Separation and quantitation of ribulose-1,5-bisphosphate carboxylase-oxygenase in spinach leaves by high-performance hydrophobic-interaction chromatography

1987 ◽  
Vol 395 ◽  
pp. 523-529 ◽  
Author(s):  
Danilo Corradini ◽  
Maria Teresa Giardi ◽  
Angelo Massacci
Keyword(s):  
Hydrophobic Interaction ◽  
High Performance ◽  
Hydrophobic Interaction Chromatography ◽  
Bisphosphate Carboxylase ◽  
Spinach Leaves

SEPARATION OF AMINO ACIDS AND PEPTIDES BY HIGH PERFORMANCE HYDROPHOBIC INTERACTION CHROMATOGRAPHY

1999 ◽  
Vol 22 (13) ◽  
pp. 1965-1974 ◽  
Author(s):  
Yosuke Yamasaki ◽  
Kanae Uchida ◽  
Hiroshi Tomizawa ◽  
Osamu Ishikawa ◽  
Kuniyo Inouye
Keyword(s):  
Amino Acids ◽  
Hydrophobic Interaction ◽  
High Performance ◽  
Hydrophobic Interaction Chromatography

scholarly journals Purification of anthrax-toxin components by high-performance anion-exchange, gel-filtration and hydrophobic-interaction chromatography

1988 ◽  
Vol 252 (3) ◽  
pp. 753-758 ◽  
Author(s):  
C P Quinn ◽  
C C Shone ◽  
P C B Turnbull ◽  
J Melling
Keyword(s):  
Anion Exchange ◽  
Hydrophobic Interaction ◽  
High Performance ◽  
Protective Antigen ◽  
Lethal Factor ◽  
Gel Filtration ◽  
Hydrophobic Interaction Chromatography ◽  
Anthrax Toxin ◽  
Culture Volume ◽  
Initial Culture

A procedure has been developed for purification of the tripartite anthrax-toxin components. This involves sequential high-performance anion-exchange, gel-filtration and hydrophobic-interaction chromatography. From an initial culture volume of 15 litres, typical yields of 8 mg of protective antigen, 13 mg of lethal factor and 7 mg of oedema factor are produced to higher degrees of purity than have previously been achieved by conventional chromatographic techniques.

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