Multidimensional counter-current chromatographic system and its application

1998 ◽  
Vol 803 (1-2) ◽  
pp. 298-301 ◽  
Author(s):  
Fuquan Yang ◽  
Jiang Quan ◽  
Tian You Zhang ◽  
Yoichiro Ito
Keyword(s):  
Chromatographic System ◽  
Counter Current

scholarly journals Development and Testing of a 4-Columns Periodic Counter-Current Chromatography System Based on Membrane Adsorbers

Separations ◽  
2019 ◽  
Vol 6 (4) ◽  
pp. 55 ◽  
Author(s):  
Chantal Brämer ◽  
Frank Lammers ◽  
Thomas Scheper ◽  
Sascha Beutel
Keyword(s):  
Mass Transport ◽  
Capacity Utilization ◽  
Cost Savings ◽  
Chromatographic System ◽  
Protein Mixture ◽  
Productivity Increase ◽  
Model Protein ◽  
Membrane Adsorber ◽  
Membrane Adsorbers ◽  
Counter Current

Continuous chromatography can surmount the disadvantages of batch chromatography like low productivities and extensive usage of consumables. In this work, a 4-column continuous chromatographic system based on the principle of periodic counter-current chromatography (PCCC) was developed and tested with a model protein mixture of BSA and lysozyme. The PCCC system was specially designed for membrane adsorbers as an alternative to conventional columns to facilitate the use of disposable process units and to further increase the productivity due to higher convective mass transport in the membrane adsorber. Membrane adsorber Sartobind® Q was used to continuously purify BSA from the protein mixture. The usage of PCCC led to an increased capacity utilization (here 20%) and higher space–time-yields, and thus to a remarkable productivity increase and cost savings.

FRACTIONATION AND ANALYSIS OF MONKEY PITUITARY GLANDS FOR POSTERIOR LOBE HORMONES

1962 ◽  
Vol 40 (2) ◽  
pp. 283-289 ◽  
Author(s):  
Darrell N. Ward ◽  
Earl F. Walborg ◽  
Harry S. Lipscomb ◽  
Roger Guillemin
Keyword(s):  
Amino Acid ◽  
Current Distribution ◽  
Arginine Vasopressin ◽  
Specific Activity ◽  
Small Scale ◽  
Posterior Lobe ◽  
Pharmacological Activities ◽  
Pituitary Glands ◽  
Counter Current ◽  
Counter Current Distribution

ABSTRACT Fractionation of monkey pituitary glands gave an oxytocin fraction in low yield which showed a counter-current distribution coefficient equivalent to that obtained with oxytocin from other species. Fractionation and chromatography of monkey vasopressin on carboxymethyl cellulose gave arginine-vasopressin of 60% purity, based on amino acid analysis and specific activity. Counter-current distribution on a small scale gave arginine-vasopressin of 89% purity. Reports by others that monkey pituitary glands contain arginine-vasopressin, based on pharmacological activities, are substantiated by the chemical data presented here.

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