Effects of alcohol mobile-phase modifiers on the structure and chiral selectivity of amylose tris(3,5-dimethylphenylcarbamate) chiral stationary phase

2003 ◽  
Vol 1015 (1-2) ◽  
pp. 99-110 ◽  
Author(s):  
Tao Wang ◽  
Robert M Wenslow
Keyword(s):  
Stationary Phase ◽  
Mobile Phase ◽  
Chiral Stationary Phase ◽  
Mobile Phase Modifiers ◽  
Chiral Selectivity

Quinine-Based Zwitterionic Chiral Stationary Phase as a Complementary Tool for Peptide Analysis: Mobile Phase Effects on Enantio- and Stereoselectivity of Underivatized Oligopeptides

Chirality ◽  
2015 ◽  
Vol 28 (1) ◽  
pp. 5-16 ◽  
Author(s):  
Federica Ianni ◽  
Roccaldo Sardella ◽  
Andrea Carotti ◽  
Benedetto Natalini ◽  
Wolfgang Lindner ◽  
...  
Keyword(s):  
Stationary Phase ◽  
Mobile Phase ◽  
Chiral Stationary Phase ◽  
Peptide Analysis ◽  
Mobile Phase Effects

A High-Throughput Screening of N-Carbobenzoxy-L-Tryptophan Imprinted Polymers and their Application for Monolithic Chiral Stationary Phase

2012 ◽  
Vol 535-537 ◽  
pp. 1525-1528 ◽  
Author(s):  
Jian Qi ◽  
Li Guo ◽  
Hai Feng Sang
Keyword(s):  
Stationary Phase ◽  
Molecularly Imprinted Polymers ◽  
High Throughput Screening ◽  
Chiral Stationary Phase ◽  
Binding Capacity ◽  
Low Cost ◽  
Small Scale ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Chiral Selectivity

Molecular imprinting is a technique to prepare polymers with predetermined selectivity, specific recognition and predesigned affinity to a desired molecule. The stability and low cost of molecularly imprinted polymers (MIPs) render them attractive for a broad range of applications. Currently, MIP technique has been widely used in chiral separation. In this study, a series of molecularly imprinted polymers for N-Carbobenzoxy-L-tryptophan (N-Cbz-L-Trp) synthesized in different conditions were prepared in a small scale to simulate the monolithic chiral stationary phases (CSPs) primarily. By coupling in situ processing and batch rebinding evaluation, the type of functional monomers, which likely to affect the chiral selectivity of MIPs, was investigated. It was found that a MIP comprising a mixture of functional monomer 4-vinylpyridine (4-VP) and porogen 1-dodecanol/toluene exhibited the highest binding capacity and chiral selectivity for N-Carbobenzoxy-L-tryptophan. Thereafter, the monolithic MIP synthesized in screened optimum condition is used as chiral stationary phase in HPLC, which shows favourable separating capacity.

Cellulose Tris(3,5-Dimethylphenylcarbamate) Regioselectively Bonded to Small Pore Silica Gel as Chiral Stationary Phase for HPLC

2011 ◽  
Vol 117-119 ◽  
pp. 1361-1364
Author(s):  
Yi Jun Zhang ◽  
Cai Xia Dong ◽  
Jun Chen ◽  
Run Qiang Liu
Keyword(s):  
Stationary Phase ◽  
Silica Gel ◽  
Mobile Phase ◽  
High Performance ◽  
Chiral Stationary Phase ◽  
High Performance Liquid Chromatographic ◽  
Hydroxyl Group ◽  
Cellulose Derivatives ◽  
Terephthaloyl Chloride ◽  
Small Pore

A bifunctional reagent of terephthaloyl chloride was initially adopted as a spacer reagent to prepare the bonded types of chiral stationary phase (CSP) with cellulose derivatives. (3,5-dimethylphenyl)carbamates of cellulose (CDMPC) regioselectively bonded to small pore (3-aminopropyl)silica gel (APS) were prepared with terephthaloyl chloride as a spacer at the 6-position of the primary hydroxyl group on the glucose unit of cellulose. Enantioseparations of five racemic samples are evaluated on the prepared CSP under normal-phase high-performance liquid chromatographic mode with hexane- isopropylalcohol as the mobile phase. The influence of flow rates and mobile phase compositions on the resolution were investigated. The prepared stationary phase was exhibited an effective chiral recognition.

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