Mapping of linear B-cell epitopes on the 14-kDa fatty-acid binding protein of Chinese Schistosoma japonicum

1998 ◽  
Vol 28 (2) ◽  
pp. 303-308 ◽  
Author(s):  
G Waine
Keyword(s):  
Fatty Acid ◽  
B Cell ◽  
Schistosoma Japonicum ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Fatty Acid Binding ◽  
B Cell Epitopes ◽  
Acid Binding Protein ◽  
Linear B

Immunolocalization of the fatty acid-binding protein Sj-FABPc within adult Schistosoma japonicum

Parasitology ◽  
1997 ◽  
Vol 115 (1) ◽  
pp. 33-39 ◽  
Author(s):  
G. N. GOBERT ◽  
D. J. STENZEL ◽  
M. K. JONES ◽  
D. P. McMANUS
Keyword(s):  
Fatty Acid ◽  
Schistosoma Japonicum ◽  
Lipid Droplets ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Transmission Electron ◽  
Male Parasite ◽  
Gold Marker

This paper describes the first localization study of the 14·7 kDa fatty acid-binding protein in Schistosoma japonicum (Sj-FABPc) using transmission electron microscopy. A polyclonal antibody raised against recombinant Sj-FABPc was used in combination with a colloidal gold marker to determine the distribution of the protein within adult parasites. Sj-FABPc was localized within lipid droplets below the subtegumental region of the male parasite. Additionally, Sj-FABPc was present in the vitelline droplets of the vitelline glands of female parasites. There were no detectable levels of Sj-FABPc on the surface or within the tegument of male or female parasites. Possible functions of Sj-FABPc within S. japonicum and the relevance of these immunolocalization findings in light of the recent reports that the homologue Sm-FABPc is an important anti-S. mansoni vaccine target molecule are also discussed.

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