scholarly journals Charge Translocation by the Na+/K+-ATPase Investigated on Solid Supported Membranes: Cytoplasmic Cation Binding and Release

1999 ◽  
Vol 76 (2) ◽  
pp. 827-836 ◽  
Author(s):  
J. Pintschovius ◽  
K. Fendler ◽  
E. Bamberg
Keyword(s):  
Cation Binding ◽  
Supported Membranes ◽  
Charge Translocation ◽  
Solid Supported Membranes

Phosphorylation of C-terminal polycystin-2 influences the interaction with PIGEA14: A QCM study based on solid supported membranes

2013 ◽  
Vol 437 (4) ◽  
pp. 532-537 ◽  
Author(s):  
Daniela Morick ◽  
Michaela Schatz ◽  
Raphael Hubrich ◽  
Helen Hoffmeister ◽  
Anya Krefft ◽  
...  
Keyword(s):  
Supported Membranes ◽  
Solid Supported Membranes

scholarly journals Measuring Ion Channels on Solid Supported Membranes

2009 ◽  
Vol 97 (1) ◽  
pp. 388-396 ◽  
Author(s):  
Patrick Schulz ◽  
Benjamin Dueck ◽  
Alexandre Mourot ◽  
Lina Hatahet ◽  
Klaus Fendler
Keyword(s):  
Ion Channels ◽  
Supported Membranes ◽  
Solid Supported Membranes

Robust Electrophysiological Assays using Solid Supported Membranes: the Organic Cation Transporter OCT2

2011 ◽  
Vol 64 (1) ◽  
pp. 31 ◽  
Author(s):  
Olga Gaiko ◽  
Ingo Janausch ◽  
Sven Geibel ◽  
Henning Vollert ◽  
Petra Arndt ◽  
...  
Keyword(s):  
Cell Lines ◽  
Organic Cation ◽  
Chinese Hamster ◽  
Organic Cation Transporter ◽  
Supported Membranes ◽  
Cho Cell ◽  
Drug Candidates ◽  
Substrate Affinities ◽  
Solid Supported Membranes

An electrophysiological assay platform based on solid supported membranes (SSM) for the organic cation transporter (OCT) is presented. Stable Chinese hamster ovary (CHO) cell lines overexpressing the human (hOCT2) and rat transporters (rOCT2) were generated and validated. Membrane preparations from the cell lines were investigated using SSM-based electrophysiology. Baculovirus transfected insect cells (HighFive and Mimic Sf9) were also tested with the same assay but yielded less than optimal results. The assays were validated by the determination of substrate affinities and inhibition by standard inhibitors. The study demonstrates the suitability of the SSM-based electrophysiological OCT assay for rapid and automatic screening of drug candidates.

Transporter Assays Using Solid Supported Membranes: A Novel Screening Platform for Drug Discovery

2006 ◽  
Vol 4 (5) ◽  
pp. 575-582 ◽  
Author(s):  
Bela Kelety ◽  
Kerstin Diekert ◽  
Joanna Tobien ◽  
Natalie Watzke ◽  
Wolfgang Dörner ◽  
...  
Keyword(s):  
Drug Discovery ◽  
Supported Membranes ◽  
Screening Platform ◽  
Solid Supported Membranes
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