scholarly journals Preparation of rosin-based polymer microspheres as a stationary phase in high-performance liquid chromatography to separate polycyclic aromatic hydrocarbons and alkaloids

e-Polymers ◽  
2019 ◽  
Vol 19 (1) ◽  
pp. 290-296
Author(s):  
Zhen Wang ◽  
Fu-Hou Lei ◽  
Wen Li ◽  
Peng-Fei Li ◽  
Ting Wang ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Polycyclic Aromatic Hydrocarbons ◽  
Liquid Chromatography ◽  
Stationary Phase ◽  
Aromatic Hydrocarbons ◽  
High Performance ◽  
Cross Linking ◽  
Polymer Microspheres ◽  
Polycyclic Aromatic ◽  
Microsuspension Polymerization

AbstractAcrylpimaric acid ethylene glycol acrylate was used as the cross-linking agent to prepare rosin-based polymer microspheres through membrane emulsification–microsuspension polymerization. The prepared microspheres were used as the stationary phase in high-performance liquid chromatography for the separation of polycyclic aromatic hydrocarbons (PAHs) and two analogues of alkaloids. Five PAHs (benzene, naphthalene, fluorene, pyrene, and benzo(e)pyrene) were well separated in the rosin-based polymer column with the resolution (Rs) of 6.088 ± 0.006, 5.759 ± 0.017, 7.019 ± 0.020, and 8.584 ± 0.063. A linear relationship was observed for the carbon numbers of PAHs and lnK as obtained with the equation of ln k = 0.22nc – 2.45, R2 = 0.996 . The rosin-based polymer columns were also employed to separate theophylline–caffeine and camptothecin–10-hydroxy-camptothecin, and the good results were obtained with the Rs of 4.617 ± 0.005 and 2.245 ± 0.049, respectively.

High performance liquid chromatography–tandem mass spectrometry for rapid and sensitive analysis of six polycyclic aromatic hydrocarbons in wastewater

2011 ◽  
Vol 64 (2) ◽  
pp. 477-484 ◽  
Author(s):  
Manli Wu ◽  
Huining Xu ◽  
Ya Yu ◽  
Lili Wang
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Polycyclic Aromatic Hydrocarbons ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Aromatic Hydrocarbons ◽  
Mobile Phase ◽  
High Performance ◽  
Tandem Mass ◽  
Polycyclic Aromatic

A sensitive and fast method was developed to quantitatively analyse the six polycyclic aromatic hydrocarbons (fluoranthene (FLT), benzo[b]fluoranthene (BbF), benzo[k]fluoranthene (BkF), benzo[a]pyrene (Bap), benzo[ghi]perylene (BghiP) and indeno[1,2,3-cd]pyrene (INPY)) by high performance liquid chromatography (UPLC) coupling with tandem mass spectrometry (MS/MS). Chromatographic separation was performed on a Waters Acquity UPLCTM BEHC18 column (1.7 μm, 2.1 mm × 50 mm). A 0.2 μm precolumn filter was used to protect the analytical column. Mobile phase A was acetonitrile containing 0.5% toluene. Mobile phase B was water. Linearity of detection was in the range of 1–100 μg L−1; LOD of 5 PAHs were lower than 0.1 μg L−1; LOQ were 0.2 μg L−1 except for benzo[k]fluoranthene. The LOD and the LOQ of benzo[k]fluoranthene were respectively 0.1 μg L−1 and 0.8 μg L−1. Wastewater samples collected from two wastewater treatment plants were determined using this method respectively. Recovery of all compounds varied from 67.8 ± 10.6% to 113.2 ± 7.2%. In comparison with the existing methods, this rapid method saves time and solvent and improves instrument sample throughput by 2–5 fold.

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