scholarly journals Open reading frame 193R of Chilo iridescent virus encodes a functional inhibitor of apoptosis (IAP)

Virology ◽  
2008 ◽  
Vol 376 (1) ◽  
pp. 124-131 ◽  
Author(s):  
İkbal Agah İnce ◽  
Marcel Westenberg ◽  
Just M. Vlak ◽  
Zihni Demirbağ ◽  
Remziye Nalçacıoğlu ◽  
...  
Keyword(s):  
Open Reading Frame ◽  
Inhibitor Of Apoptosis ◽  
Chilo Iridescent Virus ◽  
Reading Frame ◽  
Iridescent Virus

The Apoptosis Inhibitor Gene API2 and a Novel 18q Gene,MLT, Are Recurrently Rearranged in the t(11;18)(q21;q21) Associated With Mucosa-Associated Lymphoid Tissue Lymphomas

Blood ◽  
1999 ◽  
Vol 93 (11) ◽  
pp. 3601-3609 ◽  
Author(s):  
Judith Dierlamm ◽  
Mathijs Baens ◽  
Iwona Wlodarska ◽  
Margarita Stefanova-Ouzounova ◽  
Jesus Maria Hernandez ◽  
...  
Keyword(s):  
Lymphoid Tissue ◽  
Marginal Zone ◽  
Open Reading Frame ◽  
Inhibitor Of Apoptosis ◽  
Low Grade ◽  
Gene Encoding ◽  
Reading Frame ◽  
Genetic Lesion ◽  
Zone Cell ◽  
Apoptosis Inhibitor

Marginal zone cell lymphomas of the mucosa-associated lymphoid tissue (MALT) are the most common subtype of lymphoma arising at extranodal sites. The t(11;18)(q21;q21) appears to be the key genetic lesion and is found in approximately 50% of cytogenetically abnormal low-grade MALT lymphomas. We show that the API2 gene, encoding an inhibitor of apoptosis also known as c-IAP2, HIAP1, andMIHC, and a novel gene on 18q21 characterized by several Ig-like C2-type domains, named MLT, are recurrently rearranged in the t(11;18). In both MALT lymphomas analyzed, the breakpoint inAPI2 occurred in the intron separating the exons coding respectively for the baculovirus IAP repeat domains and the caspase recruitment domain. The breakpoints within MLT differed but the open reading frame was conserved in both cases. In one case, the translocation was accompanied by a cryptic deletion involving the 3′ part of API2. As a result, the reciprocal transcript was not present, strongly suggesting that the API2-MLT fusion is involved in the oncogenesis of MALT lymphoma.

The Apoptosis Inhibitor Gene API2 and a Novel 18q Gene,MLT, Are Recurrently Rearranged in the t(11;18)(q21;q21) Associated With Mucosa-Associated Lymphoid Tissue Lymphomas

Blood ◽  
1999 ◽  
Vol 93 (11) ◽  
pp. 3601-3609 ◽  
Author(s):  
Judith Dierlamm ◽  
Mathijs Baens ◽  
Iwona Wlodarska ◽  
Margarita Stefanova-Ouzounova ◽  
Jesus Maria Hernandez ◽  
...  
Keyword(s):  
Lymphoid Tissue ◽  
Marginal Zone ◽  
Open Reading Frame ◽  
Inhibitor Of Apoptosis ◽  
Low Grade ◽  
Gene Encoding ◽  
Reading Frame ◽  
Genetic Lesion ◽  
Zone Cell ◽  
Apoptosis Inhibitor

Abstract Marginal zone cell lymphomas of the mucosa-associated lymphoid tissue (MALT) are the most common subtype of lymphoma arising at extranodal sites. The t(11;18)(q21;q21) appears to be the key genetic lesion and is found in approximately 50% of cytogenetically abnormal low-grade MALT lymphomas. We show that the API2 gene, encoding an inhibitor of apoptosis also known as c-IAP2, HIAP1, andMIHC, and a novel gene on 18q21 characterized by several Ig-like C2-type domains, named MLT, are recurrently rearranged in the t(11;18). In both MALT lymphomas analyzed, the breakpoint inAPI2 occurred in the intron separating the exons coding respectively for the baculovirus IAP repeat domains and the caspase recruitment domain. The breakpoints within MLT differed but the open reading frame was conserved in both cases. In one case, the translocation was accompanied by a cryptic deletion involving the 3′ part of API2. As a result, the reciprocal transcript was not present, strongly suggesting that the API2-MLT fusion is involved in the oncogenesis of MALT lymphoma.

scholarly journals The cDNA for rat selenoprotein P contains 10 TGA codons in the open reading frame

1991 ◽  
Vol 266 (16) ◽  
pp. 10050-10053
Author(s):  
K.E. Hill ◽  
R.S. Lloyd ◽  
J.G. Yang ◽  
R. Read ◽  
R.F. Burk
Keyword(s):  
Open Reading Frame ◽  
Selenoprotein P ◽  
Reading Frame

scholarly journals The Cloning and Molecular Analysis of pawn-B in Paramecium tetraurelia

Genetics ◽  
2000 ◽  
Vol 155 (3) ◽  
pp. 1105-1117 ◽  
Author(s):  
W John Haynes ◽  
Kit-Yin Ling ◽  
Robin R Preston ◽  
Yoshiro Saimi ◽  
Ching Kung
Keyword(s):  
Genomic Library ◽  
Open Reading Frame ◽  
Paramecium Tetraurelia ◽  
Wild Type ◽  
Rt Pcr ◽  
Reading Frame ◽  
Close Proximity ◽  
In The Wild ◽  
Dna Fragment ◽  
Alternative Sites

Abstract Pawn mutants of Paramecium tetraurelia lack a depolarization-activated Ca2+ current and do not swim backward. Using the method of microinjection and sorting a genomic library, we have cloned a DNA fragment that complements pawn-B (pwB/pwB). The minimal complementing fragment is a 798-bp open reading frame (ORF) that restores the Ca2+ current and the backward swimming when expressed. This ORF contains a 29-bp intron and is transcribed and translated. The translated product has two putative transmembrane domains but no clear matches in current databases. Mutations in the available pwB alleles were found within this ORF. The d4-95 and d4-96 alleles are single base substitutions, while d4-662 (previously pawn-D) harbors a 44-bp insertion that matches an internal eliminated sequence (IES) found in the wild-type germline DNA except for a single C-to-T transition. Northern hybridizations and RT-PCR indicate that d4-662 transcripts are rapidly degraded or not produced. A second 155-bp IES in the wild-type germline ORF excises at two alternative sites spanning three asparagine codons. The pwB ORF appears to be separated from a 5′ neighboring ORF by only 36 bp. The close proximity of the two ORFs and the location of the pwB protein as indicated by GFP-fusion constructs are discussed.

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