Factors influencing Lp[a]- particle size as determined by gradient gel electrophoresis.

Abstract Background: Our study seeks to clarify the extent of differences in analytical results, from a clinical perspective, among 4 leading technologies currently used in clinical reference laboratories for the analysis of LDL subfractions: gradient gel electrophoresis (GGE), ultracentrifugation–vertical auto profile (VAP), nuclear magnetic resonance (NMR), and tube gel electrophoresis (TGE). Methods: We collected 4 simultaneous blood samples from 40 persons (30 males and 10 females) to determine LDL subclasses in 4 different clinical reference laboratories using different methods for analysis. LDL subfractions were assessed according to LDL particle size and the results categorized according to LDL phenotype. We compared results obtained from the different technologies. Results: We observed substantial heterogeneity of results and interpretations among the 4 methods. Complete agreement among methods with respect to LDL subclass phenotyping occurred in only 8% (n = 3) of the persons studied. NMR and GGE agreed most frequently at 70% (n = 28), whereas VAP matched least often. Conclusions: As measurement of LDL subclasses becomes increasingly important, standardization of methods is needed. Variation among currently available methods renders them unreliable and limits their clinical usefulness.

Download Full-text

Comparison between Gradient Gel Electrophoresis and Nuclear Magnetic Resonance Spectroscopy in Estimating Coronary Heart Disease Risk Associated with LDL and HDL Particle Size

Clinical Chemistry ◽

10.1373/clinchem.2009.140939 ◽

2010 ◽

Vol 56 (5) ◽

pp. 789-798 ◽

Cited By ~ 27

Author(s):

Benoit J Arsenault ◽

Isabelle Lemieux ◽

Jean-Pierre Després ◽

Nicholas J Wareham ◽

Erik SG Stroes ◽

...

Keyword(s):

Nuclear Magnetic Resonance ◽

Coronary Heart Disease ◽

Particle Size ◽

Heart Disease ◽

Magnetic Resonance ◽

Gel Electrophoresis ◽

Ldl Size ◽

Chd Risk ◽

Gradient Gel Electrophoresis ◽

Nuclear Magnetic

Abstract Background: Gradient gel electrophoresis (GGE) and nuclear magnetic resonance (NMR) spectroscopy are both widely accepted methods for measuring LDL and HDL particle size. However, whether or not GGE- or NMR-measured LDL or HDL particle size predicts coronary heart disease (CHD) risk to a similar extent is currently unknown. Methods: We used GGE and NMR to measure LDL and HDL particle size in a nested case-control study of 1025 incident cases of CHD and 1915 controls from the EPIC (European Prospective Investigation into Cancer and Nutrition)-Norfolk study. The study sample included apparently healthy men and women age 45–79 years followed for an average of 6 years. Results: Pearson correlation coefficients showed that the overall agreement between NMR and GGE was better for the measurement of HDL size (r = 0.78) than for LDL size (r = 0.47). The odds ratio for future CHD among participants in the bottom tertile of LDL size (smallest LDL particles) was 1.35 (95% CI, 1.12–1.63) for GGE and 1.74 (1.41–2.15) for NMR. For HDL size, these respective odds ratios were 1.41 (1.16–1.72) and 1.85 (1.47–2.32). After adjustment for potential confounders, the relationship between small LDL or HDL particles and CHD was no longer significant, irrespective of the method. Conclusions: In this prospective population study, we found that the relationships between NMR-measured LDL and HDL sizes and CHD risk were slightly higher than those obtained with GGE.

Download Full-text

Determination of Low Density Lipoprotein Particle Size by Polyacrylamide Gradient Gel Electrophoresis in Patients with Coronary Artery Stenosis

Laboratory Medicine ◽

10.1309/lmr1mwbggi3kxzei ◽

2010 ◽

Vol 41 (3) ◽

pp. 164-166 ◽

Cited By ~ 2

Author(s):

Romina Karimzadeh Ghassab ◽

Ladan Hosseini Gohari ◽

Mohsen Firoozray ◽

Morteza Norouzi Yegane

Keyword(s):

Particle Size ◽

Coronary Artery ◽

Gel Electrophoresis ◽

Coronary Artery Stenosis ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Low Density ◽

Gradient Gel Electrophoresis ◽

Lipoprotein Particle Size

Download Full-text

LDL Particle Size by Gradient-Gel Electrophoresis Cannot Be Estimated by LDL-Cholesterol/Apolipoprotein B Ratios

Clinical Chemistry ◽

10.1093/clinchem/46.8.1202 ◽

2000 ◽

Vol 46 (8) ◽

pp. 1202-1203 ◽

Cited By ~ 10

Author(s):

Daisuke Furuya ◽

Atsuhito Yagihashi ◽

Syunichi Nasu ◽

Teruo Endoh ◽

Tohru Nakamura ◽

...

Keyword(s):

Particle Size ◽

Apolipoprotein B ◽

Gel Electrophoresis ◽

Ldl Cholesterol ◽

Ldl Particle Size ◽

Gradient Gel Electrophoresis

Download Full-text

Allelopathic and Medicinal plant. 26. Millettia speciosa

Allelopathy Journal ◽

10.26651/allelo.j/2020-51-2-1295 ◽

2020 ◽

Vol 51 (2) ◽

pp. 125-146

Author(s):

Nasiruddin Nasiruddin ◽

Yu Zhangxin ◽

Ting Zhao Chen Guangying ◽

Minghui Ji

Keyword(s):

Community Structure ◽

Medicinal Plant ◽

Gel Electrophoresis ◽

Denaturing Gradient Gel Electrophoresis ◽

Wiener Index ◽

Pseudomonas Spp ◽

Evenness Index ◽

Gradient Gel Electrophoresis ◽

Rhizosphere Pseudomonas

We grew cucumber in pots in greenhouse for 9-successive cropping cycles and analyzed the rhizosphere Pseudomonas spp. community structure and abundance by PCR-denaturing gradient gel electrophoresis and quantitative PCR. Results showed that continuous monocropping changed the cucumber rhizosphere Pseudomonas spp. community. The number of DGGE bands, Shannon-Wiener index and Evenness index decreased during the 3rd cropping and thereafter, increased up to the 7th cropping, however, however, afterwards they decreased again. The abundance of Pseudomonas spp. increased up to the 5th successive cropping and then decreased gradually. These findings indicated that the structure and abundance of Pseudomonas spp. community changed with long-term cucumber monocropping, which might be linked to soil sickness caused by its continuous monocropping.

Download Full-text