Effect of dilution in sperm maturation media and time of storage on sperm motility and fertilizing capacity of cryopreserved semen of sex-reversed female rainbow trout

2017 ◽  
Vol 245 ◽  
pp. 89-93 ◽  
Author(s):  
Sylwia Judycka ◽  
Andrzej Ciereszko ◽  
Stefan Dobosz ◽  
Tomasz Zalewski ◽  
Grzegorz J. Dietrich
2017 ◽  
Vol 29 (7) ◽  
pp. 1319 ◽  
Author(s):  
Olga Bondarenko ◽  
Borys Dzyuba ◽  
Marek Rodina ◽  
Jacky Cosson

The role of Ca2+ in sturgeon sperm maturation and motility was investigated. Sperm from mature male sterlets (Acipenser ruthenus) were collected from the Wolffian duct and testis 24 h after hormone induction. Testicular spermatozoa (TS) were incubated in Wolffian duct seminal fluid (WDSF) for 5 min at 20°C and were designated ‘TS after IVM’ (TSM). Sperm motility was activated in media with different ion compositions, with motility parameters analysed from standard video microscopy records. To investigate the role of calcium transport in the IVM process, IVM was performed (5 min at 20°C) in the presence of 2 mM EGTA, 100 µM Verapamil or 100 µM Tetracaine. No motility was observed in the case of TS (10 mM Tris, 25 mM NaCl, 50 mM Sucr with or without the addition of 2 mM EGTA). Both incubation of TS in WDSF and supplementation of the activation medium with Ca2+ led to sperm motility. The minimal Ca2+ concentration required for motility activation of Wolffian duct spermatozoa, TS and TSM was determined (1–2 nM for Wolffian duct spermatozoa and TSM; approximately 0.6 mM for TS). Motility was obtained after the addition of verapamil to the incubation medium during IVM, whereas the addition of EGTA completely suppressed motility, implying Ca2+ involvement in sturgeon sperm maturation. Further studies into the roles of Ca2+ transport in sturgeon sperm maturation and motility are required.


Author(s):  
Brett Nixon ◽  
Amanda L. Anderson ◽  
Elizabeth G. Bromfield ◽  
Jacinta H. Martin ◽  
Shenae L. Cafe ◽  
...  

2013 ◽  
Vol 13 (2) ◽  
Author(s):  
D. Setiadi Dan S. Bachri

AbstrakIndonesia memiliki banyak tumbuhan berpotensi obat salah satunya bisa dijadikan sebagai obatkontrasepsi tradisonal yang biasa digunakan untuk menjarangkan anak atau sterilisasi sepertirebusan akar Caesalpinia bonduc (Aru). Tujuan penelitian untuk mengetahui pengaruh obatkontrasepsi tradisional air rebusan akar aru terhadap kualitas pematangan sperma epididimis mencit(Mus musculus). Mencit dipilih secara acak untuk mewakili 4 kelompok dosis yaitu : 0,0 (K0), 16,0(K1) 24,0 (K2) dan 32,0 (K3) μl/gram berat badan/hari. Setiap kelompok perlakuan dilakukandengan 5 kali replikasi. Perlakuan diberikan melalui oral dengan menggunakan jarum gavage selama10 hari berturut-turut. Pembedahan dilakukan pada hari pertama setelah perlakuan selesai.Pengamatan kualitas sperma epididimis dengan perhitungan produksi sperma, % sperm motil, %sperma hidup, % sperma abnormal,dan rangking keaktifan sperma. Untuk mengetahui ada atautidaknya perbedaan yang bermakna antar perlakuan dalam kelompok dilakukan uji Anava satu arah,bila terdapat perbedaan bermakna dilanjutkan uji BNJ untuk membandingkan angka rata-rata antarkelompok perlakuan. Pemberian air rebusan akar Caesalpinea bonduc pada mencit menunjukanpengaruh perbedaan secara signifikan pada jumlah (%) sperma abnormal antara dosis 0,0 μl dengan24,0 dan 32,0 μl serta antara 16,0 μl dengan 24,0 dan 32,0 μl; Jumlah (%) sperma hidup terdapatperbedaan antara dosis 0,0 μl dengan 16,0 , 24,0 dan 32,0 μl; Jumlah (%) sperma motil terdapatperbedaan antara dosis 0,0 μl dengan 24,0 dan 32,0 μl; Rangking keaktifan sperma terdapatperbedaan antara dosis 0,0 μl dengan 24,0 dan 32,0 μl. Pemberian rebusan akar aru berpengaruhsecara signifikan terhadap peningkatan persen sperma abnormal, dan penuruan persen sperma hidup,motil dan keaktifan.AbstractIndonesia has many species of plants that have potent of medicine, one of them cold be as antraditional contraception medicine ussually used to limit child or sterilizatiom such as root boiledwater of Caesalpinia bonduc. The aims of this study is to know the effect of root boiled water ofCaesalpinia bonduc as traditional contraception medicine on quality of sperm maturation ofepididymis of mice (Mus musculus). Mice were choosed radomly and doses gouped: 0,0 (K0 ), 16,0(K1) 24,0 (K2) and 32,0 (K3) μl/gram body weight/day. Each of group was replicated 5 times.Treatment were given by oral with using gavage needle for ten days. Surgery was carried out on firstday after completing treatments. Examination of epidymis sperm by counting number of spermproduction, percentage of motil, live, abnormal sperm, and rank of sperm motility. In order to knowthe deferences between control and treated group, was used one way anava analysis and analysedvalue for comparing between teratment group. The treatments of root boiled water of Caesalpiniabonduc have effect significantly on percentage of abnormal sperm between dose of 0,0 μl with 24,0and 32,0 μl, also between 16,0 μl with 24,0 and 32,0 μl. Percentage of live sperem is different betweendose of 0,0 μl with 16,0 , 24,0 and 32,0 μl. Percentage of motile sperm is defferent significantlybetween dose of 0,0 μl with 24,0 and 32,0 μl. Meanwhile percentage of motile rank has differencebetween dose of 0,0 μl with 24,0 and 32,0 μl. The treatment of root boiled water of Caesalpiniabonduc has effect signifiantly on increasing percentange of sperm abnormality, decreasingpercentage of life sperm, motile and rank of motile of mice sperm.


Aquaculture ◽  
2020 ◽  
Vol 520 ◽  
pp. 734996 ◽  
Author(s):  
Sylwia Judycka ◽  
Mariola Słowińska ◽  
Joanna Nynca ◽  
Ewa Liszewska ◽  
Stefan Dobosz ◽  
...  

Cryobiology ◽  
2014 ◽  
Vol 69 (3) ◽  
pp. 462-466 ◽  
Author(s):  
Filiz Kutluyer ◽  
Murathan Kayim ◽  
Fatih Öğretmen ◽  
Serhat Büyükleblebici ◽  
P. Barbaros Tuncer

2018 ◽  
Vol 30 (1) ◽  
pp. 213
Author(s):  
M. Á. Peralta-Martínez ◽  
S. R. García ◽  
M. E. Kjelland ◽  
H. González-Márquez

Rainbow trout (Oncorhynchus mykiss) sperm extender protocols can differ considerably with regards to composition of the extenders and handling. The objective of this study was to determine the effect of pH for 5 extenders on rainbow trout sperm motility and activation during storage at 5°C. Two-year-old sexually mature rainbow trout males (n = 46) weighing 1.5 to 2.5 kg were caught at Aquaculture Farm Tatakay in Jilotzingo, Estado de México, México. One semen sample per male was collected by abdominal massage. For the experiments, 100 mL of each extender (306, 512, Mounibs, Erdahl and Graham, and Hanks’ Balanced Salt Solution) was made, with pH adjusted to 6 levels (7.0, 7.2, 7.4, 7.8, 8.0, and 8.2). To evaluate pH dynamics in each extender, pH was tested daily over an 8-day storage interval. Trout sperm was added to each of the extenders in a ratio of 1:1 and motility recorded. Afterwards, each activator solution [DIA 532, saline solution (0.85%) and pond water] was added separately to a sample of the extended sperm, to initiate sperm activation. Motility was evaluated subjectively at 400× and monitored until ~99% of the sperm stopped moving (on average, in 40 s). A one-way ANOVA was used and statistical differences were set at α < 0.05. Extender 512 activated sperm motility when pH was >7.4, whereas extender 306 activated motility at every pH. Hanks’ Balanced Salt Solution activated motility at very low percentages for various pH levels, whereas Mounibs and Erdahal and Graham solutions did not activate motility for any pH tested. The 512 extender with a pH of 7 performed best (P < 0.05) as a storage solution, producing a sperm motility of 54% after activation using DIA 532. These results demonstrated the importance of evaluating effects of sperm extender pH over both short- and medium-term storage.


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