Improved two-dimensional solvent system for thin-layer chromatographic analysis of polar lipids on silica gel 60 precoated plates

1978 ◽  
Vol 152 (2) ◽  
pp. 569-574 ◽  
Author(s):  
Jacques Portoukalian ◽  
Roger Meister ◽  
Georges Zwingelstein
Keyword(s):  
Thin Layer ◽  
Silica Gel ◽  
Chromatographic Analysis ◽  
Solvent System ◽  
Polar Lipids ◽  
Two Dimensional ◽  
Thin Layer Chromatographic Analysis

scholarly journals Thin Layer Chromatographic Analysis of Food Colorants from Three Morphotypes of Annatto (Bixa orellana L.)

2013 ◽  
Vol 2 (1) ◽  
pp. 7-12
Author(s):  
Hari Pada Seal ◽  
Mohammad Amdad Ali ◽  
Md Usuf Ali ◽  
Mosammat Hasina Akhter ◽  
Fowzia Sultana
Keyword(s):  
Thin Layer ◽  
Chromatographic Analysis ◽  
Mixed Solvent ◽  
Solvent System ◽  
Bixa Orellana ◽  
Solvent Extraction Method ◽  
Solvent Systems ◽  
Carrier Solvent ◽  
Thin Layer Chromatographic Analysis ◽  
Layer Chromatography

This article describes a simple solvent extraction method for the extraction of colorants from the three morphotypes such as, (Morphotype-1 (M1), Morphotype-2 (M2), and Morphotype- 3 (M3) of Annatto (Bixa orellana L.) seeds, and their separation, vivid, and qualitative demonstration by thin-layer chromatography. Several solvent systems (hexane, chloroform, acetone, ethanol, and a mixed-solvent having composition of CHCl3/C2H5OH/CH3COOH (80:2:1)) were applied for extraction of colored components. It was observed that a large portion of colorants was extracted by chloroform. Its effluent was deep brick red in color and transparent. Furthermore, various carrier solvent systems (Benzene-Ethyl acetate) were used to separate the components from the extracts. Carrier solvent system with the ratio of 7:3 was found as superior solvent for chloroform extracts. Three colored-spots were observed for all morphotypes. Among them, the first one was yellow colored having very low polarity and the second and third spots were both redbrick colored having medium and higher polarity respectively. In addition, for M1 no colorless-spot was observed in low and medium polar systems, revealing that the amount of wax and gum were minimum in the extract and superior morphotype among the three. DOI: http://dx.doi.org/10.3329/ijarit.v2i1.13987 Int. J. Agril. Res. Innov. & Tech. 2 (1): 7-12, June, 2012

scholarly journals Validated high performance thin layer chromatographic analysis of leaves and flower extracts of

2022 ◽  
Vol 8 (4) ◽  
pp. 270-279
Author(s):  
Anup K Chakraborty ◽  
Harshita Tripathi ◽  
Sarita Karole ◽  
Kavita R Loksh
Keyword(s):  
Thin Layer ◽  
Silica Gel ◽  
Chromatographic Analysis ◽  
High Performance ◽  
Chemotherapeutic Agents ◽  
Simultaneous Estimation ◽  
Ich Guidelines ◽  
Tlc Plates ◽  
Thin Layer Chromatographic Analysis ◽  
Hptlc Method

is a significant therapeutic plant has a place with family apocynaceae contains in excess of 70 distinct sorts of chemotherapeutic agents and alkaloids which help in treating different illnesses. For the most part, it is known as Vincarosea, Ammocallisrosea and Lochnerarosea. There are numerous or more than 400 alkaloids present in plant, which are used as flavor, agrochemicals, pharmaceuticals, fragrance, ingredients, food addictives, and pesticides. To develop a validated high performance thin layer chromatographic method for the analysis of leaves and flower extracts of Sample solutions were applied onto the plates with automatic TLC sampler Linomat V (Camag, Muttenz, Switzerland) and were controlled by WinCATS software. Plates were developed in 10 x 10cm twin trough glass chamber (Camag, Muttenz, Switzerland). A CAMAG TLC scanner was used for scanning the TLC plates. Pre-coated silica gel aluminium plates 60F254. For vincristine, simultaneous estimation of vincristine was performed by HPTLC on a silica gel plate using toluene-methanol-diethylamine (8.75: 0.75: 0.5, v/v/v) as the mobile phase. The method was validated as per the ICH guidelines. The Rf value was found to be 0.76 for flower and 0.80 for leaves at 250 nm which shows the presence of vincristin in . In this research paper, a validated HPTLC Method has been developed for the analysis of leaves and flower extracts

scholarly journals Determination of Polysorbate 60 in Salad Dressings by Colorimetric and Thin Layer Chromatographic Techniques

1982 ◽  
Vol 65 (1) ◽  
pp. 162-165
Author(s):  
Daniel H Daniels ◽  
Charles R Warner ◽  
Sami Selim
Keyword(s):  
Thin Layer ◽  
Silica Gel ◽  
Column Chromatography ◽  
Chromatographic Analysis ◽  
Methylene Chloride ◽  
Chromatographic Techniques ◽  
Methylene Chloride Extract ◽  
Dragendorff Reagent ◽  
Thin Layer Chromatographic Analysis

Abstract Methods are described for the quantitation and verification of polysorbate 60 (PS 60) in salad dressing. The sample is partitioned between methylene chloride and water. The methylene chloride extract is further treated by silica gel column chromatography. The isolated PS 60 is complexed with ammonium cobaltothiocyanate and determined spectrophotometrically at 620 nm. Additional evidence indicating the presence of PS 60 is obtained from thin layer chromatographic analysis using the modified Dragendorff reagent for visualization of spots.

DEVELOPMENT OF HPTLC METHOD FOR THE ESTIMATION OF ONDANSETRON AND RANITIDINE IN COMBINED DOSAGE FORM

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (12) ◽  
pp. 42-48
Author(s):  
P. J. Patel ◽  
◽  
D. A Shah ◽  
F. A. Mehta ◽  
U. K. Chhalotiya
Keyword(s):  
Thin Layer ◽  
Stationary Phase ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
High Performance ◽  
Dosage Form ◽  
Solvent System ◽  
Rf Values ◽  
Layer Chromatography ◽  
Hptlc Method

A simple, sensitive and precise high performance thin layer chromatographic (HPTLC)method has been developed for the estimation of ondansetron (OND) and ranitidine (RAN) in combination. The method was employed on thin layer chromatography (TLC) and aluminium plates were precoated with silica gel 60 F254 as the stationary phase, while the solvent system was methanol. The Rf values were observed to be 0.5 ± 0.02, and 0.3 ± 0.02 for OND and RAN, respectively. The separated spots were densitometrically analyzed in absorbance mode at 299 nm. This method was linear in the range of 25-300 ng/band for OND and 50-600 ng/band for RAN. The limits of detection for OND and RAN were found to be 3.47 and 1.83 ng/band, respectively. The limits of quantification for OND and RAN were found to be 10.53 and 5.55 ng/band, respectively. The proposed method was validated with respect to linearity, accuracy, precision and robustness. The method was successfully applied to the estimation of OND and RAN in combined dosage form.

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