Transient-state and steady-state kinetics of the oxidation of aliphatic and aromatic thiols by horseradish peroxidase

FEBS Letters ◽  
1997 ◽  
Vol 411 (2-3) ◽  
pp. 269-274 ◽  
Author(s):  
U. Burner ◽  
C. Obinger
Keyword(s):  
Steady State ◽  
Horseradish Peroxidase ◽  
Transient State ◽  
Steady State Kinetics ◽  
Aromatic Thiols ◽  
Kinetics Of

scholarly journals Studies on alkaline phosphatase. Transientstate and steady-state kinetics of Escherichia coli alkaline phosphatase

1969 ◽  
Vol 111 (2) ◽  
pp. 187-194 ◽  
Author(s):  
H N Fernley ◽  
P. G. Walker
Keyword(s):  
Escherichia Coli ◽  
Alkaline Phosphatase ◽  
Steady State ◽  
Substrate Concentration ◽  
Marked Effect ◽  
Binding Constants ◽  
Transient State ◽  
Steady State Kinetics ◽  
Ph Range ◽  
Kinetics Of

1. The transient-state and steady-state phases of the reaction between Escherichia coli alkaline phosphatase and 4-methylumbelliferyl phosphate were investigated by using a fluorimetric stopped-flow technique. 2. At low substrate concentration (5μm) in the pH range 3·8–6·3 there was an initial rapid liberation of up to 1mole of 4-methylumbelliferone/mole of enzyme. 3. At very low substrate concentration (0·1μm) in the pH range 4·9–5·9 an initial lag in 4-methylumbelliferone production was observed, from which values for k+1 and k−1 could be obtained. 4. The pH profiles for the rates of phosphorylation and dephosphorylation are quite different, and it is postulated that an ionizing group which determines the conformation during the phosphorylation step is not involved in the dephosphorylation step. 5. The binding constants for substrate and Pi are similar throughout the pH range 4–8. The ionization of substrate or Pi appeared to have no marked effect on the binding.

Steady-state kinetics of valproic acid in epileptic patients

1978 ◽  
Vol 24 (3) ◽  
pp. 324-332 ◽  
Author(s):  
J. Bruni ◽  
B. J. Wilder ◽  
L. J. Willmore ◽  
R. J. Perchalski ◽  
H. J. Villarreal
Keyword(s):  
Valproic Acid ◽  
Steady State ◽  
Steady State Kinetics ◽  
Epileptic Patients ◽  
Kinetics Of

Steady-state kinetics of bezafibrate retard in hyperlipidemic geriatric patients

1988 ◽  
Vol 66 (6) ◽  
pp. 250-256 ◽  
Author(s):  
G. Neugebauer ◽  
D. Platt ◽  
T. Vömel ◽  
W. Lösch
Keyword(s):  
Steady State ◽  
Geriatric Patients ◽  
Steady State Kinetics ◽  
Kinetics Of
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