Detection of trypanosome infections in the saliva of tsetse flies and buffy-coat samples from antigenaemic but aparasitaemic cattle

Parasitology ◽  
1994 ◽  
Vol 108 (3) ◽  
pp. 313-322 ◽  
Author(s):  
P. A. O. Majiwa ◽  
R. Thatthi ◽  
S. K. Moloo ◽  
J. H. P. Nyeko ◽  
L. H. Otieno ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Buffy Coat ◽  
Dna Probes ◽  
Tsetse Flies ◽  
Chain Reaction ◽  
African Trypanosomes ◽  
Polymerase Chain

SUMMARYRelatively simple protocols employing non-radioactive DNA probes have been used for the detection of African trypanosomes in the blood of mammalian hosts and the saliva of live tsetse flies. In combination with the polymerase chain reaction (PCR), the protocols revealed trypanosomes in buffy-coat samples from antigenaemic but aparasitaemic cattle and in the saliva of live, infected tsetse flies. Furthermore, the protocols were used to demonstrate concurrent natural infections of single tsetse flies with different species of African trypanosomes.

Systemic Mycobacterium avium Infection in a Dog Diagnosed by Polymerase Chain Reaction Analysis of Buffy Coat

2005 ◽  
Vol 41 (2) ◽  
pp. 128-132 ◽  
Author(s):  
James F. Naughton ◽  
Katrina L. Mealey ◽  
K. Jane Wardrop ◽  
J. Lindsay Oaks ◽  
Daniel S. Bradway
Keyword(s):  
Polymerase Chain Reaction ◽  
Clinical Signs ◽  
Mycobacterial Infection ◽  
Polymerase Chain Reaction Analysis ◽  
Buffy Coat ◽  
Pcr Analysis ◽  
Chain Reaction ◽  
The Public ◽  
Avium Infection ◽  
Polymerase Chain

Dogs may be infected by Mycobacterium (M.) tuberculosis, M. bovis, and M. avium complex, and the clinical signs associated with each of these infections may be indistinguishable. Rapid speciation of the infecting organism is desirable because of the public health concerns associated with M. bovis and M. tuberculosis infections. A mycobacterial infection was suspected in the dog of this report based on acid-fast staining of organisms in macrophages obtained from liver aspirates and buffy-coat preparations. Polymerase chain reaction (PCR) analysis of a buffy-coat preparation identified M. avium.

Detection and identification of trypanosomes by polymerase chain reaction in wild tsetse flies in Cameroon

Acta Tropica ◽  
1998 ◽  
Vol 70 (1) ◽  
pp. 109-117 ◽  
Author(s):  
I Morlais ◽  
P Grebaut ◽  
J.M Bodo ◽  
S Djoha ◽  
G Cuny ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Tsetse Flies ◽  
Chain Reaction ◽  
Detection And Identification ◽  
Polymerase Chain

scholarly journals Comparative suitability of the feathers, peripheral blood and spleen tissue samples of chickens for the detection of Marek’s disease virus by polymerase chain reaction

2019 ◽  
Vol 35 (1-2) ◽  
pp. 1-6
Author(s):  
MW Rahman ◽  
M Nooruzzaman ◽  
US Suma ◽  
EH Chowdhury ◽  
MR Islam
Keyword(s):  
Polymerase Chain Reaction ◽  
Disease Virus ◽  
Peripheral Blood ◽  
Buffy Coat ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Chain Reaction ◽  
Polymerase Chain

A total of 24 clinical specimens (10 feathers, 8 peripheral bloods and 6 spleens) were collected from 10 suspected outbreaks of Marek’s disease (MD) from Mymensingh, Tangail, Gazipur and Pabna districts of Bangladesh. A polymerase chain reaction (PCR) protocol originally described by Silva (1992) was adopted to detect Marek’s disease virus (MDV) genome in these specimens. All the tested peripheral blood buffy coat samples (100%) were positive for MDV in PCR, while 70% of feather samples and 66.6% of spleen samples were positive. A band of 317 bp size was found in all positive samples. A few samples also yielded additional bands of 185 bp size and/or multiple bands of larger than 317 bp size, indicating the presence of both virulent MDV and the vaccine virus. The study suggests that peripheral blood and feathers from live birds, and feathers from dead birds are the samples of choice for the detection of MDV by PCR. The Bangladesh Veterinarian (2018) 35(1&2): 1-6

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