An improved filter-disc assay for 3H-DNA and serum deoxyribonucleases

1977 ◽  
Vol 10 ◽  
pp. 188-190 ◽  
Author(s):  
Richard Albert Cox ◽  
Muharrem Gokcen
Keyword(s):  
Filter Disc ◽  
Disc Assay

Mass sensing by filter disc resonance: A progress report

1998 ◽  
Vol 29 ◽  
pp. S961-S962
Author(s):  
P. Lilienfeld ◽  
R. Steg
Keyword(s):  
Progress Report ◽  
Mass Sensing ◽  
Filter Disc

Activity of myxin against Ceratocystis ulmi

1969 ◽  
Vol 15 (1) ◽  
pp. 133-135 ◽  
Author(s):  
E. A. Peterson
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Liquid Culture ◽  
Inhibitory Concentration ◽  
In Vitro Tests ◽  
Strong Inhibition ◽  
Solid Media ◽  
Highly Sensitive ◽  
Paper Disc ◽  
Disc Assay

Eight strains of Ceratocystis ulmi originating from different locations and host species were found to be highly sensitive to the antibiotic myxin in in vitro tests. By paper disc assay, amounts as low as 0.5–1.0 μg caused strong inhibition of the fungus on solid media. The minimum inhibitory concentration in liquid culture was 0.2 μg/ml and levels of antibiotic above this concentration proved to be fungicidal.

scholarly journals Evaluation of powdery mildew resistance in various melon (Cucumis melo L.) genotypes

2018 ◽  
Vol 10 (4) ◽  
pp. 279-284
Author(s):  
Zh. Ivanova ◽  
K. Vasileva ◽  
N. Velkov ◽  
S. Grozeva
Keyword(s):  
Powdery Mildew ◽  
Genetic Resistance ◽  
Leaf Disc ◽  
Breeding Program ◽  
Vegetable Crops ◽  
Plant Materials ◽  
South Central ◽  
Leaf Disc Assay ◽  
Disc Assay

Abstract. Powdery mildew, caused by Podosphaera xanthii and Golovinomyces cichoracearum, is an economically important disease in melon worldwide. Genetic resistance is one of the most suitable strategies to control powdery mildew. During the last few years several races of the pathogens have been reported. The need to develop resistant varieties is a challenge for each breeding program. Leaf disc assay was used in phytopathology and breeding programs as a rapid and reliable method for evaluation of disease resistance in a large number of plant materials. The purpose of this study was to establish species and races of powdery mildew in Plovdiv region, South Central Bulgaria; to develop a suitable system of pathogen isolation and cultivation; to determine the resistance levels in different melon genotypes available in Maritsa Vegetable Crops Research Institute (MVCRI) - Plovdiv collection by the leaf disc assay. Fifty-three melon genotypes, including lines, varieties, hybrids and ten differential lines were tested. The data showed that causal agent of powdery mildew was race 1 of P. xanthii in Plovdiv region. Our experimental results indicated that for the long-term storage of powdery mildew it is preferable to keep a whole plant under in vitro conditions. This allows the preservation of powdery mildew for two months before transferring on a new tissue. Thirty-four of the tested melon genotypes reacted as immune or resistant and nineteen as susceptible. Resistant melon genotypes are a suitable source in initiating a new breeding program aimed to increase resistance to powdery mildew.

Bacillus Stearothermophilus Disc Assay: A Review

1995 ◽  
Vol 78 (6) ◽  
pp. 1408-1415
Author(s):  
Stanley E Katz ◽  
Marie Siewierski
Keyword(s):  
Bacillus Stearothermophilus ◽  
Assay Development ◽  
The Future ◽  
Development Data ◽  
History Of ◽  
Disc Assay

Abstract The literature on the Bacillus stearothermophilus disc assay was reviewed and evaluated. The history of the assay development; data on applicability, sensitivity, interferences, and cowside screening; potential for the future; and limitations are presented and discussed.

scholarly journals A detached petal disc assay and virus-induced gene silencing facilitate the study of Botrytis cinerea resistance in rose flowers

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Xiaoqian Cao ◽  
Huijun Yan ◽  
Xintong Liu ◽  
Dandan Li ◽  
Mengjie Sui ◽  
...  
Keyword(s):  
Jasmonic Acid ◽  
Gene Silencing ◽  
Botrytis Cinerea ◽  
High Throughput ◽  
Regulatory Protein ◽  
Gray Mold ◽  
Virus Induced Gene Silencing ◽  
Potential Benefits ◽  
Rose Petals ◽  
Disc Assay

AbstractFresh-cut roses (Rosa hybrida) are one of the most important ornamental crops worldwide, with annual trade in the billions of dollars. Gray mold disease caused by the pathogen Botrytis cinerea is the most serious fungal threat to cut roses, causing extensive postharvest losses. In this study, we optimized a detached petal disc assay (DPDA) for artificial B. cinerea inoculation and quantification of disease symptoms in rose petals. Furthermore, as the identification of rose genes involved in B. cinerea resistance could provide useful genetic and genomic resources, we devised a virus-induced gene silencing (VIGS) procedure for the functional analysis of B. cinerea resistance genes in rose petals. We used RhPR10.1 as a reporter of silencing efficiency and found that the rose cultivar ‘Samantha’ showed the greatest decrease in RhPR10.1 expression among the cultivars tested. To determine whether jasmonic acid and ethylene are required for B. cinerea resistance in rose petals, we used VIGS to silence the expression of RhLOX5 and RhEIN3 (encoding a jasmonic acid biosynthesis pathway protein and an ethylene regulatory protein, respectively) and found that petal susceptibility to B. cinerea was affected. Finally, a VIGS screen of B. cinerea-induced rose transcription factors demonstrated the potential benefits of this method for the high-throughput identification of gene function in B. cinerea resistance. Collectively, our data show that the combination of the DPDA and VIGS is a reliable and high-throughput method for studying B. cinerea resistance in rose.

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