scholarly journals Tandem mass spectrometry identifies proteins phosphorylated by cyclic AMP-dependent protein kinase when sea urchin sperm undergo the acrosome reaction

2005 ◽  
Vol 285 (1) ◽  
pp. 116-125 ◽  
Author(s):  
Yi-Hsien Su ◽  
Sheng-Hong Chen ◽  
Huilin Zhou ◽  
Victor D. Vacquier
Keyword(s):  
Mass Spectrometry ◽  
Protein Kinase ◽  
Cyclic Amp ◽  
Tandem Mass Spectrometry ◽  
Sea Urchin ◽  
Acrosome Reaction ◽  
Tandem Mass ◽  
Dependent Protein Kinase ◽  
Dependent Protein ◽  
Sea Urchin Sperm

Crosstalk between protein kinases A and C regulates sea urchin sperm motility

Zygote ◽  
2021 ◽  
pp. 1-12
Author(s):  
Arlet Loza-Huerta ◽  
Hiram Pacheco-Castillo ◽  
Alberto Darszon ◽  
Carmen Beltrán
Keyword(s):  
Protein Kinase ◽  
Sperm Motility ◽  
Sea Urchin ◽  
Sea Urchins ◽  
Dependent Protein Kinase ◽  
Kinase C ◽  
Competitive Inhibitors ◽  
Motility Regulation ◽  
Dependent Protein ◽  
Sea Urchin Sperm

Summary Fertilization, a crucial event for species preservation, in sea urchins, as in many other organisms, requires sperm motility regulation. In Strongylocentrotus purpuratus sea urchins, speract, a sperm chemoattractant component released to seawater from the outer egg layer, attracts sperm after binding to its receptor in the sperm flagellum. Previous experiments performed in demembranated sperm indicated that motility regulation in these cells involved protein phosphorylation mainly due to the cAMP-dependent protein kinase (PKA). However, little information is known about the involvement of protein kinase C (PKC) in this process. In this work, using intact S. purpuratus sea urchin sperm, we show that: (i) the levels of both phosphorylated PKA (PKA substrates) and PKC (PKC substrates) substrates change between immotile, motile and speract-stimulated sperm, and (ii) the non-competitive PKA (H89) and PKC (chelerythrine) inhibitors diminish the circular velocity of sperm and alter the phosphorylation levels of PKA substrates and PKC substrates, while the competitive inhibitors Rp-cAMP and bisindolylmaleimide (BIM) do not. Altogether, our results show that both PKA and PKC participate in sperm motility regulation through a crosstalk in the signalling pathway. These results contribute to a better understanding of the mechanisms that govern motility in sea urchin sperm.

Inhibition of mitosis in fertilized sea urchin eggs by inhibition of the cyclic AMP-dependent protein kinase

1990 ◽  
Vol 188 (1) ◽  
pp. 122-128 ◽  
Author(s):  
Carole L. Browne ◽  
William A. Bower ◽  
Robert E. Palazzo ◽  
Lionel I. Rebhun
Keyword(s):  
Protein Kinase ◽  
Cyclic Amp ◽  
Sea Urchin ◽  
Dependent Protein Kinase ◽  
Sea Urchin Eggs ◽  
Dependent Protein
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