Phylogenetic analysis of VP1, VP2, and VP3 gene segments of genotype G5 group A rotavirus strains circulating in Brazil between 1986 and 2005

2011 ◽  
Vol 160 (1-2) ◽  
pp. 381-388 ◽  
Author(s):  
Marcelle Figueira Marques da Silva ◽  
Luis Fernando López Tort ◽  
Mariela Martínez Gómez ◽  
Rosane M. Santos Assis ◽  
Marcos César Lima de Mendonça ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Group A Rotavirus ◽  
Group A ◽  
Vp3 Gene

scholarly journals Isolation and characterization of a rare group A rotavirus G13P[18] strain from a diarrhoeic foal in Japan

2020 ◽  
Vol 101 (8) ◽  
pp. 800-805
Author(s):  
Manabu Nemoto ◽  
Hidekazu Niwa ◽  
Hiroshi Kida ◽  
Tohru Higuchi ◽  
Yasuhiro Orita ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Group A Rotavirus ◽  
Isolation And Characterization ◽  
Genotype Constellation ◽  
Group A ◽  
First Time ◽  
Rare Group

A rare genotype G13P[18] group A rotavirus (RVA/Horse-tc/JPN/MK9/2019/G13P[18]) was isolated from a diarrhoeic foal for the first time in 28 years. The genotype constellation of the virus was assigned to G13-P[18]-I6-R9-C9-M6-A6-N9-T12-E14-H11 and was the same as that of the first isolated strain, RVA/Horse-tc/GBR/L338/1991/G13P[18]. Phylogenetic analysis suggests that the virus is related to RVA/Horse-tc/GBR/L338/1991/G13P[18] and is distant from typical equine rotaviruses of the G3P[12] and G14P[12] genotypes.

Phylogenetic analysis of G1P[6] group A rotavirus strains detected in Northeast Brazilian children fully vaccinated with Rotarix™

2013 ◽  
Vol 19 ◽  
pp. 395-402 ◽  
Author(s):  
Mariela Martínez Gómez ◽  
Marcelle Figueira Marques da Silva ◽  
Mark Zeller ◽  
Elisabeth Heylen ◽  
Jelle Matthijnssens ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Group A Rotavirus ◽  
Group A ◽  
Brazilian Children

scholarly journals Phylogenetic analysis of partial VP7 gene of the emerging human group A rotavirus G12 strains circulating in Tunisia

2017 ◽  
Vol 66 (2) ◽  
pp. 112-118 ◽  
Author(s):  
Amal Moussa ◽  
Mouna Ben Hadj Fredj ◽  
Meriam BenHamida-Rebaï ◽  
Imene Fodha ◽  
Noureddine Boujaafar ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Human Group ◽  
Group A Rotavirus ◽  
Group A ◽  
Vp7 Gene

Molecular Detection of Group A Rotavirus in under Five Years Old Children with Acute Diarrhoea Admitted to Yangon Children’s Hospital

2019 ◽  
Vol 31 (1) ◽  
pp. 87-92
Keyword(s):  
Acute Diarrhoea ◽  
Enzyme Linked Immunosorbent Assay ◽  
Viral Gastroenteritis ◽  
Cross Sectional ◽  
Under Five ◽  
Group A Rotavirus ◽  
Rotavirus Vaccination ◽  
Group A ◽  
Stool Samples ◽  
Considerable Morbidity

Rotaviruses are regarded as the most common cause of viral gastroenteritis and are responsible for considerable morbidity and mortality among children especially under five years of age worldwide. In developing countries like Myanmar, where diarrhoea is in the priority childhood disease, rotavirus surveillance and detection of rotavirus genotypes are utmost important. A hospital-based, cross-sectional descriptive study was conducted at Yangon Children‟s Hospital among under five children admitted for acute diarrhoea from January to October 2016. This study includes detection of Group A rotavirus antigen by commercial enzyme-linked immunosorbent assay (ELISA) and genotyping by multiplex RT-PCR. From a total of 488 collected samples, rotavirus antigen was detected in 219 samples (45%). Rotavirus diarrhoea was most common among the age of 6-11 months (38.8%) followed by 12-23 months (37.9%). The results showed that boys were more commonly affected than girls. Detection of rotavirus positivity was peak in February (57.6 %). Out of 219 stool samples with positive ELISA result, 40 stool samples with high optical density value were proceeded for further determination of G and P genotypes. Regarding distribution of G genotypes, the most common G genotype was G9 which comprised 45%, and that of P genotype was P[8] which comprised 92.5%. Regarding combination of G and P genotypes, the most frequent combination is G9P[8], and it constituted 42.5%. Untypable genotypes were seen in 30% of G and 2.5% of P typing. As rotavirus infection can be prevented by vaccine, WHO recommended that rotavirus vaccination should be included in national immunization program especially in countries where prevalence of rotavirus is high. The distribution of G and P genotypes is important in consideration of appropriate vaccine in pre-vaccination and evaluation of effectiveness of vaccine in post-vaccination period. Therefore, the information on currently circulating genotypes of rotavirus in this study will serve as valuable data for vaccination programme.

ROTADIAL: the first Nanobody-based immunoassay to detect Group A Rotavirus

2021 ◽  
pp. 114279
Author(s):  
C.G. Vega ◽  
L.L. Garaicoechea ◽  
J.I. Degiuseppe ◽  
M. Bok ◽  
A.A. Rivolta ◽  
...  
Keyword(s):  
Group A Rotavirus ◽  
Group A

scholarly journals Prevalence and Genetic Diversity of Group A Rotavirus Genotypes in Moscow (2019–2020)

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 674
Author(s):  
Anton Yuzhakov ◽  
Ksenia Yuzhakova ◽  
Nadezhda Kulikova ◽  
Lidia Kisteneva ◽  
Stanislav Cherepushkin ◽  
...  
Keyword(s):  
Infectious Disease ◽  
Genetic Diversity ◽  
Acute Gastroenteritis ◽  
Rt Pcr ◽  
Clinical Hospital ◽  
Group A Rotavirus ◽  
Group A ◽  
First Time ◽  
Common Combination ◽  
Children Under 5

Group A rotavirus (RVA) infection is the leading cause of hospitalization of children under 5 years old, presenting with symptoms of acute gastroenteritis. The aim of our study was to explore the genetic diversity of RVA among patients admitted to Moscow Infectious Disease Clinical Hospital No. 1 with symptoms of acute gastroenteritis. A total of 653 samples were collected from May 2019 through March 2020. Out of them, 135 (20.67%) fecal samples were found to be positive for rotavirus antigen by ELISA. RT-PCR detected rotavirus RNA in 80 samples. Seven G-genotypes (G1, G2, G3, G4, G8, G9, and G12) and three P-genotypes (P[8], P[4], and P[6]) formed 9 different combinations. The most common combination was G9P[8]. However, for the first time in Moscow, the combination G3P[8] took second place. Moreover, all detected viruses of this combination belonged to Equine-like G3P[8] viruses that had never been detected in Russia before. The genotype G8P[8] and G9P[4] rotaviruses were also detected in Moscow for the first time. Among the studied rotaviruses, there were equal proportions of Wa and DS-1-like strains; previous studies showed that Wa-like strains accounted for the largest proportion of rotaviruses in Russia.

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