High-pressure liquid chromatography of polyhydric alcohols on silica gel columns

1974 ◽  
Vol 100 (1) ◽  
pp. 233-235 ◽  
Author(s):  
Greg P. Belue
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Silica Gel ◽  
High Pressure Liquid Chromatography ◽  
Polyhydric Alcohols

Trace Analysis of Zearalenone and/or Zearalanol in Animal Chow by High Pressure Liquid Chromatography and Gas-Liquid Chromatography

1977 ◽  
Vol 60 (2) ◽  
pp. 272-278 ◽  
Author(s):  
Claude L Holder ◽  
Charles R Nony ◽  
Malcolm C Bowman
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Silica Gel ◽  
High Pressure Liquid Chromatography ◽  
Additional Data ◽  
Gas Liquid Chromatography ◽  
Step Procedure ◽  
Rf Values ◽  
Solvent Systems ◽  
Layer Chromatography

Abstract An analytical method is described for determining residues of the estrogens zearalenone and/or zearalanol in animal chow at levels as low as 10 ppb. The chow is extracted with methanol and cleaned up by a 3-step procedure employing a Sephadex LH-20 column, liquid-liquid partitioning at pH 13 and 8.3, and a silica gel column. Residues of the 2 compounds, separated on silica gel, are assayed by using high pressure liquid chromatography with ultraviolet detection. Additional data are also included concerning p-values of the compounds in several solvent systems, Rf values from thin layer chromatography with 9 solvent systems, solubilities in 3 solvents, and a procedure for preparing their pentafluoropropionyl derivatives for analysis by electron capture gas-liquid chromatography.

High-Pressure Liquid Chromatography of Benzo (a) pyrene and Benzo(ghi)perylene in Oil-Contaminated Shellfish

1976 ◽  
Vol 59 (5) ◽  
pp. 989-992 ◽  
Author(s):  
Humberto Guerrero ◽  
Edward R Biehl ◽  
Charles T Kenner
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Petroleum Ether ◽  
Silica Gel ◽  
High Pressure Liquid Chromatography ◽  
High Pressure Liquid Chromatographic ◽  
Chromatographic Procedure ◽  
Liquid Chromatographic ◽  
Polynuclear Aromatics

Abstract A high-pressure liquid chromatographic procedure is described for the determination of benzo (a) pyrene and benzo(ghi)perylene. These polynuclear aromatics are extracted with acetonitrile and partitioned into petroleum ether, the petroleum ether is removed, and the residue is saponified. The compounds are purified and isolated by passing the residue through a silica gel column and a high-pressure liquid chromatographic column, and detected by their ultraviolet absorption. Recoveries of standards through the procedure averaged 104%.

An improved synthesis of m-diazirinophenol

1984 ◽  
Vol 62 (9) ◽  
pp. 1767-1771 ◽  
Author(s):  
Pierre Leblanc ◽  
Gerhard E. Gerber
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Silica Gel ◽  
High Pressure Liquid Chromatography ◽  
Good Yield ◽  
Reverse Phase ◽  
Oxidation Step ◽  
Work Up

The published synthesis of phenolic diazirine was shown to result in products which were chlorinated during the hypochlorite oxidation while the desired unchlorinated product was lost during the usual work-up. A superior synthesis is described: inclusion of pyridine during the oxidation step prevents the chlorination; the desired volatile diazirine was isolated in good yield using silica gel and reverse phase high pressure liquid chromatography.

Ball-Point Pen Ink Examination by High Pressure Liquid Chromatography

1977 ◽  
Vol 60 (3) ◽  
pp. 613-618 ◽  
Author(s):  
Lawrence F Colwell ◽  
Barry L Karger
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Silica Gel ◽  
High Pressure Liquid Chromatography ◽  
Mobile Phase ◽  
Syringe Needle ◽  
Ball Point

Abstract High pressure liquid chromatography was applied to the analysis of ball-point pen inks. A written line was sampled by extracting 10 plugs punched with a syringe needle. All separations were performed on a 25 cm silica gel (10 μm) column with a mobile phase of 2% formamide in methanol. Differences in ball-point pen inks based on different dyes or relative dye amounts were easily established. Of special note was the ability to distinguish inks based on the ratio of vehicle (resins, viscosity adjusters, etc.) to dye or on the types of vehicles employed.

Sensitive Silica Gel-Packed Flowcell for Fluorometric Detection of Aflatoxins by High Pressure Liquid Chromatography

1977 ◽  
Vol 60 (3) ◽  
pp. 583-589 ◽  
Author(s):  
Thavil Panalaks ◽  
Peter M Scott
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Life Expectancy ◽  
Silica Gel ◽  
High Pressure Liquid Chromatography ◽  
Peanut Butter ◽  
Peak Height ◽  
Fluorometric Detection ◽  
Cleanup Procedure ◽  
The Relationship

Abstract Aflatoxins B1, B2, G1, and G2 were quantitatively detected by High pressure liquid chromatography on a 5 μm Lichrosorb column, using a Lichrosorb-packed flowcell in the fluorometric detector. The relationship between peak height and the amount injected was linear only up to about 2 ng but showed a linear log-log relationship. Methods for constructing and packing the flowcell are given. A guard column and venting valve were used to minimize deterioration of the analytical column and the adsorbent-packed flowcell. The method was applied to a peanut butter extract, although with the cleanup procedure used, the life expectancy of the flowcell is limited.

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