Detection and identification of trypanosomes by polymerase chain reaction in wild tsetse flies in Cameroon

Acta Tropica ◽  
1998 ◽  
Vol 70 (1) ◽  
pp. 109-117 ◽  
Author(s):  
I Morlais ◽  
P Grebaut ◽  
J.M Bodo ◽  
S Djoha ◽  
G Cuny ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Tsetse Flies ◽  
Chain Reaction ◽  
Detection And Identification ◽  
Polymerase Chain

Detection and identification of Candida spp. in human serum by LightCycler® real-time polymerase chain reaction

2008 ◽  
Vol 60 (3) ◽  
pp. 263-271 ◽  
Author(s):  
Catherine Dunyach ◽  
Sébastien Bertout ◽  
Cécile Phelipeau ◽  
Pascal Drakulovski ◽  
Jacques Reynes ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Human Serum ◽  
Real Time ◽  
Chain Reaction ◽  
Candida Spp ◽  
Detection And Identification ◽  
Polymerase Chain

scholarly journals Current and emerging assays for Francisella tularensis detection: a review

2008 ◽  
Vol 53 (No. 11) ◽  
pp. 585-594 ◽  
Author(s):  
M. Pohanka ◽  
M. Hubalek ◽  
V. Neubauerova ◽  
A. Macela ◽  
M. Faldyna ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Flow Cytometry ◽  
Polymerase Chain Reaction ◽  
Francisella Tularensis ◽  
Practical Importance ◽  
Pathogenic Bacterium ◽  
Chain Reaction ◽  
Detection And Identification ◽  
The Future ◽  
Polymerase Chain

This paper presents an overview of methods for detection and identification of the pathogenic bacterium <I>Francisella tularensis</I> such as cultivation tests, enzyme-linked immunosorbent assays, flow cytometry, polymerase chain reaction, immunosensor, microarray, mass spectrometry, and chromatography. Included references are chosen according to their practical importance or perspectives for the future.

Detection of trypanosome infections in the saliva of tsetse flies and buffy-coat samples from antigenaemic but aparasitaemic cattle

Parasitology ◽  
1994 ◽  
Vol 108 (3) ◽  
pp. 313-322 ◽  
Author(s):  
P. A. O. Majiwa ◽  
R. Thatthi ◽  
S. K. Moloo ◽  
J. H. P. Nyeko ◽  
L. H. Otieno ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Buffy Coat ◽  
Dna Probes ◽  
Tsetse Flies ◽  
Chain Reaction ◽  
African Trypanosomes ◽  
Polymerase Chain

SUMMARYRelatively simple protocols employing non-radioactive DNA probes have been used for the detection of African trypanosomes in the blood of mammalian hosts and the saliva of live tsetse flies. In combination with the polymerase chain reaction (PCR), the protocols revealed trypanosomes in buffy-coat samples from antigenaemic but aparasitaemic cattle and in the saliva of live, infected tsetse flies. Furthermore, the protocols were used to demonstrate concurrent natural infections of single tsetse flies with different species of African trypanosomes.

Detection and identification of Clavibacter michiganensis subsp. sepedonicus and Clavibacter michiganensis subsp. michiganensis by nonradioactive hybridization, polymerase chain reaction, and restriction enzyme analysis

1994 ◽  
Vol 40 (12) ◽  
pp. 1007-1018 ◽  
Author(s):  
J. L. W. Rademaker ◽  
J. D. Janse
Keyword(s):  
Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Product ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Bacterial Strains ◽  
Clavibacter Michiganensis ◽  
Chain Reaction ◽  
Clavibacter Michiganensis Subsp ◽  
Detection And Identification ◽  
Polymerase Chain

To develop a rapid and reliable detection and identification method for Clavibacter michiganensis subsp. sepedonicus and C. michiganensis subsp. michiganensis, two biotinylated probes and derived primer sets were evaluated for specificity using a large number of bacterial strains. Detection in dot blot analysis using the Diagen probe against C. michiganensis subsp. sepedonicus was possible with all 32 C. michiganensis subsp. sepedonicus strains tested. Cross-hybridization occurred with all nine C. michiganensis subsp. insidiosus strains tested. No hybridization occurred with any of 54 other related and unrelated bacterial strains including C. michiganensis subsp. michiganensis, C. michiganensis subsp. nebraskensis, C. michiganensis subsp. tessellarius, C. iranicus, C. rathayi, and C. tritici and potato saprophytes. Hybridization of the MIC 1 probe against C. michiganensis subsp. michiganensis was obtained with 22 out of 24 C. michiganensis subsp. michiganensis strains. A weak hybridization signal occurred only with two strains of C. michiganensis subsp. insidiosns. No hybridization occurred with any of the 71 other related and unrelated bacterial strains tested including tomato saprophytes. Restriction fragment length polymorphisms detected with the Diagen probe allowed differentiation between C. michiganensis subsp. sepedonicus and the related C. michiganensis subsp. insidiosus. Restriction fragment length polymorphism analysis using the MIC 1 probe and BamH1 showed at least two groups of patterns within C. michiganensis subsp. michiganensis. By using a primer set derived from the Diagen probe, a DNA sequence could be amplified with all C. michiganensis subsp. sepedonicus strains tested. Only the nontarget organism C. michiganensis subsp. insidiosus yielded a similar polymerase chain reaction product. Restriction enzyme analysis of the polymerase chain reaction product enabled rapid distinction between the subspecies. With a CMM primer set derived from the MIC 1 probe a DNA sequence was amplified from the same 22 out of 24 C. michiganensis subsp. michiganensis strains that showed hybridization with the MIC 1 probe. The polymerase chain reaction product could be verified by restriction enzyme analysis. The Diagen and MIC 1 probes and derived primer sets were shown to be useful for the detection and identification of C. michiganensis subsp. sepedonicus and C. michiganensis subsp. michiganensis. The MIC 1 probe, however, failed to detect two strains of the latter subspecies.Key words: biotin, PCR, REA, potato bacterial ring rot, bacterial canker of tomato, RFLP, Clavibacter michiganensis subsp. insidiosus.

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