scholarly journals PAQMAN: Protein-nucleic acid affinity quantification by MAss spectrometry in nuclear extracts

Methods ◽  
2020 ◽  
Vol 184 ◽  
pp. 70-77 ◽  
Author(s):  
Cathrin Gräwe ◽  
Matthew M. Makowski ◽  
Michiel Vermeulen
Keyword(s):  
Mass Spectrometry ◽  
Nucleic Acid ◽  
Nuclear Extracts ◽  
Protein Nucleic Acid ◽  
Affinity Quantification

scholarly journals Bifunctional cross-linking approaches for mass spectrometry-based investigation of nucleic acids and protein-nucleic acid assemblies

Methods ◽  
2018 ◽  
Vol 144 ◽  
pp. 64-78 ◽  
Author(s):  
M. Scalabrin ◽  
S.M. Dixit ◽  
M.M. Makshood ◽  
C.E. Krzemien ◽  
Daniele Fabris
Keyword(s):  
Mass Spectrometry ◽  
Nucleic Acid ◽  
Nucleic Acids ◽  
Cross Linking ◽  
Protein Nucleic Acid

Snapshot blotting: The transfer of nucleic acids and nucleoprotein complexes from electrophoresis gels to EM grids

1992 ◽  
Vol 50 (1) ◽  
pp. 762-763
Author(s):  
Stephen D. Jett
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Nucleic Acid Binding ◽  
Mobility Shift ◽  
Carbon Coated ◽  
Nucleoprotein Complexes ◽  
Mobility Shift Assay ◽  
Protein Nucleic Acid ◽  
Gel Mobility Shift ◽  
Nucleic Acid Interactions

The electrophoresis gel mobility shift assay is a popular method for the study of protein-nucleic acid interactions. The binding of proteins to DNA is characterized by a reduction in the electrophoretic mobility of the nucleic acid. Binding affinity, stoichiometry, and kinetics can be obtained from such assays; however, it is often desirable to image the various species in the gel bands using TEM. Present methods for isolation of nucleoproteins from gel bands are inefficient and often destroy the native structure of the complexes. We have developed a technique, called “snapshot blotting,” by which nucleic acids and nucleoprotein complexes in electrophoresis gels can be electrophoretically transferred directly onto carbon-coated grids for TEM imaging.

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