Detection of genitourinary tract Chlamydia trachomatis infection in pregnant women by ligase chain reaction assay

1997 ◽  
Vol 58 (3) ◽  
pp. 351-351
Keyword(s):  
Chlamydia Trachomatis ◽  
Pregnant Women ◽  
Genitourinary Tract ◽  
Chain Reaction ◽  
Ligase Chain Reaction ◽  
Chlamydia Trachomatis Infection

scholarly journals Comparison of the ligase chain reaction with cell culture for the diagnosis of Chlamydia trachomatis infection in women.

1996 ◽  
Vol 49 (2) ◽  
pp. 116-119 ◽  
Author(s):  
G L Ridgway ◽  
G Mumtaz ◽  
A J Robinson ◽  
M Franchini ◽  
C Carder ◽  
...  
Keyword(s):  
Cell Culture ◽  
Chlamydia Trachomatis ◽  
Chain Reaction ◽  
Ligase Chain Reaction ◽  
Chlamydia Trachomatis Infection

scholarly journals Comparison of Performances of Two Commercially Available Tests, a PCR Assay and a Ligase Chain Reaction Test, in Detection of Urogenital Chlamydia trachomatis Infection

1998 ◽  
Vol 36 (6) ◽  
pp. 1489-1493 ◽  
Author(s):  
Mirja Puolakkainen ◽  
Eija Hiltunen-Back ◽  
Timo Reunala ◽  
Satu Suhonen ◽  
Pekka Lähteenmäki ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Clinical Laboratory ◽  
Transmitted Disease ◽  
Chain Reaction ◽  
Ligase Chain Reaction ◽  
Chlamydia Trachomatis Infection ◽  
Sexually Transmitted ◽  
Transport Medium ◽  
Sexually Transmitted Disease Clinic ◽  
Chain Reaction Test

The diagnostic performance of a PCR test (Roche Cobas Amplicor CT/NG Test) and that of a ligase chain reaction (LCR) test (Abbott LCxChlamydia trachomatis assay) were compared by using endocervical and urethral swab specimen culture as a reference test. First-void urine (FVU) and endocervical and urethral swab specimens were collected from 1,015 unselected patients attending a sexually transmitted disease clinic and a clinic for adolescents in Helsinki, Finland. Chlamydia trachomatis was cultured from samples from the endocervix or urethra. PCR was performed with fresh and frozen urine and the culture transport medium. LCR was performed with fresh and frozen urine and LCx swab transport medium. Diagnostic consistency and diagnostic accuracy were statistically tested. The test results were identical for 984 patients (97%). Discrepant results were observed for 31 patients. Overall, LCR and PCR showed excellent kappa coefficients of consistency for both swab and FVU specimens (0.93 and 0.95, respectively). Sixty-one patients (6%) were culture positive. Testing of FVU by LCR or PCR increased the overall positivity rates to 7.0 and 7.7%, respectively. While PCR of FVU detected the greatest number of C. trachomatis infections (sensitivity, 96.1%), for some PCR-positive FVU specimens the results could not be confirmed (specificity, 99.6%). PCR and LCR were more sensitive than culture (sensitivities, 92 and 93% versus 79% for culture) in the diagnosis of genital C. trachomatis infection. In conclusion, both tests can be recommended for use in the clinical laboratory and for the screening of asymptomatic C. trachomatis infections.

scholarly journals Use of the ligase chain reaction on urine of men and their female sexual partners for detection of genital Chlamydia trachomatis infection

1996 ◽  
Vol 2 (2) ◽  
pp. 123-126 ◽  
Author(s):  
Fabio Rurnpianesi ◽  
Manuela Donati ◽  
Michelangelo La Placa ◽  
Massinzo Negosanti ◽  
Antonietta D'Antuono ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Sexual Partners ◽  
Chain Reaction ◽  
Ligase Chain Reaction ◽  
Chlamydia Trachomatis Infection

scholarly journals Detection of Chlamydia trachomatis infection in urine samples from men and women by ligase chain reaction.

1995 ◽  
Vol 33 (8) ◽  
pp. 2042-2047 ◽  
Author(s):  
G J van Doornum ◽  
M Buimer ◽  
M Prins ◽  
C J Henquet ◽  
R A Coutinho ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Urine Samples ◽  
Chain Reaction ◽  
Men And Women ◽  
Ligase Chain Reaction ◽  
Chlamydia Trachomatis Infection

scholarly journals Urine Specimens from Pregnant and Nonpregnant Women Inhibitory to Amplification of Chlamydia trachomatis Nucleic Acid by PCR, Ligase Chain Reaction, and Transcription-Mediated Amplification: Identification of Urinary Substances Associated with Inhibition and Removal of Inhibitory Activity

1998 ◽  
Vol 36 (11) ◽  
pp. 3122-3126 ◽  
Author(s):  
J. Mahony ◽  
S. Chong ◽  
D. Jang ◽  
K. Luinstra ◽  
M. Faught ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Chlamydia Trachomatis ◽  
Pregnant Women ◽  
False Negative ◽  
Nucleic Acid Amplification ◽  
Urine Specimen ◽  
Chain Reaction ◽  
Ligase Chain Reaction ◽  
Negative Results ◽  
Urine Specimens

The presence of endogenous amplification inhibitors in urine may produce false-negative results for the detection of Chlamydia trachomatis nucleic acids by tests such as PCR, ligase chain reaction (LCR), and transcription-mediated amplification (TMA). Consecutive urine specimens from 101 pregnant women and 287 nonpregnant women submitted for urinalysis were processed for C. trachomatis detection. Aliquots were spiked with the equivalent of one C. trachomatis elementary body and were tested by three commercial assays: AMPLICOR CT/NG, Chlamydia LCX, and Chlamydia TMA. The prevalence of inhibitors resulting in complete inhibition of amplification was 4.9% for PCR, 2.6% for LCR, and 7.5% for TMA. In addition, all three assays were partially inhibited by additional urine specimens. Only PCR was more often inhibited by urine from pregnant women than by urine from nonpregnant women (9.9 versus 3.1%;P = 0.011). A complete urinalysis including dipstick and a microscopic examination was performed. Logistic regression analysis revealed that the following substances were associated with amplification inhibition: beta-human chorionic gonadotropin (odds ratio [OR], 3.3) and crystals (OR, 3.3) for PCR, nitrites for LCR (OR, 14.4), and hemoglobin (OR, 3.3), nitrites (OR, 3.3), and crystals (OR, 3.3) for TMA. Aliquots of each inhibitory urine specimen were stored at 4 and −70°C overnight or were extracted with phenol-chloroform and then retested at dilutions of 1:1, 1:4, and 1:10. Most inhibition was removed by storage overnight at 4 or −70°C and a dilution of 1:10 (84% for PCR, 100% for LCR, and 92% for TMA). Five urine specimens (three for PCR and two for TMA) required phenol-chloroform extraction to remove inhibitors. The results indicate that the prevalence of nucleic acid amplification inhibitors in female urine is different for each technology, that this prevalence may be predicted by the presence of urinary factors, and that storage and dilution remove most of the inhibitors.

scholarly journals Use of Ligase Chain Reaction with Urine versus Cervical Culture for Detection of Chlamydia trachomatis in an Asymptomatic Military Population of Pregnant and Nonpregnant Females Attending Papanicolaou Smear Clinics

1998 ◽  
Vol 36 (5) ◽  
pp. 1300-1304 ◽  
Author(s):  
Charlotte A. Gaydos ◽  
M. Rene Howell ◽  
Thomas C. Quinn ◽  
Joel C. Gaydos ◽  
Kelly T. McKee
Keyword(s):  
Chlamydia Trachomatis ◽  
Pregnant Women ◽  
Fluorescent Antibody ◽  
Chain Reaction ◽  
Military Population ◽  
Predictive Values ◽  
Papanicolaou Smear ◽  
Ligase Chain Reaction ◽  
Culture Positive ◽  
Urine Specimens

Ligase chain reaction (LCR) (Abbott Laboratories, Abbott Park, Ill.) with first-catch urine specimens was used to detectChlamydia trachomatis infections in 465 asymptomatic military women attending clinics for routine Papanicolaou smear tests. Results were compared to results of cervical culture to determine the sensitivity of the urine LCR and the possible presence of inhibitors of amplification in pregnant and nonpregnant women. Discrepant results for LCR and culture were resolved by direct fluorescent antibody staining of culture sediments, two different PCR assays, and LCR for the outer membrane protein 1 gene. The prevalence of Chlamydia in specimens by urine LCR was 7.3% compared to 5% by culture. For 434 women with matching specimens, there were 11 more specimens positive by LCR than were positive by culture, of which all but one were determined to be true positives. There were four culture-positive, LCR-negative specimens, all from nonpregnant women. The sensitivity, specificity, and positive and negative predictive values of urine LCR after discrepant results were resolved were 88.6, 99.7, 96.9, and 99.0%, respectively. The sensitivity of culture was 71.4%. From the 148 pregnant women (prevalence by LCR, 6.8%), there were no patients who were cervical culture positive and urine LCR negative to indicate the presence in pregnant women of inhibitors of LCR. Additionally, a subset of 55 of the LCR-negative frozen urine specimens from pregnant women that had been previously processed in LCR buffer were inoculated with 5 cell culture inclusion forming units of C. trachomatis each and retested by LCR; all tested positive, indicating the absence of inhibitors of LCR in urine from these pregnant women. The use of LCR testing of urine specimens from asymptomatic women, whether pregnant or not, offers a sensitive and easy method to detect C. trachomatis infection in women.

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