scholarly journals Development and Testing of a 4-Columns Periodic Counter-Current Chromatography System Based on Membrane Adsorbers

Separations ◽  
2019 ◽  
Vol 6 (4) ◽  
pp. 55 ◽  
Author(s):  
Chantal Brämer ◽  
Frank Lammers ◽  
Thomas Scheper ◽  
Sascha Beutel
Keyword(s):  
Mass Transport ◽  
Capacity Utilization ◽  
Cost Savings ◽  
Chromatographic System ◽  
Protein Mixture ◽  
Productivity Increase ◽  
Model Protein ◽  
Membrane Adsorber ◽  
Membrane Adsorbers ◽  
Counter Current

Continuous chromatography can surmount the disadvantages of batch chromatography like low productivities and extensive usage of consumables. In this work, a 4-column continuous chromatographic system based on the principle of periodic counter-current chromatography (PCCC) was developed and tested with a model protein mixture of BSA and lysozyme. The PCCC system was specially designed for membrane adsorbers as an alternative to conventional columns to facilitate the use of disposable process units and to further increase the productivity due to higher convective mass transport in the membrane adsorber. Membrane adsorber Sartobind® Q was used to continuously purify BSA from the protein mixture. The usage of PCCC led to an increased capacity utilization (here 20%) and higher space–time-yields, and thus to a remarkable productivity increase and cost savings.

scholarly journals Membrane Adsorber for the Fast Purification of a Monoclonal Antibody Using Protein A Chromatography

Membranes ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 159 ◽  
Author(s):  
Chantal Brämer ◽  
Lisa Tünnermann ◽  
Alina Gonzalez Salcedo ◽  
Oscar-Werner Reif ◽  
Dörte Solle ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Continuous Process ◽  
Protein A ◽  
Capacity Utilization ◽  
Citrate Buffer ◽  
Cycle Times ◽  
Antibody Purification ◽  
Membrane Adsorber ◽  
Counter Current ◽  
Elution Step

Monoclonal antibodies are conquering the biopharmaceutical market because they can be used to treat a variety of diseases. Therefore, it is very important to establish robust and optimized processes for their production. In this article, the first step of chromatography (Protein A chromatography) in monoclonal antibody purification was optimized with a focus on the critical elution step. Therefore, different buffers (citrate, glycine, acetate) were tested for chromatographic performance and product quality. Membrane chromatography was evaluated because it promises high throughputs and short cycle times. The membrane adsorber Sartobind® Protein A 2 mL was used to accelerate the purification procedure and was further used to perform a continuous chromatographic run with a four-membrane adsorber-periodic counter-current chromatography (4MA-PCCC) system. It was found that citrate buffer at pH 3.5 and 0.15 M NaCl enabled the highest recovery of >95% and lowest total aggregate content of 0.26%. In the continuous process, the capacity utilization of the membrane adsorber was increased by 20%.

pH-Responsive magnetic nanospheres for the reversibly selective capture and release of glycoproteins

2017 ◽  
Vol 5 (6) ◽  
pp. 1236-1245 ◽  
Author(s):  
Qi Yang ◽  
Yue Zhu ◽  
Bin Luo ◽  
Fang Lan ◽  
Yao Wu ◽  
...  
Keyword(s):  
Biological Sample ◽  
High Selectivity ◽  
Stimuli Responsive ◽  
Ph Responsive ◽  
Protein Mixture ◽  
Magnetic Nanospheres ◽  
Protein Model ◽  
Pure Protein ◽  
Model Protein ◽  
Capture And Release

We present a pH-stimuli-responsive strategy to reversibly capture and release glycoproteins with high selectivity from a pure protein, model protein mixture and even a real biological sample.

Spatially discrete models of counter-current mass transport for application to the kidney

1978 ◽  
Vol 20 (4) ◽  
pp. 259-270 ◽  
Author(s):  
Gerald M. Saidel ◽  
Paramjit S. Chandhoke ◽  
Mark A. Knepper
Keyword(s):  
Mass Transport ◽  
Discrete Models ◽  
Current Mass ◽  
Counter Current

scholarly journals Economic Evaluation of Apple Harvest and In-Field Sorting Technology

2017 ◽  
Vol 60 (5) ◽  
pp. 1537-1550
Author(s):  
Zhao Zhang ◽  
Anand Kumar Pothula ◽  
Renfu Lu
Keyword(s):  
Economic Evaluation ◽  
Occupational Injuries ◽  
Cost Savings ◽  
Production Costs ◽  
Apple Orchards ◽  
Fresh Market ◽  
Apple Industry ◽  
Productivity Increase ◽  
One Machine ◽  
The U.S

Abstract. The U.S. apple industry, which generated more than $2.7 billion in revenue at the farm gate in 2013, is facing critical challenges with decreased availability of labor and increased labor and production costs. To address these challenges, a self-propelled apple harvest and in-field sorting machine is being developed in our laboratory. This article reports on the economic evaluation of this prototype machine by considering machine cost (annual ownership and operating costs), harvest productivity increase (including that due to decreased occupational injuries), and cost savings in postharvest storage and packing resulting from in-field sorting of fresh market quality apples from processing apples for both fresh apple growers and processing apple growers. The economic evaluation was conducted based on the assumptions that the machine increases harvest productivity by 43% to 63% and operates for 360 h during the harvest season. For fresh apple orchards with processing apple incidences of 5% to 15%, the net annual benefits that would accrue from owning one machine range from $13,500 to $78,400 when the machine price is between $100,000 and $160,000. For processing apple orchards with processing apple incidences of 80% to 90% and the same machine price range, the net annual benefits that would accrue from owning one machine range from $23,900 to $81,700. Overall, the benefits gained from in-field sorting outweigh those from the harvest productivity increase, and integration of the harvest-assist and in-field sorting functions is more beneficial to apple growers. This technology will help the U.S. apple industry improve labor productivity and reduce production costs, and thus it looks promising for commercialization. Keywords: Apples, Economic evaluation, Harvest-assist, In-field sorting, Machinery system, Occupational injuries.

Multidimensional counter-current chromatographic system and its application

1998 ◽  
Vol 803 (1-2) ◽  
pp. 298-301 ◽  
Author(s):  
Fuquan Yang ◽  
Jiang Quan ◽  
Tian You Zhang ◽  
Yoichiro Ito
Keyword(s):  
Chromatographic System ◽  
Counter Current
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