Background/Aim. Periodontal disease affects gingival tissue and supporting
apparatus of the teeth leading to its decay. The aim of this study was to
highlight and precisely determine histological changes in the gum tissue.
Methods. Gingival biopsy samples from 53 healthy and parodontopathy-affected
patients were used. Clinical staging of the disease was performed. Tissue
specimens were fixed and routinely processed. Sections, 5 ?m thin, were
stained with hematoxylin and eosin, histochemical Van-Gieson for the collagen
content, Spicer method for mast-cells and immunochemical method with
anti-CD68 and anti-CD38 for the labelling of the macrophages and
plasma-cells. Morphometric analysis was performed by a M42 test system.
Results. While the disease advanced, collagen and fibroblast volume density
decreased almost twice in the severe cases compared to the control ones, but
a significant variation was observed within the investigated groups. The
mast-cell number increased nearly two times, while the macrophage content was
up to three times higher in severe parodontopathy than in healthy gingival
tissue. However, the relative proportion of these cells stayed around 6% in
all cases. Plasma-cells had the most prominent increase in the number (over 8
times) compared to the control, but again, a variation within investigated
groups was very high. Conclusion. Gingival tissue destruction caused by
inflammatory process leads to significant changes in collagen density and
population of resident connective tissue cells. Although inflammatory cells
dominated with the disease advancing, a high variation within the same
investigated groups suggests fluctuation of the pathological process. <br><br><font color="red"><b> This article has been corrected. Link to the correction <u><a href="http://dx.doi.org/10.2298/VSP1704391E">10.2298/VSP1704391E</a><u></b></font>
Measurement of two plasma triglyceride lipases by an immunochemical method: studies in patients with hypertriglyceridemia
