Sa2043 Inherent Single Nucleotide Variants in the TBX21 Gene As Novel Prognostic Markers in Patients With Pancreatic Ductal Adenocarcinoma

Abstract Background: To explore significance of CA19-9, D-dimer with TNFAIP3 (A20) protein and evaluate its prognostic signiﬁcance in patients suffering from pancreatic ductal adenocarcinoma (PDAC). Methods: 148 patients suffering from pancreatic ductal adenocarcinoma in Northern Jiangsu People’s Hospital affiliated to Yangzhou University between January 2012 to December 2016 were studied. Cutoff values of prognostic factors were predicted by Receiver operating characteristic curve (ROC curve). Kaplan-Meier method was used to describe survival curve of patients. Univariate and multivariate regression analyses were used to analyze prognostic factors of patients. Results: The recommended cutoff values of the neutrophil-lymphocyte rate (NLR), platelet-lymphocyte rate (PLR), CA19-9 and D-dimer were 2.04 (sensitivity, 0.59; specificity, 0.9; area under the ROC curve (AUC), 0.749; P<0.001), 52.94 (sensitivity, 0.73; specificity, 0.95; AUC, 0.829; P<0.001), 176.66U/ml (sensitivity, 0.7; specificity, 0.9; AUC, 0.794; P<0.001) and 1.18mg/L (sensitivity, 0.82; specificity, 0.9; AUC, 0.845; P<0.001) respectively. The expression of CA19-9 in serum was related with lymph node metastasis (P = 0.010), tumor lymph node metastasis (TNM) stage (P <0.001) and survival ratio (P <0.001). The D-dimer was positively related with differentiation grade (P=0.014), tumor size (P=0.045), TNM stage (P=0.006) and survival ratio (P<0.001). A20 was positively related with differentiation grade (P<0.001), BMI (P<0.001), TNM stage (P=0.024) and survival ratio (P<0.001). The Kaplan-Meier curves showed that the patients with pancreatic ductal adenocarcinoma had a significant difference in the expression of CA19-9 group, expression of D-dimer group and expression of A20 (P<0.05). CA19-9, D-dimer, TNM stage, differentiation grade and A20 were independent prognostic markers for patients sufferingfrom pancreatic ductal adenocarcinoma by univariate and COX multivariate analyses. Conclusions: CA19-9, D-dimer and A20 were independent prognostic markers for pancreatic ductal adenocarcinoma patients.

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The effect of neuroendocrine differentiation on the survival of patients diagnosed with pancreatic ductal adenocarcinoma

Experimental and Clinical Gastroenterology ◽

10.31146/1682-8658-ecg-184-12-38-44 ◽

2020 ◽

pp. 38-44

Author(s):

O. I. Kit ◽

E. M. Franciyanc ◽

I. S. Derizhanova ◽

N. S. Karnaukhov ◽

M. A. Kuznetsova ◽

...

Keyword(s):

Pancreatic Ductal Adenocarcinoma ◽

Chromogranin A ◽

Pancreatic Tumor ◽

Prognostic Markers ◽

Patient Survival ◽

Immunohistochemical Study ◽

Neuroendocrine Differentiation ◽

Ductal Adenocarcinoma ◽

Ki 67 ◽

Cancer Research Institute

Purpose of the study. Determine the frequency of MiNeN among pancreatic carcinomas and analyze the survival rate of patients depending on the percentage of cells with neuroendocrine differentiation in the tumor.Materials and methods. The current study included 31 patients with a pancreatic tumor who received surgical treatment at the Rostov Cancer Research Institute. An immunohistochemical study was conducted on biomarkers of chromogranin A, synaptophysin, and ki-67 for these patients. Based on the data obtained, 4 groups for neuroendocrine differentiation were identified.Results. The direct effect of neuroendocrine differentiation on the survival of patients with histologically confirmed pancreatic ductal adenocarcinoma has been proven. Among the sample of 31 patients, neuroendocrine differentiation was revealed in 24 cases (77%), of which 3 cases of MiNeN (10.3%) were detected. It is also proven relationship between neuroendocrine and patient survival, where an increase percent of positive cells in tumors (chromogranin A or synaptophysin) means a better prognosis. Chromogranin A is a more significant predictor of survival compared to synaptophysin. The largest difference in survival was between negative expression of chromogranin A and the presence of more than 1% positive cells in the tumor.Conclusion. We supposed that it is necessary to use neuroendocrine markers (chromogranin A and synaptophysin) in the diagnosis of ductal adenocarcinomas, even without histological signs of neuroendocrine differentiation. This will allow for a larger amount of data to determine their significance as prognostic markers.

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Abstract 4994: Association of protein inhibitor of activated STAT protein 4 with the development of pancreatic ductal adenocarcinoma identified by chromosomal analysis from single nucleotide polymorphism arrays

10.1158/1538-7445.am10-4994 ◽

2010 ◽

Author(s):

Wenwen Chien ◽

Norihiko Kawamata ◽

Kazu Okemoto ◽

Seishi Ogawa ◽

H. Phillip Koeffler

Keyword(s):

Single Nucleotide Polymorphism ◽

Pancreatic Ductal Adenocarcinoma ◽

Chromosomal Analysis ◽

Ductal Adenocarcinoma ◽

Protein Inhibitor ◽

Nucleotide Polymorphism ◽

Single Nucleotide

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Preoperative carbohydrate antigen 19‐9 and standard uptake value of positron emission tomography‐computed tomography as prognostic markers in patients with pancreatic ductal adenocarcinoma

Journal of Hepato-Biliary-Pancreatic Sciences ◽

10.1002/jhbp.845 ◽

2020 ◽

Author(s):

Dokyoon Moon ◽

Hongbeom Kim ◽

Youngmin Han ◽

Yoonhyeong Byun ◽

Yoojin Choi ◽

...

Keyword(s):

Computed Tomography ◽

Positron Emission Tomography ◽

Pancreatic Ductal Adenocarcinoma ◽

Prognostic Markers ◽

Standard Uptake Value ◽

Carbohydrate Antigen ◽

Emission Tomography ◽

Ductal Adenocarcinoma ◽

Positron Emission

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A single nucleotide polymorphism in the CD44 gene affects survival of pancreatic ductal adenocarcinoma

HPB ◽

10.1016/j.hpb.2016.03.104 ◽

2016 ◽

Vol 18 ◽

pp. e422

Author(s):

L.F. Grochola ◽

G. Stracquadanio ◽

R. Graf ◽

B. Vrugt ◽

S. Breitenstein ◽

...

Keyword(s):

Single Nucleotide Polymorphism ◽

Pancreatic Ductal Adenocarcinoma ◽

Ductal Adenocarcinoma ◽

Nucleotide Polymorphism ◽

Single Nucleotide

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In Silico Transcriptomic Analysis of the Chloride Intracellular Channels (CLIC) Interactome Identifies a Molecular Panel of Seven Prognostic Markers in Patients with Pancreatic Ductal Adenocarcinoma

Current Genomics ◽

10.2174/1389202921666200316115631 ◽

2020 ◽

Vol 21 (2) ◽

pp. 119-127

Author(s):

Dimitrios E. Magouliotis ◽

Nikos Sakellaridis ◽

Konstantinos Dimas ◽

Vasiliki S. Tasiopoulou ◽

Konstantina A. Svokos ◽

...

Keyword(s):

Gene Family ◽

Pancreatic Ductal Adenocarcinoma ◽

In Silico ◽

Prognostic Markers ◽

Gene Set Enrichment Analysis ◽

Molecular Signature ◽

Ductal Adenocarcinoma ◽

Prostaglandin E ◽

Induced Differentiation ◽

Intracellular Channel

Background: Pancreatic ductal adenocarcinoma (PDAC) is associated with poor prognosis. In this context, the identification of biomarkers regarding the PDAC diagnosis, monitoring, and prognosis is crucial. Objectives: The purpose of the current study was to investigate the differential gene expression profile of the chloride intracellular channel (CLIC) gene family network in patients with PDAC, in order to suggest novel biomarkers. Methods: In silico techniques were used to construct the interactome of the CLIC gene family, identify the differentially expressed genes (DEGs) in PDAC as compared to healthy controls, and evaluate their potential prognostic role. Results: Transcriptomic data of three microarray datasets were included, incorporating 114 tumor and 59 normal pancreatic samples. Twenty DEGs were identified; eight were up-regulated and twelve were downregulated. A molecular signature of seven genes (Chloride Intracellular Channel 1 – CLIC1; Chloride Intracellular Channel 3 – CLIC3; Chloride Intracellular Channel 4 – CLIC4; Ganglioside Induced Differentiation Associated Protein 1 – GDAP1; Ganglioside Induced Differentiation Associated Protein 1 Like 1 – GDAP1L1; Glutathione S-Transferase Pi 1 - GSTP1; Prostaglandin E Synthase 2 – PTGES2) were identified as prognostic markers associated with overall survival. Positive correlations were reported regarding the expression of CLIC1-CLIC3, CLIC4-CLIC5, and CLIC5- CLIC6. Finally, gene set enrichment analysis demonstrated the molecular functions and miRNA families (hsa-miR-122, hsa-miR-618, hsa-miR-425, and hsa-miR-518) relevant to the seven prognostic markers. Conclusions: These outcomes demonstrate a seven-gene molecular panel that predicts the patients’ prospective survival following pancreatic resection for PDAC.

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