Regulation of adipocyte gene expression by retinoic acid and hormones: effects on the gene encoding cellular retinol-binding protein

Cellular retinol-binding protein type II (CRBPII) is involved in the transport of vitamin A and its metabolism in the small intestine. In the present study, we demonstrated diet-related variations in CRBPII expression in rat jejunum. The CRBPII protein and mRNA levels increased in parallel after the start of feeding period regardless of whether the feeding period was restricted to the hours of darkness or of light. In addition, this variation was observed in the rats fed high-fat diet or low-fat diets, but not in those fed a fat-free diet or in fasted rats. A similar diet-induced variation was seen in the mRNA of liver-type fatty acid-binding protein in rat jejunum. In the transient transfection experiment, unsaturated fatty acid increased rat CRBPII gene promoter activity via the PPARα/retinoid X receptor-α heterodimer. Taken together, these results suggest that the diet-related variation in CRBPII expression in rat jejunum may be brought about by the transcriptional induction of CRBPII gene expression mainly triggered by dietary fatty acids.

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Mutations in the gene encoding retinol binding protein and retinol deficiency: is there compensation by retinyl esters and retinoic acid?

American Journal of Clinical Nutrition ◽

10.1093/ajcn/69.5.829 ◽

1999 ◽

Vol 69 (5) ◽

pp. 829-830 ◽

Cited By ~ 4

Author(s):

A Catharine Ross

Keyword(s):

Retinoic Acid ◽

Binding Protein ◽

Retinol Binding Protein ◽

Gene Encoding ◽

Retinol Deficiency ◽

Retinyl Esters

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Cellular retinol binding protein 1 transfection reduces proliferation and AKT-related gene expression in H460 non-small lung cancer cells

Molecular Biology Reports ◽

10.1007/s11033-020-05744-5 ◽

2020 ◽

Vol 47 (9) ◽

pp. 6879-6886

Author(s):

Amedeo Ferlosio ◽

Elena Doldo ◽

Sara Agostinelli ◽

Gaetana Costanza ◽

Federica Centofanti ◽

...

Keyword(s):

Gene Expression ◽

Lung Cancer ◽

Binding Protein ◽

Retinol Binding Protein ◽

Nsclc Cell Line ◽

Related Gene ◽

Down Regulation ◽

Mammalian Expression ◽

H460 Cells ◽

Cellular Retinol Binding Protein

Abstract In recent years, new treatments with novel action mechanisms have been explored for advanced non-small cell lung cancer (NSCLC). Retinoids promote cancer cell differentiation and death and their trafficking and action is mediated from specific cytoplasmic and nuclear receptors, respectively. The purpose of this study was to investigate the effect of Cellular retinol binding protein-1 (CRBP-1) transfection in H460 human NSCLC cell line, normally not expressing CRBP-1. H460 cells were transfected by using a vector pTargeT Mammalian expression system carrying the whole sequence of CRBP-1 gene. For proliferation and apoptosis studies, cells were treated with different concentrations of all-trans Retinoic Acid (atRA) and retinol. AKT-related gene expression was analyzed by using western blot and Signosis array and results analysed by one-way analysis of variance (ANOVA) or by t-student test. CRBP-1+ showed reduced proliferation and viability in basal condition and after atRA treatment when compared to empty-transfected H460 cells. Reduced proliferation in CRBP-1+ H460 cells associated to the down-regulation of pAKT/pERK/pEGFR-related genes. In particular, gene array documented the down-regulation of AKT and Stat-3-related genes, including M-Tor, Akt1, Akt2, Akt3, Foxo1, p27, Jun. Restoration of CRBP-1 expression in H460 cells reduced proliferation and viability in both basal condition and after atRA treatment, likely by down-regulating AKT-related gene level. Further studies are needed to better clarify how those CRBP-1-related intracellular pathways contribute to counteract NSCLC progression in order to suggest a potential tool to improve efficacy of retinoid anti lung cancer adjuvant therapy.

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Analysis of human cellular retinol-binding protein II promoter during enterocyte differentiation

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.00041.2001 ◽

2002 ◽

Vol 282 (6) ◽

pp. G1079-G1087 ◽

Cited By ~ 10

Author(s):

Liang Zhang ◽

Xueping E ◽

Kathryn E. Luker ◽

Jian-Su Shao ◽

Marc S. Levin ◽

...

Keyword(s):

Retinoic Acid ◽

Binding Protein ◽

Retinol Binding Protein ◽

Direct Sequence Analysis ◽

Selective Agonists ◽

Flanking Region ◽

Small Intestinal ◽

Small Intestinal Villus ◽

Enterocyte Differentiation ◽

Cellular Retinol Binding Protein

Cellular retinol binding protein II (CRBP II) is a vitamin A-binding protein that is expressed specifically in small intestinal villus absorptive cells. Previous studies have shown that retinoic acid upregulates endogenous human CRBP II gene expression in differentiated Caco-2 cells. To better characterize the regulation of human CRBP II expression, we analyzed the ability of receptor-selective agonists to enhance transcription from the 5′-upstream flanking region of the human CRBP II gene. Stable transfection experiments showed that the proximal 2.8-kb region of the human CRBP II gene is sufficient for retinoic acid inducibility in differentiated Caco-2 cells. However, direct sequence analysis and transient transfection experiments indicate that, unlike the rat CRBP II promoter, the human CRBP II promoter is not a direct retinoid X receptor target. The results indicate that the retinoic acid responsiveness of the human CRBP II promoter is mediated by an indirect mechanism and that this mechanism is associated with enterocyte differentiation.

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