scholarly journals Insulin receptors and insulin modulation of norepinephrine uptake in neuronal cultures from rat brain.

1985 ◽  
Vol 260 (29) ◽  
pp. 15880-15884 ◽  
Author(s):  
F T Boyd ◽  
D W Clarke ◽  
T F Muther ◽  
M K Raizada
Keyword(s):  
Rat Brain ◽  
Insulin Receptors ◽  
Neuronal Cultures ◽  
Norepinephrine Uptake

Insulin inhibits specific norepinephrine uptake in neuronal cultures from rat brain

1986 ◽  
Vol 398 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Frederick T. Boyd ◽  
Derrel W. Clarke ◽  
Mohan K. Raizada
Keyword(s):  
Rat Brain ◽  
Neuronal Cultures ◽  
Norepinephrine Uptake

scholarly journals Oxidative Stress Induces Dephosphorylation of τ in Rat Brain Primary Neuronal Cultures

2002 ◽  
Vol 68 (4) ◽  
pp. 1590-1597 ◽  
Author(s):  
Daniel R. Davis ◽  
Brian H. Anderton ◽  
Jean-Pierre Brion ◽  
C. Hugh Reynolds ◽  
Diane P. Hanger
Keyword(s):  
Oxidative Stress ◽  
Rat Brain ◽  
Neuronal Cultures ◽  
Primary Neuronal Cultures

ANP receptors in neurons and astrocytes from spontaneously hypertensive rat brain

1993 ◽  
Vol 265 (1) ◽  
pp. C106-C112 ◽  
Author(s):  
W. Tang ◽  
W. R. Paulding ◽  
C. Sumners
Keyword(s):  
Rat Brain ◽  
Natriuretic Peptide ◽  
Spontaneously Hypertensive Rat ◽  
Binding Capacity ◽  
Specific Binding ◽  
Large Population ◽  
Neuronal Cultures ◽  
Spontaneously Hypertensive ◽  
Glial Cultures ◽  
Anp Receptors

In this study we compared the levels and responsiveness of atrial natriuretic peptide (ANP) receptors in neuronal and astrocyte glial cultures from spontaneously hypertensive (SH) and normotensive (Wistar-Kyoto: WKY) rat brain. Both neuronal and astrocyte glial cultures from the hypothalamus and brain stem of 1-day-old SH and WKY rats display specific high-affinity binding sites for 125I-labeled ANP. The presence of a large population of ANP-C receptors in each type of culture is indicated by the strong competition of 125I-ANP binding by the ring-deleted analogue of ANP [C-ANF-(4-23)]. In neuronal cultures from both strains, C-type natriuretic peptide (CNP-22) was the most effective natriuretic peptide in stimulating guanosine 3',5'-cyclic monophosphate (cGMP) levels, suggesting the presence of ANP-B receptors in these cells. By contrast, ANP was the most effective stimulator of cGMP levels in SH and WKY rat astrocyte glial cultures, suggesting the presence of ANP-A receptors. Here, we have determined that there is a decrease in the maximum binding capacity for 125I-ANP-specific binding in both SH rat neuronal and astrocyte glial cultures compared with their respective control cells. The stimulatory effects of CNP-22 on cGMP levels in SH rat neurons and of ANP on cGMP levels in SH rat astrocytes were significantly reduced compared with their respective WKY rat cultures. Our data suggest that the lower number of ANP receptors in SH rat neuronal and astrocyte glial cultures includes a reduction in the guanylate cyclase-coupled ANP receptors.

Decreased alpha 1-adrenergic receptor-mediated inositide hydrolysis in neurons from hypertensive rat brain

1986 ◽  
Vol 251 (2) ◽  
pp. C230-C237 ◽  
Author(s):  
J. B. Feldstein ◽  
R. A. Gonzales ◽  
S. P. Baker ◽  
C. Sumners ◽  
F. T. Crews ◽  
...  
Keyword(s):  
Rat Brain ◽  
Adrenergic Receptors ◽  
Inositol Phosphates ◽  
Inositol Phosphate ◽  
Specific Binding ◽  
Membrane Receptors ◽  
Neuronal Cultures ◽  
Brain Neurons ◽  
Neuronal Membranes ◽  
Rat Brain Neurons

The expression of alpha 1-adrenergic receptors and norepinephrine (NE)-stimulated hydrolysis of inositol phospholipid has been studied in neuronal cultures from the brains of normotensive (Wistar-Kyoto, WKY) and spontaneously hypertensive (SH) rats. Binding of 125I-2-[beta-(4-hydroxyphenyl)-ethyl-aminomethyl] tetralone (HEAT) to neuronal membranes was 68-85% specific and was rapid. Competition-inhibition experiments with various agonists and antagonists suggested that 125I-HEAT bound selectively to alpha 1-adrenergic receptors. Specific binding of 125I-HEAT to neuronal membranes from SH rat brain cultures was 30-45% higher compared with binding in WKY normotensive controls. This increase was attributed to an increase in the number of alpha 1-adrenergic receptors on SH rat brain neurons. Incubation of neuronal cultures of rat brain from both strains with NE resulted in a concentration-dependent stimulation of release of inositol phosphates, although neurons from SH rat brains were 40% less responsive compared with WKY controls. The decrease in responsiveness of SH rat brain neurons to NE, even though the alpha 1-adrenergic receptors are increased, does not appear to be due to a general defect in membrane receptors and postreceptor signal transduction mechanisms. This is because neither the number of muscarinic-cholinergic receptors nor the carbachol-stimulated release of inositol phosphates is different in neuronal cultures from the brains of SH rats compared with neuronal cultures from the brains of WKY rats. These observations suggest that the increased expression of alpha 1-adrenergic receptors does not parallel the receptor-mediated inositol phosphate hydrolysis in neuronal cultures from SH rat brain.

scholarly journals Oxytocin receptors on cultured astroglial cells. Kinetic and pharmacological characterization of oxytocin-binding sites on intact hypothalamic and hippocampic cells from foetal rat brain

1992 ◽  
Vol 284 (2) ◽  
pp. 491-497 ◽  
Author(s):  
D Di Scala-Guenot ◽  
M T Strosser
Keyword(s):  
Rat Brain ◽  
Cell Cultures ◽  
Computer Analysis ◽  
Binding Sites ◽  
Hill Coefficient ◽  
Neuronal Cultures ◽  
Astroglial Cells ◽  
Intact Cells ◽  
Homogeneous Population ◽  
Hypothalamic Cells

The ability of astroglial cells to exhibit oxytocin (OT)-binding sites has been investigated in embryonic hypothalamic and hippocampic astroglial cell cultures. The differential characteristics of binding of OT and [Arg8]vasopressin (AVP) agonists and antagonists to the OT-binding sites using the highly selective iodinated OT antagonist d(CH2)5-[Tyr(Me)2,Thr4,Tyr-NH2(9)]OVT ([125I]OTA) have been evaluated using intact cells maintained for 12 days in culture. The specific binding displayed features of reversibility. Computer analysis of the saturation studies using the LIGAND program indicated that, at 4 degrees C, the antagonist binds to a homogeneous population of sites with a Kd value of 0.02 nM and a low binding-site density of around 2 fmol/dish for hypothalamic cells and 6 fmol/dish for hippocampic cells. For hypothalamic cells, competition curves using unlabelled OT, AVP or V2 AVP agonist were characterized by a pseudo-Hill coefficient below unity (0.7), indicating possible heterogeneity among the binding sites. On the other hand, the dose-inhibition curves resulting from competition studies with hippocampic cells had a pseudo-Hill coefficient close to unity, except for OT. Computer analysis (LIGAND) indicated that the OT dose-inhibition curve was significantly better fitted to a two-site model, and this can be explained by two apparent forms of the receptor having high and low affinities for the displacing drug. The relative potencies of the peptides tested for binding to the high-affinity site were: AVP greater than OT greater than V1 AVP antagonist ([d(CH2)5-Tyr(Me)2]AVP) = V2 AVP agonist greater than AVP-Sar ([d(CH2)5-Sar7,Arg8]VP) in hypothalamic cultures, and OT = AVP greater than V1 AVP antagonist greater than V2 AVP agonist in hippocampic cultures. In addition, autoradiography allowed visualization of OT-binding sites, which are located on both soma and processes of astrocyte-like type of cells. In conclusion, these data provide evidence that glial cell cultures contain specific OT-binding sites which display pharmacological characteristics different from those already reported in neuronal cultures and in the adult rat brain.

Sign in / Sign up

Export Citation Format

Share Document