Gel permeation, ion-exchange and reversed-phase columns for separation of metallothioneins by high-performance liquid chromatograph—atomic absorption spectrophotometry

1983 ◽  
Vol 281 ◽  
pp. 159-166 ◽  
Author(s):  
Kazuo T. Suzuki ◽  
Hiroyuki Sunaga ◽  
Yasunobu Aoki ◽  
Mitsuru Yamamura
Keyword(s):  
Ion Exchange ◽  
Atomic Absorption ◽  
High Performance ◽  
Atomic Absorption Spectrophotometry ◽  
Reversed Phase ◽  
High Performance Liquid Chromatograph ◽  
Liquid Chromatograph ◽  
Absorption Spectrophotometry ◽  
Gel Permeation

Use of a flow-injection sample manipulator as an interface between a "high-performance" liquid chromatograph and an atomic absorption spectrophotometer.

1981 ◽  
Vol 27 (9) ◽  
pp. 1546-1550 ◽  
Author(s):  
B W Renoe ◽  
C E Shideler ◽  
J Savory
Keyword(s):  
Atomic Absorption ◽  
Flow Injection ◽  
Metal Binding ◽  
High Performance ◽  
Atomic Absorption Spectrophotometry ◽  
Clinical Samples ◽  
High Performance Liquid Chromatograph ◽  
Liquid Chromatograph ◽  
Flame Atomic Absorption ◽  
Column Eluate

Abstract We describe an integrated, molecular-absorbance, atomic absorption instrument for studying metal/ligand binding in clinical samples. For an interface between the "high-performance" liquid chromatograph and the atomic absorption instrument we used a flow-injection sample manipulator, thus allowing both the chromatograph and the atomic absorption detector to operate at their separate optimum conditions. After specimen separation with a gel permeation column, we measured the molecular components of the column eluate by molecular absorbance spectrometry and the atomic components (calcium and magnesium) by flame atomic absorption spectrophotometry. This instrument system is capable of separating and analyzing multiple components within 20 min of injection of the sample on the column. The chromatograms presented demonstrate the utility of the system for investigating metal binding to a variety of ligands in clinical samples.

Determination of Free and Bound Cd, Zn, Cu, and Ag Ions in Lobster (Homarus americanus) Digestive Gland Extracts by Gel Chromatography Followed by Atomic Absorption Spectrophotometry and Polarography

1993 ◽  
Vol 76 (4) ◽  
pp. 794-798 ◽  
Author(s):  
Chiu L Chou ◽  
John F Uthe ◽  
Robert D Guy
Keyword(s):  
Molecular Weight ◽  
Metal Ions ◽  
Atomic Absorption ◽  
Gel Permeation Chromatography ◽  
Weight Fraction ◽  
Atomic Absorption Spectrophotometry ◽  
Divalent Metal ◽  
Divalent Metal Ions ◽  
Absorption Spectrophotometry ◽  
Gel Permeation

Abstract Gel-permeation chromatography followed by atomic absorption spectrophotometric and polarographic analyses were used to measure free and bound divalent metal ions in lobster digestive gland extracts prepared with or without oc-toluenesulfonyl fluoride, a protease inhibitor. Chromatography on Sephadex G-50 or G-100 yielded 3 UV-absorbing peaks, which corresponded, respectively, to the void volume, a medium molecular weight fraction, and a low molecular weight fraction that contained free divalent metal ions. In protease-inhibited extracts, only Zn+2 was found, whereas Cd, Cu, and Ag were bound in high and medium molecular weight fractions. Cd+2 and Zn+2 were rapidly released from their bound forms in the absence of a protease inhibitor, and their presence was confirmed by polarography with EDTA. Gel-permeation chromatography coupled with atomic absorption spectrophotometry offers a rapid method for following changes in the concentrations of bound and free metal ions during processing of shellfishbased foodstuffs.

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