Analysis of candidate genes expression associated with defense responses to root and collar rot disease caused by Phytophthora capsici in peppers Capsicum annuum

Pepper (Capsicum annuum L.) is an economically significant global crop and condiment. Its yield can be severely reduced by the oomycete plant pathogen, Phytophthora capsici (P. capsici). Here, a high-density genetic map was created with a mapping panel of F2 populations obtained from 150 individuals of parental lines PI201234 and 1287 and specific-locus amplified fragment sequencing (SLAF) that was then utilized to identify loci that are related to resistance to P. capsici. The sequencing depth of the genetic map was 108.74-fold for the male parent, 126.25-fold for the female parent, and 22.73-fold for the offspring. A high-resolution genetic map consisting of 5565 markers and 12 linkage groups was generated for pepper, covering 1535.69 cM and an average marker distance of 0.28 cM. One major quantitative trait locus (QTL) for the P. capsici resistance (CQPc5.1) was identified on Chr05 that explained the observed 11.758% phenotypic variance. A total of 23 candidate genes located within the QTL CQPc5.1 interval were identified, which included the candidate gene Capana05g000595 that encodes the RPP8-like protein as well as two candidate genes Capana05g000596 and Capana05g000597 that encodes a RPP13-like protein. Quantitative reverse-transcription PCR (qRT-PCR) revealed higher expression levels of Capana05g000595, Capana05g000596, and Capana05g000597 in P. capsici resistance accessions, suggesting their association with P. capsici resistance in pepper.

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Identifying candidate genes for Phytophthora capsici resistance in pepper (Capsicum annuum) via genotyping-by-sequencing-based QTL mapping and genome-wide association study

Scientific Reports ◽

10.1038/s41598-019-46342-1 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 8

Author(s):

Muhammad Irfan Siddique ◽

Hea-Young Lee ◽

Na-Young Ro ◽

Koeun Han ◽

Jelli Venkatesh ◽

...

Keyword(s):

Qtl Mapping ◽

Association Study ◽

Candidate Genes ◽

Capsicum Annuum ◽

Genome Wide Association Study ◽

Phytophthora Capsici ◽

Genotyping By Sequencing ◽

Genome Wide Association ◽

Genome Wide

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Genetic diversity and biochemical analysis of Capsicum annuum (Bell pepper) in response to root and basal rot disease, Phytophthora capsici

Phytochemistry ◽

10.1016/j.phytochem.2021.112884 ◽

2021 ◽

Vol 190 ◽

pp. 112884

Author(s):

Leila Mohammadbagheri ◽

Mehdi Nasr-Esfahani ◽

Vahid Abdossi ◽

Davood Naderi

Keyword(s):

Genetic Diversity ◽

Capsicum Annuum ◽

Biochemical Analysis ◽

Phytophthora Capsici ◽

Bell Pepper ◽

Basal Rot ◽

Rot Disease

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Identification and Expression Analysis of Candidate Genes Associated with Defense Responses to Phytophthora capsici in Pepper Line “PI 201234”

International Journal of Molecular Sciences ◽

10.3390/ijms160511417 ◽

2015 ◽

Vol 16 (12) ◽

pp. 11417-11438 ◽

Cited By ~ 9

Author(s):

Pingyong Wang ◽

Xiaodan Liu ◽

Jinju Guo ◽

Chen Liu ◽

Nan Fu ◽

...

Keyword(s):

Candidate Genes ◽

Expression Analysis ◽

Phytophthora Capsici ◽

Defense Responses

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Management of collar rot disease in chickpea by Trichoderma species

Journal of AgriSearch ◽

10.21921/jas.v7i03.18694 ◽

2020 ◽

Vol 7 (03) ◽

Author(s):

PREM PANDEY ◽

G. C. SAGAR ◽

SUNDARMAN SHRESTHA2 ◽

HIRAKAJI MANANDHAR ◽

RITESH K. YADAV ◽

...

Keyword(s):

Growth Inhibition ◽

Mycelial Growth ◽

Sclerotium Rolfsii ◽

Maximum Growth ◽

Pot Experiment ◽

Trichoderma Spp ◽

Trichoderma Species ◽

Collar Rot ◽

Rot Disease

Nine isolates of Trichoderma spp. were isolated from different agro- ecological regions of Nepal viz; Jumla, Palpa, Chitwan, Tarahara, Banke, Illam and Salyan and screened against Sclerotium rolfsii Sacc. Adreded soil borne phytopathogen causing collar rot of chickpea in chickpea; In-vitro efficacy of nine fungal antagonist (Trichoderma spp.) against Sclerotium rolfsii were screened. Pot experiment was done to find out the effective management of S. rolfsi through Tricoderma using different methods i.e. Seed treatment, soil drenching and soil application. All the tested isolates of Trichoderma spp. were found effective on mycelial growth inhibition and sclerotial parasitization of S. rolfsii. Trichoderma isolated from Palpa district showed maximum growth inhibition (%) of pathogen periodically after 48(93.78%), 72(96.00%), 96(97.96%) and 120(100.00%) hours of inoculation. Parasitized sclerotium showed minimum sclerotial germination on agar plates. Moreover, Trichoderma species isolated from Palpa districts showed second best percent mycelial growth inhibition periodically at 72(25.00%), 120(29.16%), 168(29.16%) and 216(29.16%).In pot experiment at 40 days after sowing, Seedling height was maximum in soil drenching with 30g per 100ml of water (22.27cm) and Mortality percentage of seedlings was least or highest disease control was observed in seed treated with 109cfu/ml (0.000%).

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