A temperature-driven model for potato yellow vein virus transmission efficacy by Trialeurodes vaporariorum (Hemiptera: Aleyrodidae)

Potato yellow vein virus (PYVV) is the causal agent of the potato yellow vein disease and can reduce potato production up to 50%. This virus also infects tomatoes and can remain asymptomatic in plants. PYVV transmission is mediated by vegetative seed, the vector Trialeurodes vaporariorum, and grafts. Its genome has the P26 and P10 genes that are orthologues in the Crinivirus genus, which have been characterized as pathogenic factors and have not been studied in PYVV. We analyzed the variability of P26 and P10 from 45 and 48 sequences, which were obtained by RT-PCR amplification of the total RNA of symptomatic potato leaves from the provinces of Nariño, Cundinamarca, and Boyaca (Colombia). We included sequences of each gene of the PYVV genome of potato and tomato isolates from GenBank. The variability in these genes is influenced by the flow and uncontrolled use of vegetative seed between different provinces, that favor the dispersion of viral variants. In addition, the variability analysis based on maximum likelihood trees, haplotypes, and diversity indices showed that P26 is more variable than P10 and both are more variable in Andigena than in Phureja potatoes. The Tajima and Fu and Li tests revealedthat these genes are subject to negative selection.

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Analysis of the RNA of Potato yellow vein virus: evidence for a tripartite genome and conserved 3′-terminal structures among members of the genus Crinivirus

Journal of General Virology ◽

10.1099/vir.0.79910-0 ◽

2004 ◽

Vol 85 (7) ◽

pp. 2065-2075 ◽

Cited By ~ 45

Author(s):

I. C. Livieratos ◽

E. Eliasco ◽

G. Müller ◽

R. C. L. Olsthoorn ◽

L. F. Salazar ◽

...

Keyword(s):

Structure Prediction ◽

Secondary Structure Prediction ◽

Gene Array ◽

Distinct Species ◽

Trialeurodes Vaporariorum ◽

Yellow Vein ◽

Northern Hybridization ◽

Stem Loop ◽

Reading Frame ◽

Potato Yellow Vein Virus

Double-stranded RNA preparations produced from potato plants graft-inoculated with a Peruvian isolate of Potato yellow vein virus (PYVV; genus Crinivirus, family Closteroviridae) contain five RNA species denoted RNA 1, RNA 2, RNA 3, x and y of approximately 8, 5·3, 3·8, 2·0 and 1·8 kbp, respectively. The complete nucleotide sequences of PYVV RNAs 1, 2 and 3 and Northern hybridization analysis showed that PYVV RNA 1 contained the replication module and an additional open reading frame (p7), while two distinct species, RNAs 2 and 3, contain the Closteroviridae hallmark gene array. Pairwise comparisons and phylogeny of genome-encoded proteins showed that PYVV shares significant homology with other criniviruses but is most closely related to the Trialeurodes vaporariorum-vectored Cucumber yellows virus. Secondary structure prediction of the 3′-untranslated regions of all three PYVV RNAs revealed four conserved stem–loop structures and a 3′-terminal pseudoknot structure, also predicted for all fully characterized members of the genus Crinivirus and some members of the genera Closterovirus and Ampelovirus.

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Development of real-time and conventional RT-PCR assays for the detection of potato yellow vein virus (PYVV)

Journal of Virological Methods ◽

10.1016/j.jviromet.2006.03.026 ◽

2006 ◽

Vol 136 (1-2) ◽

pp. 24-29 ◽

Cited By ~ 32

Author(s):

R. López ◽

C. Asensio ◽

M.M. Guzman ◽

N. Boonham

Keyword(s):

Real Time ◽

Yellow Vein ◽

Rt Pcr ◽

Pcr Assays ◽

Potato Yellow Vein Virus

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Economic injury levels for the potato yellow vein disease and its vector, Trialeurodes vaporariorum (Hemiptera: Aleyrodidae), affecting potato crops in the Andes

Crop Protection ◽

10.1016/j.cropro.2019.01.002 ◽

2019 ◽

Vol 119 ◽

pp. 52-58 ◽

Cited By ~ 3

Author(s):

Diego F. Rincon ◽

Diego F. Vasquez ◽

Hugo Fernando Rivera-Trujillo ◽

Carlos Beltrán ◽

Felipe Borrero-Echeverry

Keyword(s):

Trialeurodes Vaporariorum ◽

Yellow Vein ◽

The Andes ◽

Economic Injury ◽

Potato Crops ◽

Vein Disease

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First report of recombination in Potato yellow vein virus (PYVV) in Colombia

Tropical Plant Pathology ◽

10.1590/s1982-56762014000300007 ◽

2014 ◽

Vol 39 (3) ◽

pp. 234-241 ◽

Cited By ~ 5

Author(s):

Giovanni Chaves-Bedoya ◽

Karen Cubillos ◽

Mónica Guzmán-Barney

Keyword(s):

Yellow Vein ◽

First Report ◽

Potato Yellow Vein Virus

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Potato Virus Y (PVY) y Potato Yellow Vein Virus (PYVV) en Infecciones Mixtas No Causan Síntomas Atípicos en Plantas de Papa

Revista Facultad de Ciencias Básicas ◽

10.18359/rfcb.1297 ◽

2016 ◽

Vol 11 (2) ◽

pp. 26 ◽

Cited By ~ 2

Author(s):

Jenny Paola Alfaro García ◽

Liliana Franco-Lara

Keyword(s):

Solanum Tuberosum ◽

Potato Virus ◽

Potato Virus Y ◽

Yellow Vein ◽

Solanum Phureja ◽

Rt Pcr ◽

Potato Yellow Vein Virus

Observaciones anteriores en cultivos de papa (Solanum tuberosum y Solanum phureja) mostraron síntomas llamados atípicos en este trabajo, que consistían en hojas con manchas irregulares verde oscuro sobre un fondo amarillo intenso, que sugieren la presencia de virus. Estos síntomas no correlacionan con ningún virus descrito para papa en Colombia. En plantas, existen reportes de infecciones virales con dos o más virus que llevan a interacciones como sinergismo o antagonismo. En este trabajo se evaluó la hipótesis de que los síntomas atípicos podrían ser resultado de infecciones mixtas entre PYVV (Potato yellow vein virus) (Crinivirus) y PVY (Potato virus Y) (Potyirus), pues ambos virus son comunes en cultivos de papa en Colombia. Se reportan resultados de RT-PCR (reacción en cadena de la polimerasa mediada por retrotranscripción) para la detección de PYVV y PVY, y de ELISA para PVX, PVS, PVM y PRLV en 57 plantas con y sin síntomas atípicos provenientes de campo. Los resultados no apoyan la hipótesis planteada, pues de 10 plantas con síntomas atípicos evaluadas solo una estaba infectada con los dos virus. Por otro lado, coinfecciones de PVY y PYVV se observaron en plantas sin síntomas aparentes (4 plantas de 5 evaluadas) y en plantas con síntomas característicos de PYVV (17 plantas de 37 evaluadas). Del total de 20 plantas evaluadas por ELISA, 18 presentaban infección por PVX aunque no se observaron síntomas asociados a este virus. Ocho de estas plantas, además de PVX estaban infectadas también con PVY y PYVV, pero mostraban síntomas de característicos de PYVV, lo que sugiere que PVX tampoco correlaciona con los síntomas atípicos. Se sugiere la presencia de un virus no reportado infectando el cultivo de papa en Colombia.

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Natural co-infection of Solanum tuberosum crops by the Potato yellow vein virus and potyvirus in Colombia

Agronomía Colombiana ◽

10.15446/agron.colomb.v32n2.43968 ◽

2014 ◽

Vol 32 (2) ◽

pp. 213-223 ◽

Cited By ~ 10

Author(s):

Angela Villamil-Garzón ◽

Wilmer J. Cuellar ◽

Mónica Guzmán-Barney

Keyword(s):

Solanum Tuberosum ◽

Potato Virus ◽

Mixed Infection ◽

Disease Diagnosis ◽

Yellow Vein ◽

Breeding Programs ◽

Potato Plants ◽

Next Generation Sequencing Ngs ◽

Vein Disease ◽

Potato Yellow Vein Virus

The Potato yellow vein virus (PYVV), a Crinivirus with an RNA tripartite genome, is the causal agent of the potato yellow vein disease, reported in Colombian since 1950, with yield reductions of up to 50%. Co-infection of two or more viruses is common in nature and can be associated with differences in virus accumulation and symptom expression. No evidence of mixed infection between PYVV and other viruses has been reported. In this study, eight plants showing yellowing PYVV symptoms: four Solanum tuberosum Group Phureja (P) and four Group Andigena (A), were collected in Cundinamarca, Colombia to detect mixed infection in the isolates using next generation sequencing (NGS). The Potato virus Y (PVY) complete genome (similar to N strain) and the Potato virus V (PVV) partial genomes were detected using NGS and re-confirmed by RT-PCR. Preliminary field screening in a large sample showed that PYVV and PVY co-infect potato plants with a prevalence of 21% within the P group and 23% within the A group. This is the first report of co-infection of PYVV and potyvirus in Colombia and with the use of NGS. Considering that potyviruses enhance symptom severity and/or yield reductions in mixed infections, our results may be relevant for disease diagnosis, breeding programs and tuber certification.

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