Non-adrenergic non-chlinergic (NANC) inhibition in longitudinal smooth muscle of rat jejunum: Minor role of nitric oxide (NO)

Intact and α-toxin-permeabilized longitudinal smooth muscle were mounted for measurement of force and myosin light chain phosphorylation. Galanin contracted intact jejunum with a half-maximum effective concentration of 9.2 ± 0.1 nM. Neither atropine, hexamethonium, guanethidine, nor tetrodotoxin affected the contraction. The contraction was also unaffected by depletion of intracellular Ca2+ or by addition of thapsigargin; removal of extracellular Ca2+ or addition of nifedipine abolished the contraction. Galanin increased myosin light chain phosphorylation levels concomitantly with force. During continued tissue stimulation, force fell to suprabasal values, whereas myosin light chain phosphorylation levels remained elevated. Galanin increased Ca2+ sensitivity of contraction in α-toxin-permeabilized tissues, and this was reversed by either guanosine 5′- O-(2-thiodiphosphate) or pertussis toxin. These results suggest that galanin-induced contraction of longitudinal jejunal smooth muscle is dependent on a pertussis toxin-sensitive G protein that is apparently not coupled to the release of intracellular Ca2+but to the influx of extracellular Ca2+ and involves an initial myofilament Ca2+ sensitization followed by Ca2+ desensitization.

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The Role of M2 Muscarinic Receptor Subtypes Mediating Contraction of the Circular and Longitudinal Smooth Muscle of the Pig Proximal Urethra

The Journal of Urology ◽

10.1016/s0022-5347(05)64913-0 ◽

2002 ◽

Vol 168 (1) ◽

pp. 308-314 ◽

Cited By ~ 19

Author(s):

Tomonori Yamanishi ◽

Christopher R. Chapple ◽

Kosaku Yasuda ◽

Ken-Ichiro Yoshida ◽

Russell Chess-Williams

Keyword(s):

Smooth Muscle ◽

Muscarinic Receptor ◽

Receptor Subtypes ◽

Muscarinic Receptor Subtypes ◽

Longitudinal Smooth Muscle ◽

M2 Muscarinic Receptor ◽

Proximal Urethra

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Role of nitric oxide in human esophageal circular smooth muscle in vitro

Journal of Thoracic and Cardiovascular Surgery ◽

10.1016/s0022-5223(05)80021-2 ◽

1995 ◽

Vol 110 (1) ◽

pp. 157-164 ◽

Cited By ~ 14

Author(s):

William G. Richards ◽

Jonathan S. Stamler ◽

Lester Kobzik ◽

David J. Sugarbaker

Keyword(s):

Nitric Oxide ◽

Smooth Muscle ◽

Circular Smooth Muscle

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A structured review on the female urethral anatomy and innervation with an emphasis on the role of the urethral longitudinal smooth muscle

International Urogynecology Journal ◽

10.1007/s00192-019-04104-7 ◽

2019 ◽

Vol 31 (1) ◽

pp. 63-71 ◽

Cited By ~ 3

Author(s):

Michelle Alexandra Mistry ◽

Niels Klarskov ◽

John O. DeLancey ◽

Gunnar Lose

Keyword(s):

Smooth Muscle ◽

Longitudinal Smooth Muscle

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Nitric oxide production in murine spleen cells: role of interferons and prostaglandin E2in the generation of cytotoxic activity

Mediators of Inflammation ◽

10.1155/s0962935196000117 ◽

1996 ◽

Vol 5 (1) ◽

pp. 62-68 ◽

Cited By ~ 1

Author(s):

Dominique Vaillier ◽

Richard Daculsi ◽

Norbert Gualdel

Keyword(s):

Nitric Oxide ◽

Cytotoxic Activity ◽

Minor Role ◽

No Production ◽

Spleen Cells ◽

Kinase C ◽

Murine Spleen ◽

Ifn Γ ◽

A Minor

The production of nitric oxide (NO) was measured in cultures of spleen cells stimulated by lipopolysaccharide (LPS), IL-2 or LPS + IL-2. We observed that NO synthesis is increased by IFN-γ but inhibited by IFN-α/β. This is not the case when IL-2 is present in the cultures, since interferons play a minor role in the regulation of the NO production. When IL-2 and LPS were associated in the cultures, the IFN-α/β role seems more important than that of IFN-γ. PGE2inhibits NO production in LPS supplemented cultures but has a slight effect in the presence of IL-2 and no effect with IL-2 + LPS. 3-isoButyl-1-methylxanthine (IBMX), an inhibitor of phosphodiesterases, induces a decrease of IFN production. In the presence of H-7, an inhibitor of protein kinase C (PKC), NO production is reduced when the cultures are supplemented by LPS or IL-2 but not when IL-2 and LPS are both added. H-7 also reduced IFN production. In the presence of NG-monomethyl-L-arginine (N-MMA), an inhibitor of NO synthesis, IFN production was increased, with no change in the cytotoxic activity. Hence, interferons regulate NO production by mouse spleen cells and, in return, NO modulates the generation of IFN.

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