Molecular characterization of liver fluke intermediate host lymnaeids (Gastropoda: Pulmonata) snails from selected regions of Okavango Delta of Botswana, KwaZulu-Natal and Mpumalanga provinces of South Africa

2019 ◽  
Vol 17 ◽  
pp. 100318
Author(s):  
Mokgadi P. Malatji ◽  
Jennifer Lamb ◽  
Samson Mukaratirwa
2001 ◽  
Vol 20 (2) ◽  
pp. 56-71
Author(s):  
Editorial Office

This paper focuses on the geographical distribution and the habitats of Lymnaea natalensis, the snail intermediate host of the liver fluke, Fasciola gigantica, as reflected by the collection sites of its 4 552 samples currently on record in the National Freshwater Snail Collection (NFSC) of South Africa. Although this species was represented in a variety of waterbodies, the majority of samples(±70%)came from rivers, brooks and dams and in 70.8% of the cases the water was described as permanent and in 71.8% as slow flowing or standing. The results of life-table studies conducted by various authors indicated that temperature should be a relatively unimportant factor in determining its geographical distribution, but that the availability of permanent water should be decisive for its presence in a given habitat. These results are in agreement with the finding that only 7.5% of the samples of this species in the NFSC were collected in habitats which were described as seasonal. Furthermore, it gives a logical explanation for the sporadic occurrence, or total absence of this species in the more arid regions of South Africa. Water impoundments and irrigation networks contribute to a large extent towards creating perennial habitats which would be suitable for L. natalensis. As intermediate host for one of the liver fluke species which already is an economic factor in South Africa, this certainly is an aspect which ought to be reckoned within the planning and construction of new irrigation schemes.


2012 ◽  
Vol 88 (1) ◽  
pp. 123-128 ◽  
Author(s):  
C.C. Appleton

AbstractNine cases of paragonimiasis have been reported from cats (4), dogs (2) and children (3) in South Africa, with an additional suspected case in an adult female patient. Details of these cases are reviewed. All nine cases, and perhaps the adult case as well, were from the province of KwaZulu-Natal but locality data are only available for six of them. These six cases represent four localities which all lie below 100 m above sea level in the province's lowlands, suggesting that there may be a focus of transmission here. The molluscan first intermediate host must be one of the two prosobranch snail species present in the area,Melanoides tuberculataorTomichia natalensis, and the decapod second intermediate host the common river crabPotamonautes sidneyi. All infected cats and dogs had pulmonary infections, while two human cases for which there is sufficient information had extrapulmonary infections. Transmission appears to be ongoing but the invasive snailTarebia graniferamay be competing with bothM. tuberculataandT. natalensis.If so, this may bring transmission to an end.


2009 ◽  
Vol 47 (5) ◽  
pp. 1319-1324 ◽  
Author(s):  
N. Wolter ◽  
M. du Plessis ◽  
A. von Gottberg ◽  
L. de Gouveia ◽  
K. P. Klugman

2021 ◽  
Author(s):  
Renay Ngobeni ◽  
Amidou Samie

Abstract BACKGROUND: Even though E. histolytica is recognized as an effective pathogen, what determines the outcome of this infection is still not well understood. The present study was carried out to determine the genetic characteristics of E. histolytica isolates from two different regions in South Africa. METHOD: Diarrheal and non-diarrheal stool samples were collected from patients of all ages from Giyani and Pretoria. Different PCR protocols were used to identify E. histolytica and amplify the serine rich E. histolytica protein (SREHP) and chitinase genes. The profiles obtained were compared among the different samples.RESULTS: Out of 111 stool samples collected, 51 were positive by either PCR or microscopy and 14 samples were positive by both methods. The serine- rich E. histolytica protein was amplified in 26 samples. Out of the 26 samples (19) different SREHP profiles were obtained. SREHP #2 was obtained in 5 different samples, 4 from Pretoria and 1 from Giyani (2 diarrheal and 3 non-diarrheal). The chitinase gene was amplified from 51 samples and 22 different chitinase profiles were obtained. Of all the profiles, profile #4 was found in 6 different isolates, 5 from Giyani and 1 from Pretoria (3 symptomatic and 3 asymptomatic). However, profile # 18 was only found in formed stools from Giyani. CONCLUSIONS. The results obtained in this study have further confirmed the genetic heterogeneity of E. histolytica for the SREHP and chitinase genes which might have a significant influence in the outcome of amebic infection, depending on the genetic profile of the infecting strain.


2011 ◽  
Vol 62 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Takeshi AGATSUMA ◽  
Sae FUKUNAGA ◽  
Blanca R. JARILLA ◽  
Mitsuru NAGATAKI ◽  
Shinji TOKUHIRO ◽  
...  

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