scholarly journals Binding of long-chain fatty acids to bovine serum albumin

1969 ◽  
Vol 10 (1) ◽  
pp. 56-67
Author(s):  
Arthur A. Spector ◽  
Kathryn John ◽  
John E. Fletcher
Keyword(s):  
Fatty Acids ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Long Chain Fatty Acids ◽  
Long Chain ◽  
Chain Fatty Acids

scholarly journals CELLULAR ENERGY METABOLISM DURING FETAL DEVELOPMENT

1970 ◽  
Vol 44 (2) ◽  
pp. 354-360 ◽  
Author(s):  
Joseph B. Warshaw ◽  
Mary L. Terry
Keyword(s):  
Fatty Acids ◽  
Bovine Serum Albumin ◽  
Oxidative Phosphorylation ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Fetal Heart ◽  
Heart Mitochondria ◽  
Long Chain Fatty Acids ◽  
Long Chain ◽  
Chain Fatty Acids

In view of the importance of fatty acids as substrates for the mature heart, fatty acid oxidation by fetal and calf heart mitochondria has been investigated. Free fatty acids of 10 carbon units or less which exhibit carnitine-independent transport into mitochondria were effective substrates for oxidative phosphorylation in both fetal and calf heart mitochondria. Efficient oxidative phosphorylation with these substrates was dependent upon the presence of bovine serum albumin in the assay medium to reverse the uncoupling effects of the fatty acids. In the presence of bovine serum albumin, ADP/0 ratios were in the range of 3 when short-chain fatty acids and carnitine esters of short- and long-chain fatty acids were substrates. Compared with calf heart mitochondria, fetal heart mitochondria showed decreased carnitine-dependent oxidation of palmityl-CoA. However, the oxidation of palmitylcarnitine was identical in both. These data suggest that the formation of palmitylcarnitine is rate limiting for palmityl-CoA oxidation by the fetal heart mitochondria and that long-chain fatty acids are not readily oxidized by the fetal heart.

scholarly journals Long chain fatty acids alter the interactive binding of ligands to the two principal drug binding sites of human serum albumin

PLoS ONE ◽  
2017 ◽  
Vol 12 (6) ◽  
pp. e0180404 ◽  
Author(s):  
Keishi Yamasaki ◽  
Saya Hyodo ◽  
Kazuaki Taguchi ◽  
Koji Nishi ◽  
Noriyuki Yamaotsu ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Binding Sites ◽  
Drug Binding ◽  
Long Chain Fatty Acids ◽  
Drug Binding Sites ◽  
Long Chain ◽  
Chain Fatty Acids

scholarly journals Binding of long-chain fatty acids to serum albumin in healthy humans Relationship to obesity

1991 ◽  
Vol 197 (2) ◽  
pp. 461-465 ◽  
Author(s):  
Rolf BRODERSEN ◽  
Henrik VORUM ◽  
Niels KRUKOW ◽  
Anders Overgaard PEDERSEN
Keyword(s):  
Fatty Acids ◽  
Serum Albumin ◽  
Long Chain Fatty Acids ◽  
Healthy Humans ◽  
Long Chain ◽  
Chain Fatty Acids

scholarly journals THE EFFECT OF LIPIDS AND SERUM ALBUMIN ON BACTERIAL GROWTH

1947 ◽  
Vol 85 (1) ◽  
pp. 9-22 ◽  
Author(s):  
René J. Dubos
Keyword(s):  
Fatty Acids ◽  
Serum Albumin ◽  
Culture Medium ◽  
Unsaturated Fatty Acids ◽  
Sole Source ◽  
Water Soluble ◽  
Long Chain Fatty Acids ◽  
Microbial Species ◽  
Long Chain ◽  
Chain Fatty Acids

Long chain fatty acids have been found to exhibit both inhibitory and stimulatory effects on the growth of tubercle bacilli and of a certain unidentified micrococcus culture. The toxicity of the fatty acids was much reduced or abolished by (a) esterification, even when the resulting product was a water-soluble ester, and (b) addition of crystalline serum albumin to the culture medium; other proteins tested were inactive in this respect. Marked growth stimulation of the microorganisms studied was obtained when certain long chain fatty acids were added to the culture medium in the form of their water-soluble esters, or in admixture with adequate amounts of serum albumin. Abundant growth of the micrococcus resulted from the addition of oleic, linoleic, linolenic, or arachidonic acid (0.0001 to 0.001 per cent) to a mineral medium containing glucose as sole source of carbon; in the case of this microbial species, none of the other substances tested could substitute for these unsaturated fatty acids. Enhancement of growth of tubercle bacilli was obtained by adding to the medium 0.001 to 0.01 per cent of a variety of fatty acids (saturated or unsaturated) even in the absence of glucose or of any other readily available carbon compound. These results suggest that long chain fatty acids can affect the growth of different microbial species through different metabolic channels and that, in order to study the mechanism of these metabolic and growth reactions, it is essential to use the fatty acids under conditions where they cannot manifest their toxic properties.

Sign in / Sign up

Export Citation Format

Share Document