scholarly journals Content determination of the flavonoids in the different parts and different species of Abelmoschus esculentus L. by reversed phase-high performance liquid chromatograph and colorimetric method

2014 ◽  
Vol 10 (39) ◽  
pp. 278 ◽  
Author(s):  
Yin Lin ◽  
Min-feng Lu ◽  
Hai-bing Liao ◽  
Yu-xian Li ◽  
Wei Han ◽  
...  
Keyword(s):  
High Performance ◽  
Colorimetric Method ◽  
Reversed Phase ◽  
Abelmoschus Esculentus ◽  
High Performance Liquid Chromatograph ◽  
Liquid Chromatograph ◽  
Content Determination ◽  
Different Parts

Efficient high performance liquid chromatograph/ultraviolet method for determination of diclofenac and 4′-hydroxydiclofenac in rat serum

2006 ◽  
Vol 830 (2) ◽  
pp. 231-237 ◽  
Author(s):  
Lata Kaphalia ◽  
Bhupendra S. Kaphalia ◽  
Santosh Kumar ◽  
Mary F. Kanz ◽  
Mary Treinen-Moslen
Keyword(s):  
High Performance ◽  
High Performance Liquid Chromatograph ◽  
Rat Serum ◽  
Liquid Chromatograph

Determination of uric acid in grain using HPLC

2021 ◽  
pp. 74-77
Author(s):  
Артём Валерьевич Яицких ◽  
Геннадий Алексеевич Закладной ◽  
Дмитрий Сергеевич Степаненко
Keyword(s):  
Uric Acid ◽  
Extraction Method ◽  
High Performance ◽  
Reversed Phase ◽  
Calibration Graph ◽  
Improved Method ◽  
Liquid Chromatograph ◽  
Yield Time ◽  
Grain Contamination

В данной статье показана возможность улучшения определения мочевой кислоты методом высокоэффективной жидкостной хроматографии (ВЭЖХ), с помощью увеличения растворимости ее в 1 %-ном растворе ацетата натрия, повышения удержания мочевой кислоты и тем самым изменения времени выхода, что позволяет повысить точность анализа. Проведено сравнение градуировочных растворов и опытных образцов зерна зараженных вредителями хлебных запасов. В процессе исследования был опробован метод для определения и идентификации мочевой кислоты как одного из загрязняющих зерно веществ с помощью ВЭЖХ в обращенной фазе. Экспериментально опробована и усовершенствована методика анализа мочевой кислоты в зерне с использованием жидкостного хроматографа «Стайер». Описаны оборудование и материалы для ВЭЖХ, условия хроматографического разделения и детектирования, построения калибровочного графика, экстракции, включая методику экстракции, сходимости результатов при экстракции и введении экстракта в хроматограф, а также порядок и расчет измерений. Экспериментально показано, что усовершенствованная методика с применением ВЭЖХ позволяет использовать ее для проведения дальнейших исследований зависимости содержания мочевой кислоты от величины загрязнения зерна насекомыми. This article shows the possibility of improving the determination of uric acid by high-performance liquid chromatography (HPLC), by increasing its solubility in a 1 % solution of sodium acetate, increasing the retention of uric acid, and thereby changing the yield time, which allow to improve the accuracy of the analysis, a comparison of calibration solutions and experimental grain samples of pest-infected bread stocks have been carried out. During the research course a method for the determination and identification of uric acid has been tested as one of the grain polluting substance using HPLC in the reversed phase. The method of uric acid in grain analysis using a liquid chromatograph «Stayer» has been experimentally tested and improved. The equipment and materials for HPLC, the conditions of chromatographic separation and detection, the construction of a calibration graph, extraction, including the extraction method, the convergence of the results during extraction and the introduction of the extract into the chromatograph, as well as the course and calculation of measurements have been described. It has been experimentally shown that the improved method with HPLC allows to use it for further research of the uric acid content dependence on the amount of grain contamination by insects.

Determination of 8-methoxypsoralen in suction-blister fluid and serum by liquid chromatography.

1980 ◽  
Vol 26 (13) ◽  
pp. 1825-1828 ◽  
Author(s):  
M J Herfst ◽  
P M Edelbroek ◽  
F A de Wolff
Keyword(s):  
High Performance ◽  
Internal Standard ◽  
Reversed Phase ◽  
Pharmacokinetic Studies ◽  
Blister Fluid ◽  
Liquid Chromatograph ◽  
Suction Blister ◽  
Drug Concentrations ◽  
Suction Blister Fluid

Abstract A method is described for determination of 8-methoxypsoralen to 0.2 mL of suction-blister fluid or 1 mL of serum from psoriatic patients being treated with this drug. The drug is extracted from the biological matrix at pH 9.0 with a mixture of dichloromethane and light petroleum ether. 5-Methoxypsoralen is used as internal standard. Separation and quantitation are performed on a “high-performance” liquid chromatograph with use of an RP 18 reversed-phase column and detection at 245 nm. Accuracy and precision are good. Some benzodiazepines and their metabolites interfere. The lowest detectable concentration is 10 microgram/L, which means that the method is sufficiently sensitive to measure the drug concentrations in serum and suction-blister fluid for pharmacokinetic studies in patients being treated with a therapeutic dosage.

Determination of thiamin and thiamin phosphate esters in blood by liquid chromatography with post-column derivatization.

1983 ◽  
Vol 29 (12) ◽  
pp. 2073-2075 ◽  
Author(s):  
M Kimura ◽  
Y Itokawa
Keyword(s):  
High Performance ◽  
Sodium Hydroxide Solution ◽  
Potassium Ferricyanide ◽  
Phosphate Esters ◽  
High Performance Liquid Chromatograph ◽  
Liquid Chromatograph ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic ◽  
Thiamin Pyrophosphate

Abstract We demonstrate a liquid-chromatographic method involving post-column derivatization for determining the concentration of thiamin and its phosphate esters in human blood. Blood, erythrocytes, or plasma is deproteinized and centrifuged. Aliquots of the samples are applied to a mu Bondapak C18 column attached to a "high-performance" liquid chromatograph. Addition of potassium ferricyanide/sodium hydroxide solution to the column effluent with a proportioning pump converts thiamin phosphates into fluorophores, the intensities of which are measured with a spectrofluorophotometer. Thiamin, thiamin monophosphate, thiamin pyrophosphate, and thiamin triphosphate eluted as single peaks; no coeluting substances were detected. Thiamin pyrophosphate was the ester present in greatest concentration, followed by thiamin triphosphate; thiamin monophosphate and thiamin were present in slight amounts. This method allows easy determination of thiamin and its phosphate esters in 0.1 mL of blood.

scholarly journals Simultaneous Determination of Four Marker Compounds in Lobelia chinensis Lour. Extract by HPLC-PDA

2021 ◽  
Vol 11 (24) ◽  
pp. 12080
Author(s):  
Beom-Geun Jo ◽  
Young-Hun Park ◽  
Ki Hyun Kim ◽  
Su-Nam Kim ◽  
Min Hye Yang
Keyword(s):  
Formic Acid ◽  
High Performance ◽  
Reverse Phase ◽  
High Performance Liquid Chromatograph ◽  
Liquid Chromatograph ◽  
Snake Bites ◽  
Antiviral Activities ◽  
Photodiode Array ◽  
Marker Compounds

Lobelia chinensis Lour. (L. chinensis) has traditionally been used as a treatment for snake bites, high fever, jaundice, edema, and diarrhea, and modern studies have reported its anti-inflammatory, antioxidant, and antiviral activities. L. chinensis contains various compounds, such as flavonoids and coumarins, and its flavonoid components have been identified in many studies. In this study, a high-performance liquid chromatograph equipped with a photodiode array (PDA) detector and an Aegispak C18-L reverse-phase column (4.6 mm × 250 mm i.d., 5 μm) was used to simultaneously analyze four marker components in L. chinensis for standardization purposes. HPLC-PDA (detection at 340 nm), performed using a 0.1% formic acid-water/0.1% formic acid-acetonitrile gradient, separated the four marker compounds: luteolin-7-O-β-d-glucuronopyranosyl (1→2)-O-β-d-glucuronopyranoside, clerodendrin, chrysoeriol-7-O-diglucuronide, and diosmin. The developed analytical method showed excellent linearity values (r2 > 0.9991), limits of detection (LODs: 0.376–2.152 μg/mL), limits of quantification (LOQs: 1.147–6.521 μg/mL), intra- and inter-day precisions (RSD < 1.96%), and analyte recoveries (96.83–127.07%; RSD < 1.73%); thus, it was found to be suitable for the simultaneous analysis of these four marker compounds in L. chinensis.

Liquid-chromatographic determination of zomepirac in serum and plasma.

1982 ◽  
Vol 28 (3) ◽  
pp. 481-484 ◽  
Author(s):  
C L Welch ◽  
T M Annesley ◽  
H S Luthra ◽  
T P Moyer
Keyword(s):  
High Performance ◽  
Limit Of Detection ◽  
Peak Plasma ◽  
Peak Plasma Concentration ◽  
Internal Standard ◽  
Chromatographic Determination ◽  
Reversed Phase ◽  
Liquid Chromatograph ◽  
Days Of Therapy

Abstract We describe a determination of zomepirac concentration in plasma and serum by reversed-phase "high-performance" liquid chromatography. The assay requires 1.0 mL of sample and involves diethyl ether extraction of zomepirac from an acidified sample, followed by concentration and injection into a liquid chromatograph. The column effluent is monitored at 330 nm. Retention times of zomepirac and the internal standard (tolmetin) are 3.8 and 2.7 min, respectively. The lower limit of detection for zomepirac in serum or plasma is 0.05 mg/L. Within-day precision (CV) of analysis in plasma or serum with zomepirac added (0.1-10.0 mg/L) ranged from 1.4 to 6.7%; between-day CV varied from 1.4 to 7.5%. Analytical recovery of zomepirac (1.0 mg/L) from serum and plasma was 77.6 (SD 3.5)% and 80.4 (SD 5.2)%, respectively. Numerous commonly coadministered drugs did not interfere. The elimination half-life of the drug was 1.8 h, and the peak plasma concentration ranged from 1.1 to 2.4 mg/L. Peak and trough concentrations measured throughout five days of therapy imply no accumulation.

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