Fluorescence Microscopy with Acridine Orange in Gastrointestinal Cytology

1963 ◽  
Vol 45 (6) ◽  
pp. 712-717 ◽  
Author(s):  
Lutz E. Ventzke
Keyword(s):  
Fluorescence Microscopy ◽  
Acridine Orange

scholarly journals THE ORIENTATION OF DNA WITHIN 80-ANGSTROM CHROMATIN FIBERS

1972 ◽  
Vol 52 (3) ◽  
pp. 639-647 ◽  
Author(s):  
David B. Dusenbery ◽  
Robert B. Uretz
Keyword(s):  
Electron Microscope ◽  
Fluorescence Microscopy ◽  
Salivary Glands ◽  
Acridine Orange ◽  
Average Thickness ◽  
Polarized Fluorescence ◽  
Chromatin Fibers ◽  
The Individual ◽  
Dna Packing ◽  
Chironomus Thummi

Squashing salivary glands of Chironomus thummi larvae, Amblystoma tigrinum erythrocytes, or Spirostromum frequently results in stretched chromatin having highly oriented DNA as determined by polarized fluorescence microscopy of acridine orange-stained preparations. The examination of such material from C. thummi in the electron microscope indicates that the individual chromatin fibers have an average thickness of 80 A as is usually found in embedded and sectioned material. It is thus concluded that the DNA lies nearly parallel to the axis of these chromatin fibers. Detailed calculations of the polarization expected from various models of DNA packing are contained in an appendix.

ACRIDINE ORANGE FLUOROCHROMING AND ULTRAVIOLET ABSORPTION OF STAPHYLOCOCCUS AUREUS CELLS MODIFIED BY UNBALANCED NUCLEIC ACID AND CELL WALL SYNTHESIS

1966 ◽  
Vol 12 (4) ◽  
pp. 677-682
Author(s):  
Jacques de Repentigny ◽  
Sorin Sonea ◽  
Armand Frappier
Keyword(s):  
Staphylococcus Aureus ◽  
Nucleic Acid ◽  
Fluorescence Microscopy ◽  
Nucleic Acids ◽  
Acridine Orange ◽  
Dry Weight ◽  
Cellular Level ◽  
Cellular Heterogeneity ◽  
Nucleic Acid Content ◽  
Bacterial Populations

Cultures of Staphylococcus aureus were grown in the presence of five different antimetabolites (5-fluorodeoxyuridine, aminopterin, 8-azaguanine, mitomycm-C, 5-fluorouracil) active against ceil walls and (or) nucleic acids. Fluorescence microscopy of smears stained with acridine orange revealed reddish and green cells in both treated and untreated cultures. There were less than 20% of reddish cells in untreated cultures and more than 40% in treated cultures. Treated cultures contained fewer viable organisms. All antimetabolites except mitomycin-C produced a diminution in the nucleic acids, chemically determined as percentage of dry weight of bacteria. Only 5-fluorouracil increased the RNA/DNA ratio. However, with ultraviolet microscopy at 260 mμ wavelength the absorption of reddish cells is much higher than that of the green cells, which, at the cellular level, seemed to indicate a greater nucleic acid content. With ultraviolet or with fluorescence microscopy we have obtained similar evidence of the cellular heterogeneity produced by antimetabolites in bacterial populations.

Somatic nuclear division in the sporidia of Ustilago violacea. II. Observations on living cells with phase-contrast and fluorescence microscopy

1974 ◽  
Vol 20 (5) ◽  
pp. 739-746 ◽  
Author(s):  
N. H. Poon ◽  
A. W. Day
Keyword(s):  
Fluorescence Microscopy ◽  
Acridine Orange ◽  
Phase Contrast ◽  
Nuclear Division ◽  
Spindle Pole Body ◽  
Time Lapse ◽  
Spindle Pole ◽  
Living Cells ◽  
Pole Body ◽  
Orange Fluorescence

Somatic nuclear division in living cells is described under both phase-contrast and acridine orange fluorescence microscopy. The observations confirm a previous description of the division in fixed cells stained with acetic orcein. Acridine orange at the optimum concentration of 75–250 mg/liter complete medium clearly differentiated the nucleolus, chromatinic granules, nucleoplasm, and spindle pole body, as well as indicating changes in RNA content in the cytoplasm during budding. Acridine orange fluorescence was identical in both living and fixed cells. The fluorescence of the spindle pole body indicated that it contains DNA, which may initiate RNA synthesis. Time-lapse phase-contrast observations confirmed that neither the fixation technique nor acridine orange or acetic orcein staining caused noticeable artefacts during division, and provided indisputable evidence for the sequencing of stages. Estimates from the time-lapse observations indicated that the division is quite slow (about 45 min) and that 'prophase' takes about 12 min, 'metaphase' 5 min, and 'anaphase–telophase' about 28 min.

scholarly journals Ex Vivo Confocal Fluorescence Microscopy for Rapid Evaluation of Tissues in Surgical Pathology Practice

2017 ◽  
Vol 142 (3) ◽  
pp. 396-401 ◽  
Author(s):  
Savitri Krishnamurthy ◽  
Andrea Cortes ◽  
Mirtha Lopez ◽  
Michael Wallace ◽  
Sharjeel Sabir ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Acridine Orange ◽  
Ex Vivo ◽  
Imaging Techniques ◽  
Surgical Pathology ◽  
Confocal Fluorescence Microscopy ◽  
Tissue Preparation ◽  
Tissue Sections ◽  
Confocal Fluorescence ◽  
Hematoxylin Eosin

Context.— Optical imaging techniques are currently available for imaging tissues without the need for any type of extensive tissue preparation. There are several applications for their potential use in surgical pathology practice. Objective.— To evaluate the feasibility of using a confocal fluorescence microscopy (CFM) platform for ex vivo examination of tissues obtained from surgical resections of breast, lung, kidney, and liver. Design.— Tissue fragments (0.5–1.0 cm) were immersed in 0.6 mM acridine orange for 6 seconds and imaged using a CFM platform at a 488-nm wavelength. The imaged tissues were subsequently fixed in formalin and processed routinely to generate hematoxylin-eosin–stained tissue sections. Mosaics of the grayscale CFM images were studied at different magnifications for recognition of the tissue and were compared with conventional histopathologic examination of hematoxylin-eosin tissue sections. Results.— We imaged 55 tissue fragments obtained from 16 breast (29%), 18 lung (33%), 14 kidney (25%), and 7 liver (13%) surgical excision specimens. Acridine orange labeled the nuclei, creating the contrast between nucleus and cytoplasm and thereby recapitulating the tissue architecture. We could obtain CFM images of good quality within 5 to 10 minutes that allowed recognition of the cytomorphologic details for categorization of the imaged tissue and were similar to histologic examination of hematoxylin-eosin tissue sections. Conclusions.— The ease and speed of acquisition of CFM images together with the resolution and resemblance of the CFM images to hematoxylin-eosin sections suggest that the CFM platform has excellent potential for use in surgical pathology practice.

scholarly journals Reliability of acridine orange fluorescence microscopy in oral cytodiagnosis

2011 ◽  
Vol 22 (5) ◽  
pp. 649 ◽  
Author(s):  
Nilima Prakash ◽  
GL Pradeep ◽  
P Sharada ◽  
N Soundarya
Keyword(s):  
Fluorescence Microscopy ◽  
Acridine Orange ◽  
Orange Fluorescence ◽  
Acridine Orange Fluorescence
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