Cross-linking of stroma-free hemoglobin monitored by high-performance liquid chromatography

1982 ◽  
Vol 246 (1) ◽  
pp. 65-71 ◽  
Author(s):  
Piergiorgio Pietta ◽  
Alma Calatroni ◽  
Gabriele Palazzini ◽  
Angelo Agostoni
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Cross Linking ◽  
Free Hemoglobin

scholarly journals Preparation of rosin-based polymer microspheres as a stationary phase in high-performance liquid chromatography to separate polycyclic aromatic hydrocarbons and alkaloids

e-Polymers ◽  
2019 ◽  
Vol 19 (1) ◽  
pp. 290-296
Author(s):  
Zhen Wang ◽  
Fu-Hou Lei ◽  
Wen Li ◽  
Peng-Fei Li ◽  
Ting Wang ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Polycyclic Aromatic Hydrocarbons ◽  
Liquid Chromatography ◽  
Stationary Phase ◽  
Aromatic Hydrocarbons ◽  
High Performance ◽  
Cross Linking ◽  
Polymer Microspheres ◽  
Polycyclic Aromatic ◽  
Microsuspension Polymerization

AbstractAcrylpimaric acid ethylene glycol acrylate was used as the cross-linking agent to prepare rosin-based polymer microspheres through membrane emulsification–microsuspension polymerization. The prepared microspheres were used as the stationary phase in high-performance liquid chromatography for the separation of polycyclic aromatic hydrocarbons (PAHs) and two analogues of alkaloids. Five PAHs (benzene, naphthalene, fluorene, pyrene, and benzo(e)pyrene) were well separated in the rosin-based polymer column with the resolution (Rs) of 6.088 ± 0.006, 5.759 ± 0.017, 7.019 ± 0.020, and 8.584 ± 0.063. A linear relationship was observed for the carbon numbers of PAHs and lnK as obtained with the equation of ln k = 0.22nc – 2.45, R2 = 0.996 . The rosin-based polymer columns were also employed to separate theophylline–caffeine and camptothecin–10-hydroxy-camptothecin, and the good results were obtained with the Rs of 4.617 ± 0.005 and 2.245 ± 0.049, respectively.

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