scholarly journals The relationship of intracytoplasmic membrane assembly to the cell division cycle in Rhodopseudomonas sphaeroides.

1979 ◽  
Vol 254 (6) ◽  
pp. 1980-1986 ◽  
Author(s):  
R.T. Fraley ◽  
D.R. Lueking ◽  
S. Kaplan
Keyword(s):  
Cell Division ◽  
Cell Division Cycle ◽  
Rhodopseudomonas Sphaeroides ◽  
Intracytoplasmic Membrane ◽  
Membrane Assembly ◽  
Relationship Of ◽  
The Relationship

Test for temporal or spatial restrictions in gene product function during the cell division cycle

1983 ◽  
Vol 3 (7) ◽  
pp. 1255-1265
Author(s):  
S K Dutcher ◽  
L H Hartwell
Keyword(s):  
Cell Division ◽  
Gene Product ◽  
Gene Mutations ◽  
Cell Division Cycle ◽  
Temperature Sensitive ◽  
Restrictive Temperature ◽  
Product Function ◽  
Complete Cell ◽  
Relationship Of ◽  
Gene Product Function

The ability of a functional gene to complement a nonfunctional gene may depend upon the intracellular relationship of the two genes. If so, the function of the gene product in question must be limited in time or in space. CDC (cell division cycle) gene products of Saccharomyces cerevisiae control discrete steps in cell division; therefore, they constitute reasonable candidates for genes that function with temporal or spatial restrictions. In an attempt to reveal such restrictions, we compared the ability of a CDC gene to complement a temperature-sensitive cdc gene in diploids where the genes are located within the same nucleus to complementation in heterokaryons where the genes are located in different nuclei. In CDC X cdc matings, complementation was monitored in rare heterokaryons by assaying the production of cdc haploid progeny (cytoductants) at the restrictive temperature. The production of cdc cytoductants indicates that the cdc nucleus was able to complete cell division at the restrictive temperature and implies that the CDC gene product was provided by the other nucleus or by cytoplasm in the heterokaryon. Cytoductants from cdc28 or cdc37 crosses were not efficiently produced, suggesting that these two genes are restricted spatially or temporally in their function. We found that of the cdc mutants tested 33 were complemented; cdc cytoductants were recovered at least as frequently as CDC cytoductants. A particularly interesting example was provided by the CDC4 gene. Mutations in CDC4 were found previously to produce a defect in both cell division and karyogamy. Surprisingly, the cell division defect of cdc4 nuclei is complemented by CDC4 nuclei in a heterokaryon, whereas the karyogamy defect is not.

scholarly journals Cell Division in the Formation of the Stomatal Complex of the Young Leaves of Wheat

1966 ◽  
Vol 1 (1) ◽  
pp. 121-128
Author(s):  
J. D. PICKETT-HEAPS ◽  
D. H. NORTHCOTE
Keyword(s):  
Cell Division ◽  
Mother Cell ◽  
Cell Plate ◽  
Stomatal Complex ◽  
Guard Mother Cell ◽  
Young Leaves ◽  
Subsidiary Cells ◽  
Relationship Of ◽  
The Relationship ◽  
Characteristic Manner

During the formation of stomata in the young leaves of wheat the cells divide in a characteristic manner; two of the cell divisions are asymmetrical and produce cells of unequal sizes. A study of the fine structure of the cells during mitosis has shown that a band of microtubules appears at each preprophase stage. This band, although it is not present in the subsequent stages of mitosis, indicates the location on the wall of the mother cell where the cell plate will join it at the final division of the cytoplasm at telophase. Thus the future plane of cell division is indicated by these microtubules at preprophase. Microtubules are also found at the growing edge of the cell plate and appear to function in directing the vesicles which are brought up to extend the plate. The cell plate which is formed to cut off the subsidiary cells on either side of the guard mother cell is curved, and the microtubules present in conjunction with this plate during its formation could function to align and hold it on the required position. The relationship of the guard mother cell to the divisions of the adjacent epidermal cells which form the subsidiary cells is discussed, and related to general problems of growth and differentiation.

scholarly journals THE RELATIONSHIP OF CILIA WITH CELL DIVISION AND DIFFERENTIATION

1971 ◽  
Vol 49 (1) ◽  
pp. 226-229 ◽  
Author(s):  
Virginia G. Fonte ◽  
Robert L. Searls ◽  
S. Robert Hilfer
Keyword(s):  
Cell Division ◽  
Relationship Of ◽  
The Relationship

The role of yan in mediating the choice between cell division and differentiation

Development ◽  
1995 ◽  
Vol 121 (12) ◽  
pp. 3947-3958 ◽  
Author(s):  
R. Rogge ◽  
P.J. Green ◽  
J. Urano ◽  
S. Horn-Saban ◽  
M. Mlodzik ◽  
...  
Keyword(s):  
Cell Division ◽  
Growth Factor Receptor ◽  
Developmental Context ◽  
Eye Disc ◽  
A Cell ◽  
Epidermal Growth ◽  
Notch Pathways ◽  
Relationship Of ◽  
The Relationship

An allele of the yan locus was isolated as an enhancer of the Ellipse mutation of the Drosophila epidermal growth factor receptor (Egfr) gene. This yan allele is an embryonic lethal and also fails to complement the lethality of anterior open (aop) mutations. Phenotypic and complementation analysis revealed that aop is allelic to yan and genetically the lethal alleles act as null mutations for the yan gene. Analysis of the lethal alleles in the embryo and in mitotic clones showed that loss of yan function causes cells to overproliferate in the dorsal neuroectoderm of the embryo and in the developing eye disc. Our studies suggest that the role of yan is defined by the developmental context of the cells in which it functions. An important role of this gene is in allowing a cell to choose between cell division and differentiation. The relationship of the Egfr and Notch pathways to this developmental role of yan is discussed.

scholarly journals Test for temporal or spatial restrictions in gene product function during the cell division cycle.

1983 ◽  
Vol 3 (7) ◽  
pp. 1255-1265 ◽  
Author(s):  
S K Dutcher ◽  
L H Hartwell
Keyword(s):  
Cell Division ◽  
Gene Product ◽  
Gene Mutations ◽  
Cell Division Cycle ◽  
Temperature Sensitive ◽  
Restrictive Temperature ◽  
Product Function ◽  
Complete Cell ◽  
Relationship Of ◽  
Gene Product Function

The ability of a functional gene to complement a nonfunctional gene may depend upon the intracellular relationship of the two genes. If so, the function of the gene product in question must be limited in time or in space. CDC (cell division cycle) gene products of Saccharomyces cerevisiae control discrete steps in cell division; therefore, they constitute reasonable candidates for genes that function with temporal or spatial restrictions. In an attempt to reveal such restrictions, we compared the ability of a CDC gene to complement a temperature-sensitive cdc gene in diploids where the genes are located within the same nucleus to complementation in heterokaryons where the genes are located in different nuclei. In CDC X cdc matings, complementation was monitored in rare heterokaryons by assaying the production of cdc haploid progeny (cytoductants) at the restrictive temperature. The production of cdc cytoductants indicates that the cdc nucleus was able to complete cell division at the restrictive temperature and implies that the CDC gene product was provided by the other nucleus or by cytoplasm in the heterokaryon. Cytoductants from cdc28 or cdc37 crosses were not efficiently produced, suggesting that these two genes are restricted spatially or temporally in their function. We found that of the cdc mutants tested 33 were complemented; cdc cytoductants were recovered at least as frequently as CDC cytoductants. A particularly interesting example was provided by the CDC4 gene. Mutations in CDC4 were found previously to produce a defect in both cell division and karyogamy. Surprisingly, the cell division defect of cdc4 nuclei is complemented by CDC4 nuclei in a heterokaryon, whereas the karyogamy defect is not.

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