Identification of an amino acid domain encoded by the capsid gene of porcine circovirus type 2 that modulates intracellular viral protein distribution during replication

2011 ◽  
Vol 155 (1) ◽  
pp. 358-362 ◽  
Author(s):  
Andrew K. Cheung ◽  
Justin J. Greenlee
Keyword(s):  
Amino Acid ◽  
Viral Protein ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Capsid Gene ◽  
Porcine Circovirus ◽  
Protein Distribution ◽  
Acid Domain ◽  
Amino Acid Domain

scholarly journals Immunogenicity and Pathogenicity of Chimeric Infectious DNA Clones of Pathogenic Porcine Circovirus Type 2 (PCV2) and Nonpathogenic PCV1 in Weanling Pigs

2003 ◽  
Vol 77 (20) ◽  
pp. 11232-11243 ◽  
Author(s):  
M. Fenaux ◽  
T. Opriessnig ◽  
P. G. Halbur ◽  
X. J. Meng
Keyword(s):  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Capsid Gene ◽  
Porcine Circovirus ◽  
Control Group ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Clone Group

ABSTRACT Porcine circovirus type 2 (PCV2) is the primary causative agent of postweaning multisystemic wasting syndrome (PMWS), whereas the ubiquitous porcine circovirus type 1 (PCV1) is nonpathogenic for pigs. We report here the construction and characterization of two chimeric infectious DNA clones of PCV1 and PCV2. The chimeric PCV1-2 clone contains the PCV2 capsid gene cloned in the backbone of the nonpathogenic PCV1 genome. A reciprocal chimeric PCV2-1 DNA clone was also constructed by replacing the PCV2 capsid gene with that of PCV1 in the backbone of the PCV2 genome. The PCV1, PCV2, and chimeric PCV1-2 and PCV2-1 DNA clones were all shown to be infectious in PK-15 cells, and their growth characteristics in vitro were determined and compared. To evaluate the immunogenicity and pathogenicity of the chimeric infectious DNA clones, 40 specific-pathogen-free (SPF) pigs were randomly assigned into five groups of eight pigs each. Group 1 pigs received phosphate-buffered saline as the negative control. Group 2 pigs were each injected in the superficial inguinal lymph nodes with 200 μg of the PCV1 infectious DNA clone. Group 3 pigs were each similarly injected with 200 μg of the PCV2 infectious DNA clone, group 4 pigs were each injected with 200 μg of the chimeric PCV1-2 infectious DNA clone, and group 5 pigs were each injected with 200 μg of the reciprocal chimeric PCV2-1 infectious DNA clone. As expected, seroconversion to antibodies to the PCV2 capsid antigen was detected in group 3 and group 4 pigs. Group 2 and 5 pigs all seroconverted to PCV1 antibody. Gross and microscopic lesions in various tissues of animals inoculated with the PCV2 infectious DNA clone were significantly more severe than those found in pigs inoculated with PCV1, chimeric PCV1-2, and reciprocal chimeric PCV2-1 infectious DNA clones. These data indicated that the chimeric PCV1-2 virus with the immunogenic ORF2 capsid gene of pathogenic PCV2 cloned into the nonpathogenic PCV1 genomic backbone induces a specific antibody response to the pathogenic PCV2 capsid antigen but is attenuated in pigs. Future studies are warranted to evaluate the usefulness of the chimeric PCV1-2 infectious DNA clone as a genetically engineered live-attenuated vaccine against PCV2 infection and PMWS.

Porcine circovirus type 2 affects the serum profile of amino acids and intestinal expression of amino acid transporters in mice

RSC Advances ◽  
2015 ◽  
Vol 5 (90) ◽  
pp. 73651-73659 ◽  
Author(s):  
Dingfu Xiao ◽  
Jie Yin ◽  
Wenkai Ren ◽  
Jianhua He ◽  
Xionggui Hu ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Amino Acid Transporters ◽  
Highly Pathogenic ◽  
Serum Amino Acids ◽  
Amino Acids Profile

PCV2 is highly pathogenic, however, its effect on the serum amino acids profile is unknown.

Amino acid mutations in the capsid protein produce novel porcine circovirus type 2 neutralizing epitopes

2013 ◽  
Vol 165 (3-4) ◽  
pp. 260-267 ◽  
Author(s):  
Jianbo Liu ◽  
Liping Huang ◽  
Yanwu Wei ◽  
Qinghai Tang ◽  
Dan Liu ◽  
...  
Keyword(s):  
Amino Acid ◽  
Capsid Protein ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Amino Acid Mutations ◽  
Neutralizing Epitopes

Study of the genetic variability of porcine circovirus type 2 detected in Serbia and Slovenia

2012 ◽  
Vol 60 (3) ◽  
pp. 409-420 ◽  
Author(s):  
Ivan Toplak ◽  
Sava Lazić ◽  
Diana Lupulović ◽  
Jasna Prodanov-Radulović ◽  
Zsolt Becskei ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Position ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Amino Acid Sequences ◽  
Porcine Circovirus ◽  
Variable Regions ◽  
Predominant Genotype ◽  
Major Variable

Recent variants of porcine circovirus type 2 (PCV2) were obtained from tissues of domestic pigs with porcine circovirus associated disease and from randomly selected wild boar samples from Serbia and Slovenia. A 450-base-pair nucleotide sequence was obtained by PCR from the ORF2. The derived nucleotide and amino acid sequences were aligned and compared to the corresponding region of closely related PCV2 sequences determined in previous years and retrieved from the GenBank. The 30 Serbian and 17 Slovenian PCV2 sequences clustered into three previously determined genotypes (PCV2a: 7), (PCV2b: 38) and (PCV2d: 2). Three major variable regions, concerning 29 amino acid position substitutions within the ORF2, were observed, which further supports the segregation of the detected strains into three separate genotypes. This study indicates that PCV2b is the predominant genotype in Serbia and Slovenia and the detected PCV2 strains are closely related to those previously described in Europe and in other parts of the world.

scholarly journals Phylogenetic analysis of porcine circovirus type 2 (PCV2) between 2015 and 2018 in Henan Province, China

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Guanmin Zheng ◽  
Qingxia Lu ◽  
Fangyu Wang ◽  
Guangxu Xing ◽  
Hua Feng ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Amino Acid ◽  
Complete Genome ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Henan Province ◽  
Porcine Circovirus ◽  
Economic Losses ◽  
Clinical Samples

Abstract Background Porcine circovirus type 2 (PCV2) is the pathogen of porcine circovirus associated diseases (PCVAD) and one of the main pathogens in the global pig industry, which has brought huge economic losses to the pig industry. In recent years, there has been limited research on the prevalence of PCV2 in Henan Province. This study investigated the genotype and evolution of PCV2 in this area. Results We collected 117 clinical samples from different regions of Henan Province from 2015 to 2018. Here, we found that the PCV2 infection rate of PCV2 was 62.4%. Thirty-seven positive clinical samples were selected to amplify the complete genome of PCV2 and were sequenced. Based on the phylogenetic analysis of PCV2 ORF2 and complete genome, it was found that the 37 newly detected strains belonged to PCV2a (3 of 37), PCV2b (21 of 37) and PCV2d (13 of 37), indicating the predominant prevalence of PCV2b and PCV2d strains. In addition, we compared the amino acid sequences and found several amino acid mutation sites among different genotypes. Furthermore, the results of selective pressure analysis showed that there were 5 positive selection sites. Conclusions This study indicated the genetic diversity, molecular epidemiology and evolution of PCV2 genotypes in Henan Province during 2015–2018.

Evaluation of the enhancing ability of three adjuvants for DNA vaccination using the porcine circovirus type 2 ORF2 (capsid) gene in mice

2013 ◽  
Vol 171 (1) ◽  
pp. 247-251 ◽  
Author(s):  
Fang Fu ◽  
Yuekun Lang ◽  
Xuesong Li ◽  
Zheng Chai ◽  
Guangzhi Tong ◽  
...  
Keyword(s):  
Dna Vaccination ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Capsid Gene ◽  
Porcine Circovirus

scholarly journals Mutant USA strain of porcine circovirus type 2 (mPCV2) exhibits similar virulence to the classical PCV2a and PCV2b strains in caesarean-derived, colostrum-deprived pigs

2014 ◽  
Vol 95 (11) ◽  
pp. 2495-2503 ◽  
Author(s):  
Tanja Opriessnig ◽  
Chao-Ting Xiao ◽  
Priscilla F. Gerber ◽  
Patrick G. Halbur ◽  
Shannon R. Matzinger ◽  
...  
Keyword(s):  
Amino Acid ◽  
Infectious Virus ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Significant Difference ◽  
The Usa ◽  
Gross Lesions ◽  
Additional Amino Acid

In 2012, a mutant porcine circovirus type 2 (mPCV2) strain was identified in cases of PCV-associated disease (PCVAD) in the USA. The mPCV2 had an additional amino acid, lysine (K), in the capsid at position 234. The objectives of this study were to compare the pathogenicity of mPCV2, PCV2a and PCV2b in pigs using biologically pure infectious virus stocks derived from respective infectious DNA clones, and to investigate the importance of genotype-specific ORF2 and the presence of lysine at position 234 of the capsid. A total of 47, 2-week-old, caesarean-derived, colostrum-deprived (CDCD) pigs were assigned to one of seven groups. At 3 weeks of age, the pigs were experimentally inoculated with saline, PCV2a, PCV2b, mPCV2, PCV2b-234-K (lysine addition in ORF2), chimeric PCV2b-ORF1/mPCV2-ORF2 or reciprocal chimeric mPCV2-ORF1/PCV2b-ORF2. All pigs were necropsied 21 days post-infection (p.i.). Gross lesions were limited to visible icterus and loss of body condition in a portion of the mPCV2 pigs. The amount of PCV2 DNA was significantly higher in pigs inoculated with mPCV2 compared with PCV2b in sera at 7 days p.i. and faecal swabs at 14 days p.i. Based on lymphoid lesions, a higher prevalence of PCVAD was seen in pigs infected with PCV2s containing the additional 234-K (64.3 %) compared with those infected with a PCV2 with the regular 233 bp ORF2 (40 %). Results indicated that all PCV2 isolates were capable of inducing severe lesions and disease in the CDCD pig model, and there was no significant difference in virulence.

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