Steroid-independent male sexual behavior in B6D2F2 male mice

2016 ◽  
Vol 85 ◽  
pp. 26-29
Author(s):  
Christine M. McInnis ◽  
Samitha Venu ◽  
Jin Ho Park
Keyword(s):  
Sexual Behavior ◽  
Male Mice ◽  
Male Sexual Behavior

scholarly journals Brain Aromatase and the Regulation of Sexual Activity in Male Mice

Endocrinology ◽  
2020 ◽  
Vol 161 (10) ◽  
Author(s):  
David C Brooks ◽  
John S Coon V ◽  
Cihangir M Ercan ◽  
Xia Xu ◽  
Hongxin Dong ◽  
...  
Keyword(s):  
Sexual Behavior ◽  
Sexual Activity ◽  
Adult Brain ◽  
Whole Body ◽  
Male Mice ◽  
Negative Feedback Regulation ◽  
Male Sexual Behavior ◽  
Brain Physiology ◽  
Brain Aromatase ◽  
The Brain

Abstract The biologically active estrogen estradiol has important roles in adult brain physiology and sexual behavior. A single gene, Cyp19a1, encodes aromatase, the enzyme that catalyzes the conversion of testosterone to estradiol in the testis and brain of male mice. Estradiol formation was shown to regulate sexual activity in various species, but the relative contributions to sexual behavior of estrogen that arises in the brain versus from the gonads remained unclear. To determine the role of brain aromatase in regulating male sexual activity, we generated a brain-specific aromatase knockout (bArKO) mouse. A newly generated whole-body total aromatase knockout mouse of the same genetic background served as a positive control. Here we demonstrate that local aromatase expression and estrogen production in the brain is partially required for male sexual behavior and sex hormone homeostasis. Male bArKO mice exhibited decreased sexual activity in the presence of strikingly elevated circulating testosterone. In castrated adult bArKO mice, administration of testosterone only partially restored sexual behavior; full sexual behavior, however, was achieved only when both estradiol and testosterone were administered together. Thus, aromatase in the brain is, in part, necessary for testosterone-dependent male sexual activity. We also found that brain aromatase is required for negative feedback regulation of circulating testosterone of testicular origin. Our findings suggest testosterone activates male sexual behavior in part via conversion to estradiol in the brain. These studies provide foundational evidence that sexual behavior may be modified through inhibition or enhancement of brain aromatase enzyme activity and/or utilization of selective estrogen receptor modulators.

scholarly journals Combined Effects of Oligopeptides Isolated from Panax ginseng C.A. Meyer and Ostrea gigas Thunberg on Sexual Function in Male Mice

2021 ◽  
Vol 18 (5) ◽  
pp. 2349
Author(s):  
Di Li ◽  
Jinwei Ren ◽  
Lixia He ◽  
Jingqin Sun ◽  
Peng Liu ◽  
...  
Keyword(s):  
Sexual Behavior ◽  
Sexual Function ◽  
Whey Protein ◽  
Corpus Cavernosum ◽  
Vehicle Control ◽  
Control Group ◽  
Male Mice ◽  
Vehicle Control Group ◽  
Male Sexual Behavior ◽  
Male Sexual Function

Male sexual debility affects patients’ confidence and damages the relationship between the couples and thus affects the stability of the family. This study aimed to investigate the effects of oligopeptides isolated from ginseng and oyster (GOPs and OOPs), separately and in combination, on sexual function in male mice. In the first experiment, male mice were randomly divided into five groups: vehicle control group; whey protein (125.0 mg kg−1) group; and GOPs 62.5, 125.0, and 250.0 mg kg−1 groups. In the second experiment, male mice were randomly divided into five groups: vehicle control group, whey protein (160.0 mg kg−1) group, and OOPs 80.0, 160.0, and 320.0 mg kg−1 groups. In the third experiment, male mice were randomly divided into six groups: vehicle control group, whey protein (222.5 mg kg−1) group, and GOPs + OOPs 62.5 + 160.0, 62.5 + 320.0, and 125.0 + 160.0, 125.0 + 320.0 mg kg−1 groups. Test substances were given by gavage once a day for 30 days. The sexual behavior parameters, serum nitric oxide (NO), testosterone, cyclic guanosine monophosphate (cGMP), and phosphodiesterase-5 (PDE5) concentrations were detected. We found that GOPs at 250.0 mg kg−1 improved male sexual behavior, NO, and testosterone content, whereas GOPs at 62.5 and 125.0 mg kg−1 and OOPs at 80.0, 160, and 320 mg kg−1 did not have significant effects. The combination of 62.5 mg kg−1 GOPs + 160.0 mg kg−1 OOPs and the combination of 125.0 mg kg−1 GOPs + 320.0 mg kg−1 OOPs improved male sexual behavior, serum NO, testosterone, and cGMP contents and decreased PDE5 content. The combination of 62.5 mg kg−1 GOPs and 160.0 mg kg−1 OOPs had the best effects among four combined groups. These results suggested that GOPs in combination with OOPs had the synergistic effects of enhancing male sexual function, probably via elevating serum testosterone, NO, and corpus cavernosum cGMP level and decreasing the corpus cavernosum PDE5 level. GOPs and OOPs could be novel natural agents for improving male sexual function.

scholarly journals OR09-03 Brain Aromatase Is Essential for Regulation of Sexual Activity in Male Mice

2020 ◽  
Vol 4 (Supplement_1) ◽  
Author(s):  
David C Brooks ◽  
Hong Zhao ◽  
John Coon V ◽  
C Mutlu Ercan ◽  
Hongxin Dong ◽  
...  
Keyword(s):  
Sexual Behavior ◽  
Mouse Model ◽  
Sexual Activity ◽  
Sex Hormone ◽  
Splice Acceptor Site ◽  
Male Mice ◽  
Male Sexual Behavior ◽  
Estrogen Production ◽  
Brain Aromatase ◽  
The Brain

Abstract Introduction: The biologically active form of estrogen, estradiol (E2), has important organizational roles in brain development and activational roles in adult brain physiology and behavior. It has been proposed that E2 formation in the brain might regulate sexual activity in various species. The mechanisms that link estrogen formation in the brain and sexual behavior, however, remain unclear. Aromatase is the key enzyme that catalyzes the conversion of testosterone (T) to E2 in the testis and brain of male mice. To determine the role of brain aromatase in male sexual activity, we generated a brain-specific aromatase knockout (bArKO) mouse model. Additionally, a newly generated total aromatase knockout (tArKO) mouse model served as a positive control. Methods: We generated the floxed aromatase mice (Aromfl/fl), which flanked the transcription and translation start sites and the common splice acceptor site for the upstream brain promoter I.f of the aromatase gene. We then crossed Nestin-Cre mice with Aromfl/fl mice to generate bArKO mice. Using the same Aromfl/fl mice, we bred tArKO via crossing with ZP3-Cre mice. Circulating and tissue (brain and testis) E2 levels were measured using liquid chromatography-tandem mass spectrometry. We assessed sexual activity in 12-14 week-old bArKO, tArKO and littermate control males over two 30-minute trials. The interactions were monitored and videotaped, and the videotape was scored for the sexual activity. To investigate whether the lack of estrogen production in the brain was causative for altered sexual behavior, 20 bArKO and 20 control mice were castrated at ~nine weeks of age and supplemented with exogenous sex hormone via 60-day time release pellet implantation. Results: E2 levels are significantly decreased in the brain but not the testis of bArKO mice as compared to control mice (P < 0.05, n=6-12). As expected, E2 levels in the brain and testis are significantly lower in tArKO mice compared with their WT littermates (n=6-9). Furthermore, we demonstrate that local aromatase expression and estrogen production in the brain is required for male sexual behavior and sex hormone homeostasis. Male bArKO mice exhibited significantly decreased sexual activity in the presence of strikingly elevated circulating T (n=5). In castrated adult bArKO mice, administration of E2 together with T restored maximum sexual behavior (n=5). Thus, aromatase in the brain is necessary for T-dependent male sexual activity. We also found that brain aromatase is required for negative feedback regulation of circulating T of testicular origin. Conclusion: Our findings suggest T activates male sexual behavior in part via conversion to E2 in the brain and provide the foundation for inhibition or enhancement of brain aromatase enzyme activity and/or utilization of selective estrogen receptor modulators in modifying sexual behavior. DCB and HZ contributed equally to this work.

Peripubertal gonadal steroids are necessary for steroid-independent male sexual behavior in castrated B6D2F1 male mice

2019 ◽  
Vol 113 ◽  
pp. 38-46 ◽  
Author(s):  
Jay Scott Templin ◽  
Joshua C. Wyrosdic ◽  
Caroline D. David ◽  
Brianna N. Wyrosdic ◽  
Hannah E. Lapp ◽  
...  
Keyword(s):  
Sexual Behavior ◽  
Gonadal Steroids ◽  
Male Mice ◽  
Male Sexual Behavior

1625: Role of Estrogen Receptor β in the Regulation of Sexual Behavior in Male Mice

2004 ◽  
Vol 171 (4S) ◽  
pp. 429-429
Author(s):  
Masayoshi Nomura ◽  
Naohiro Fujimoto ◽  
Donald W. Pfaff ◽  
Sonoko Ogawa ◽  
Tetsuro Matsumoto
Keyword(s):  
Estrogen Receptor ◽  
Sexual Behavior ◽  
Estrogen Receptor Β ◽  
Male Mice

scholarly journals Effects of pomegranate juice on the sexual behavior, fertility and protective activity against aluminum exposure in male mice

2020 ◽  
Vol 32 (6) ◽  
pp. 2688-2695
Author(s):  
Mohsen Ghaleb Al-Mutary ◽  
Gasem Mohammad Abu-Taweel
Keyword(s):  
Sexual Behavior ◽  
Pomegranate Juice ◽  
Protective Activity ◽  
Male Mice ◽  
Aluminum Exposure

Maternal treatment with picrotoxin in late pregnancy improved female sexual behavior but did not alter male sexual behavior of offspring

2013 ◽  
Vol 24 (4) ◽  
pp. 282-290 ◽  
Author(s):  
Maria M. Bernardi ◽  
Kayne K. Scanzerla ◽  
Mayra Chamlian ◽  
Elizabeth Teodorov ◽  
Luciano F. Felicio
Keyword(s):  
Sexual Behavior ◽  
Late Pregnancy ◽  
Female Sexual Behavior ◽  
Male Sexual Behavior ◽  
Maternal Treatment

Medial preoptic connections with the midbrain tegmentum are essential for male sexual behavior

1984 ◽  
Vol 32 (1) ◽  
pp. 79-84 ◽  
Author(s):  
Nancy L. Brackett ◽  
David A. Edwards
Keyword(s):  
Sexual Behavior ◽  
Male Sexual Behavior ◽  
Midbrain Tegmentum

scholarly journals Induction of Male Sexual Behavior in the Rat by 7α-Methyl-19-Nortestosterone, an Androgen that does not Undergo 5α-Reduction1

1993 ◽  
Vol 49 (3) ◽  
pp. 577-581 ◽  
Author(s):  
Gabriela Moralí ◽  
Ana Elena Lemus ◽  
Raul Munguía ◽  
Marcela Arteaga ◽  
Gregorio Pérez-Palacios ◽  
...  
Keyword(s):  
Sexual Behavior ◽  
Male Sexual Behavior
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