Post-testicular sperm maturation in the saltwater crocodile Crocodylus porosus: assessing the temporal acquisition of sperm motility

The role of Ca2+ in sturgeon sperm maturation and motility was investigated. Sperm from mature male sterlets (Acipenser ruthenus) were collected from the Wolffian duct and testis 24 h after hormone induction. Testicular spermatozoa (TS) were incubated in Wolffian duct seminal fluid (WDSF) for 5 min at 20°C and were designated ‘TS after IVM’ (TSM). Sperm motility was activated in media with different ion compositions, with motility parameters analysed from standard video microscopy records. To investigate the role of calcium transport in the IVM process, IVM was performed (5 min at 20°C) in the presence of 2 mM EGTA, 100 µM Verapamil or 100 µM Tetracaine. No motility was observed in the case of TS (10 mM Tris, 25 mM NaCl, 50 mM Sucr with or without the addition of 2 mM EGTA). Both incubation of TS in WDSF and supplementation of the activation medium with Ca2+ led to sperm motility. The minimal Ca2+ concentration required for motility activation of Wolffian duct spermatozoa, TS and TSM was determined (1–2 nM for Wolffian duct spermatozoa and TSM; approximately 0.6 mM for TS). Motility was obtained after the addition of verapamil to the incubation medium during IVM, whereas the addition of EGTA completely suppressed motility, implying Ca2+ involvement in sturgeon sperm maturation. Further studies into the roles of Ca2+ transport in sturgeon sperm maturation and motility are required.

Download Full-text

The assessment of sperm DNA fragmentation in the saltwater crocodile (Crocodylus porosus)

Reproduction Fertility and Development ◽

10.1071/rd15300 ◽

2017 ◽

Vol 29 (3) ◽

pp. 630 ◽

Cited By ~ 8

Author(s):

S. D. Johnston ◽

C. López-Fernández ◽

F. Arroyo ◽

J. L. Fernández ◽

J. Gosálvez

Keyword(s):

Dna Damage ◽

Dna Fragmentation ◽

Sperm Chromatin ◽

Sperm Dna Fragmentation ◽

Fragmented Dna ◽

Sperm Dna ◽

Saltwater Crocodile ◽

Crocodylus Porosus ◽

Dispersion Test ◽

Sperm Nuclei

Herein we report a method of assessing DNA fragmentation in the saltwater crocodile using the sperm chromatin dispersion test (SCDt) after including frozen–thawed spermatozoa in a microgel (Halomax; Halotech DNA, Madrid, Spain). Following controlled protein depletion, which included a reducing agent, sperm nuclei with fragmented DNA showed a homogeneous and larger halo of chromatin dispersion with a corresponding reduced nucleoid core compared with sperm with non-fragmented DNA. The presence of DNA damage was confirmed directly by incorporation of modified nucleotides using in situ nick translation (ISNT) and indirectly by studying the correlation of the SCDt with the results of DNA damage visualisation using a two-tailed comet assay (r = 0.90; P = 0.037). Results of the SCDt immediately following thawing and after 5 h incubation at 37°C in order to induce a range of DNA damage revealed individual crocodile differences in both the baseline level of DNA damage and DNA longevity.

Download Full-text

Standardized Reference Ideogram for Physical Mapping in the Saltwater Crocodile (Crocodylus porosus)

Cytogenetic and Genome Research ◽

10.1159/000293286 ◽

2009 ◽

Vol 127 (2-4) ◽

pp. 204-212 ◽

Cited By ~ 3

Author(s):

P. Dalzell ◽

L.G. Miles ◽

S.R. Isberg ◽

T.C. Glenn ◽

C. King ◽

...

Keyword(s):

Physical Mapping ◽

Saltwater Crocodile ◽

Crocodylus Porosus

Download Full-text

Outcomes of the study of intracytoplasmic sperm injection (ICSI) and sperm motility with microdissection testicular sperm extraction

Asian Journal of Andrology ◽

10.4103/aja202152 ◽

2021 ◽

Vol 0 (0) ◽

pp. 0

Author(s):

Mariko Murase ◽

Yuuka Arai ◽

Hiroe Ueno ◽

Mizuki Yamamoto ◽

Haruna Izumi ◽

...

Keyword(s):

Sperm Motility ◽

Intracytoplasmic Sperm Injection ◽

Testicular Sperm Extraction ◽

Testicular Sperm

Download Full-text

Problem crocodiles, Crocodylus porosus, at Nhulunbuy, Northern Territory: an assessment of relocation as a mangaement strategy

Wildlife Research ◽

10.1071/wr9930127 ◽

1993 ◽

Vol 20 (1) ◽

pp. 127 ◽

Cited By ~ 20

Author(s):

B Walsh ◽

PJ Whitehead

Keyword(s):

Carrying Capacity ◽

Northern Territory ◽

Effective Strategy ◽

Wet Season ◽

Rates Of Return ◽

Saltwater Crocodile ◽

Crocodylus Porosus ◽

Remote Sites ◽

Total Capture ◽

Capture Rates

Since 1986 the Conservation Commission of the Northern Territory has operated a programme to remove 'problem' saltwater crocodiles from waters in and adjoining the township of Nhulunbuy. Over a period of five years, 52 different saltwater crocodiles (44 male) were captured. Most (48) were released at remote sites designated by the traditional Aboriginal owners of the surrounding lands. Release distances from the township varied from 17 to 282 km (by coastline). Many crocodiles (47.9%), including those released at the most distant sites, returned to, and were subsequently recaptured at, the township. Total capture rates (initial captures and recaptures pooled) varied seasonally, with fewer crocodiles being caught in the cooler dry season. During the wet season capture rates remained high, but relatively fewer 'new' crocodiles were caught. Probability of recapture could not be related to distance and direction of release from the township, nor size and sex of the released crocodile. Frequency of capture of individual crocodiles (1-8 times) was also unrelated to these variables. The high rates of return indicate that relocation is unlikely to be an effective strategy for managing humancrocodile interactions, at least in areas where potential release sites already support saltwater crocodile populations near carrying capacity.

Download Full-text

PENGARUH AIR REBUSAN AKAR ARU (Caesalpinia bonduc) TERHADAP KUALITAS SPERMA EPIDIDIMIS MENCIT (Mus musculus) : DASAR PENGEMBANGAN OBAT K0NTRASEPSI TRADISIONAL BAGI LAKI-LAKI

JURNAL BIOLOGI TROPIS ◽

10.29303/jbt.v13i2.146 ◽

2013 ◽

Vol 13 (2) ◽

Author(s):

D. Setiadi Dan S. Bachri

Keyword(s):

Body Weight ◽

Sperm Motility ◽

Mus Musculus ◽

Treated Group ◽

Sperm Maturation ◽

Motile Sperm ◽

Abnormal Sperm ◽

Sperm Abnormality ◽

Boiled Water

AbstrakIndonesia memiliki banyak tumbuhan berpotensi obat salah satunya bisa dijadikan sebagai obatkontrasepsi tradisonal yang biasa digunakan untuk menjarangkan anak atau sterilisasi sepertirebusan akar Caesalpinia bonduc (Aru). Tujuan penelitian untuk mengetahui pengaruh obatkontrasepsi tradisional air rebusan akar aru terhadap kualitas pematangan sperma epididimis mencit(Mus musculus). Mencit dipilih secara acak untuk mewakili 4 kelompok dosis yaitu : 0,0 (K0), 16,0(K1) 24,0 (K2) dan 32,0 (K3) μl/gram berat badan/hari. Setiap kelompok perlakuan dilakukandengan 5 kali replikasi. Perlakuan diberikan melalui oral dengan menggunakan jarum gavage selama10 hari berturut-turut. Pembedahan dilakukan pada hari pertama setelah perlakuan selesai.Pengamatan kualitas sperma epididimis dengan perhitungan produksi sperma, % sperm motil, %sperma hidup, % sperma abnormal,dan rangking keaktifan sperma. Untuk mengetahui ada atautidaknya perbedaan yang bermakna antar perlakuan dalam kelompok dilakukan uji Anava satu arah,bila terdapat perbedaan bermakna dilanjutkan uji BNJ untuk membandingkan angka rata-rata antarkelompok perlakuan. Pemberian air rebusan akar Caesalpinea bonduc pada mencit menunjukanpengaruh perbedaan secara signifikan pada jumlah (%) sperma abnormal antara dosis 0,0 μl dengan24,0 dan 32,0 μl serta antara 16,0 μl dengan 24,0 dan 32,0 μl; Jumlah (%) sperma hidup terdapatperbedaan antara dosis 0,0 μl dengan 16,0 , 24,0 dan 32,0 μl; Jumlah (%) sperma motil terdapatperbedaan antara dosis 0,0 μl dengan 24,0 dan 32,0 μl; Rangking keaktifan sperma terdapatperbedaan antara dosis 0,0 μl dengan 24,0 dan 32,0 μl. Pemberian rebusan akar aru berpengaruhsecara signifikan terhadap peningkatan persen sperma abnormal, dan penuruan persen sperma hidup,motil dan keaktifan.AbstractIndonesia has many species of plants that have potent of medicine, one of them cold be as antraditional contraception medicine ussually used to limit child or sterilizatiom such as root boiledwater of Caesalpinia bonduc. The aims of this study is to know the effect of root boiled water ofCaesalpinia bonduc as traditional contraception medicine on quality of sperm maturation ofepididymis of mice (Mus musculus). Mice were choosed radomly and doses gouped: 0,0 (K0 ), 16,0(K1) 24,0 (K2) and 32,0 (K3) μl/gram body weight/day. Each of group was replicated 5 times.Treatment were given by oral with using gavage needle for ten days. Surgery was carried out on firstday after completing treatments. Examination of epidymis sperm by counting number of spermproduction, percentage of motil, live, abnormal sperm, and rank of sperm motility. In order to knowthe deferences between control and treated group, was used one way anava analysis and analysedvalue for comparing between teratment group. The treatments of root boiled water of Caesalpiniabonduc have effect significantly on percentage of abnormal sperm between dose of 0,0 μl with 24,0and 32,0 μl, also between 16,0 μl with 24,0 and 32,0 μl. Percentage of live sperem is different betweendose of 0,0 μl with 16,0 , 24,0 and 32,0 μl. Percentage of motile sperm is defferent significantlybetween dose of 0,0 μl with 24,0 and 32,0 μl. Meanwhile percentage of motile rank has differencebetween dose of 0,0 μl with 24,0 and 32,0 μl. The treatment of root boiled water of Caesalpiniabonduc has effect signifiantly on increasing percentange of sperm abnormality, decreasingpercentage of life sperm, motile and rank of motile of mice sperm.

Download Full-text