16772-16777 ◽  
Author(s):  
S. W. Corley ◽  
N. N. Jonsson ◽  
E. K. Piper ◽  
C. Cutulle ◽  
M. J. Stear ◽  
...  
Keyword(s):  
Rhipicephalus Microplus ◽  
Cattle Tick

scholarly journals Draft Genome Sequence of Enterococcus casseliflavus PAVET15 Obtained from the Oviduct Infection of the Cattle Tick (Rhipicephalus microplus) in Jiutepec, Morelos, Mexico

2017 ◽  
Vol 5 (16) ◽  
Author(s):  
R. Cossío-Bayúgar ◽  
E. Miranda-Miranda ◽  
C. A. Arreguín-Pérez ◽  
L. Lozano ◽  
D. Peréz de la Rosa ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Genome Sequence ◽  
Draft Genome ◽  
Rhipicephalus Microplus ◽  
Draft Genome Sequence ◽  
Cattle Tick ◽  
Gram Positive ◽  
Content Type ◽  
Enterococcus Casseliflavus ◽  
Acid Producing Bacteria

ABSTRACT Enterococcus spp. are Gram-positive lactic acid-producing bacteria found in the intestinal tracts of animals, like mammals, birds, and arthropods. Enterococcus spp. may cause oportunistic infections in vertebrate and invertebrate hosts. We report here the draft genome sequence of Enterococcus casseliflavus PAVET15 containing 3,722,480 bp, with 80 contigs, an N 50 of 179,476 bp, and 41.93% G+C content.

scholarly journals In Vitro Evaluation of Ethanolic Extracts ofAgeratum conyzoidesandArtemisia absinthiumagainst Cattle Tick,Rhipicephalus microplus

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
S. Parveen ◽  
R. Godara ◽  
R. Katoch ◽  
A. Yadav ◽  
P. K. Verma ◽  
...  
Keyword(s):  
Immersion Test ◽  
Rhipicephalus Microplus ◽  
Acaricidal Activity ◽  
Cattle Tick ◽  
Egg Mass ◽  
Ageratum Conyzoides ◽  
Artemisia Absinthium ◽  
Aerial Parts ◽  
Ethanolic Extracts

In vitro efficacy of ethanolic extracts obtained from the aerial parts ofAgeratum conyzoidesandArtemisia absinthiumwas assessed onRhipicephalus microplususing adult immersion test (AIT). Five concentrations of the extract (1.25%, 2.5%, 5%, 10%, and 20%) with three replications for each concentration were used in the bioassay. In AIT, the maximum mortality was recorded as 40% and 66.7% at 20% concentration forA. conyzoidesandA. absinthium, respectively. Acaricidal activity was found to be higher in the extract ofA. absinthiumwith LC50and LC95values of 11.2% and 61.7%, respectively. Egg mass weight of the live ticks treated with different concentrations of the extracts was significantly(P<0.05)lower than that of control ticks; consequently, the reproductive index and oviposition values of the treated ticks were reduced significantly(P<0.05). TheA. conyzoidesinhibited 90% hatching of eggs at the 20% concentration, whereasA. absinthiumshowed 100% inhibition at 5%, 10%, and 20% concentrations. The results show thatA. absinthiumhas better acaricidal properties thanA. conyzoidesand could be useful in controllingR. microplus.

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