Evaluation of ammonium acetate as a volatile buffer for high-performance hydrophobic-interaction chromatography

1990 ◽  
Vol 515 ◽  
pp. 279-283 ◽  
Author(s):  
Tadao Konishi ◽  
Masafumi Kamada ◽  
Hiroshi Nakamura
1999 ◽  
Vol 22 (13) ◽  
pp. 1965-1974 ◽  
Author(s):  
Yosuke Yamasaki ◽  
Kanae Uchida ◽  
Hiroshi Tomizawa ◽  
Osamu Ishikawa ◽  
Kuniyo Inouye

1988 ◽  
Vol 252 (3) ◽  
pp. 753-758 ◽  
Author(s):  
C P Quinn ◽  
C C Shone ◽  
P C B Turnbull ◽  
J Melling

A procedure has been developed for purification of the tripartite anthrax-toxin components. This involves sequential high-performance anion-exchange, gel-filtration and hydrophobic-interaction chromatography. From an initial culture volume of 15 litres, typical yields of 8 mg of protective antigen, 13 mg of lethal factor and 7 mg of oedema factor are produced to higher degrees of purity than have previously been achieved by conventional chromatographic techniques.


Sign in / Sign up

Export Citation Format

Share Document