The Role of Bacterial Virulence Factors and Tamm-Horsfall Protein in the Pathogenesis of Escherichia Coli Urinary Tract Infection in Infants

1988 ◽  
Vol 139 (3) ◽  
pp. 657-658
Author(s):  
V. Israele ◽  
A. Darabi ◽  
G.H. McCracken
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Virulence Factors ◽  
Bacterial Virulence

scholarly journals Uropathogenic Escherichia coli-Induced Inflammation Alters Mouse Urinary Bladder Contraction via an Interleukin-6-Activated Inducible Nitric Oxide Synthase-Related Pathway

2009 ◽  
Vol 77 (8) ◽  
pp. 3312-3319 ◽  
Author(s):  
Te I. Weng ◽  
Hsiao Yi Wu ◽  
Pei Ying Lin ◽  
Shing Hwa Liu
Keyword(s):  
Nitric Oxide ◽  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Urinary Bladder ◽  
Tract Infection ◽  
Interleukin 6 ◽  
Electrical Field Stimulation ◽  
E Coli

ABSTRACT Escherichia coli is the most common cause of urinary tract infection. Elevated blood and urine interleukin-6 (IL-6) levels have been shown in inflammatory urinary tract diseases. The role of IL-6 in mediating the urodynamic dysfunction in response to E. coli-induced urinary tract infection has not yet been fully elucidated. In this study, we investigated the role of IL-6 in the nitric oxide (NO)-triggered alteration of contractile responses in the urinary bladder under an E. coli-induced inflammatory condition. The electrical field stimulation (EFS)-evoked contractions of the isolated detrusor strips, and immunoblotting for detecting protein expression in the bladders was measured short term (1 h) or long term (6 or 24 h) after intraperitoneal injection of E. coli endotoxin (lipopolysaccharide [LPS]) or intravesical instillation of human pyelonephritogenic E. coli-J96 (O4:K6) strain or LPS into mice. IL-6 and NO productions were increased in the urinary bladders of mice 1 to 24 h after LPS or E. coli-J96 treatment. Inducible NO synthase (iNOS) expression and protein kinase C (PKC) activation and EFS-evoked detrusor contractions were increased in the bladders at 6 h after LPS or E. coli-J96 treatment, which could be reversed by anti-IL-6 antibody and iNOS inhibitor aminoguanidine. At 1 h after LPS administration, bladder NO generation, endothelial NOS expression, and EFS-evoked detrusor contractions were effectively increased, whereas anti-IL-6 antibody could not reverse these LPS-induced responses. These results indicate that IL-6 may play an important role in the iNOS/NO-triggered PKC-activated contractile response in urinary bladder during E. coli or LPS-induced inflammation.

scholarly journals Escherichia coli Isolates That Carryvat,fyuA,chuA, andyfcVEfficiently Colonize the Urinary Tract

2012 ◽  
Vol 80 (12) ◽  
pp. 4115-4122 ◽  
Author(s):  
Rachel R. Spurbeck ◽  
Paul C. Dinh ◽  
Seth T. Walk ◽  
Ann E. Stapleton ◽  
Thomas M. Hooton ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Virulence Factors ◽  
Neonatal Meningitis ◽  
Content Type ◽  
E Coli ◽  
Core Set ◽  
Encoding Genes

ABSTRACTExtraintestinalEscherichia coli(ExPEC), a heterogeneous group of pathogens, encompasses avian, neonatal meningitis, and uropathogenicE. colistrains. While several virulence factors are associated with ExPEC, there is no core set of virulence factors that can be used to definitively differentiate these pathotypes. Here we describe a multiplex of four virulence factor-encoding genes,yfcV,vat,fyuA, andchuA, highly associated with uropathogenicE. colistrains that can distinguish three groups ofE. coli: diarrheagenic and animal-associatedE. colistrains, human commensal and avian pathogenicE. colistrains, and uropathogenic and neonatal meningitisE. colistrains. Furthermore, human intestinal isolates that encode all four predictor genes express them during exponential growth in human urine and colonize the bladder in the mouse model of ascending urinary tract infection in higher numbers than human commensal strains that do not encode the four predictor genes (P= 0.02), suggesting that the presence of the predictors correlates with uropathogenic potential.

scholarly journals Virulence factors, antibiotic resistance phenotypes and O-serogroups of Escherichia coli strains isolated from community-acquired urinary tract infection patients in Mexico

2017 ◽  
Vol 50 (4) ◽  
pp. 478-485 ◽  
Author(s):  
Gloria Luz Paniagua-Contreras ◽  
Eric Monroy-Pérez ◽  
José Raymundo Rodríguez-Moctezuma ◽  
Pablo Domínguez-Trejo ◽  
Felipe Vaca-Paniagua ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Antibiotic Resistance ◽  
Urinary Tract ◽  
Tract Infection ◽  
Virulence Factors

The Role of Bacterial Virulence and Host Factors in Patients withEscherichia coliBacteremia Who Have Acute Cholangitis or Upper Urinary Tract Infection

2002 ◽  
Vol 35 (10) ◽  
pp. 1161-1166 ◽  
Author(s):  
Ming‐Cheng Wang ◽  
Chin‐Chung Tseng ◽  
Chiung‐Yu Chen ◽  
Jiunn‐Jong Wu ◽  
Jeng‐Jong Huang
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Acute Cholangitis ◽  
Upper Urinary Tract ◽  
Bacterial Virulence ◽  
Host Factors

scholarly journals Virulence genes and phylogenetic groups of uropathogenic Escherichia coli isolates from patients with urinary tract infection and uninfected control subjects: a case-control study

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Seyedeh Elham Rezatofighi ◽  
Mahsa Mirzarazi ◽  
Mansour Salehi
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Virulence Factors ◽  
Virulence Genes ◽  
Strong Association ◽  
Phylogenetic Groups ◽  
E Coli ◽  
Healthy Humans

Abstract Background Urinary Tract Infection (UTI) is one of the most common bacterial infectious diseases which causes considerable morbidity and costly health problems. Uropathogenic Escherichia coli (UPEC), the most common pathogen causing UTI, is a highly heterogeneous group of extraintestinal pathogenic E. coli (ExPEC) which may carry a variety of virulence factors and belonging to different phylogenetic backgrounds. The current study aimed to investigate the frequency and association between various virulence factors (VFs) and phylogenetic groups of UPEC and commensal isolates. Methods UPEC and commensal E. coli strains isolated from UTI and feces of healthy humans were compared for the presence of VFs and phylogenetic groups. Association between virulence genes was investigated and cluster analysis was employed. Results According to the results, among a 30 virulence markers tested, the pathogenicity-associated island (PAI), papAH, papEF, fimH, fyuA, and traT genes prevalence were statistically significant in UPEC isolates. A strong association was found between the B2 and D phylogenetic groups and clinical isolates of UPEC; while, commensal isolates were mostly associated with phylogenetic group A. The aggregated VFs scores were more than twice higher in the UPEC isolates in comparison with the commensal isolates. Interestingly, the B2 group in both UPEC and commensal isolates had the highest VF scores. A strong positive association was found between several virulence genes. The clustering results demonstrated that UPEC or commensal E. coli isolates were highly heterogeneous due to different composition of their virulence gene pool and pathogenicity islands. Conclusion Genetic structure and VFs of UPEC strains vary from region to region; therefore, to control the UTI, the epidemiological aspects and characterization of the UPEC isolates need to be investigated in different regions. Since UPEC isolates are generally originate from the commensal strains, it may be feasible to reduce the UTI burden by interfering the intestinal colonization, particularly in the highly pathogenic clonal lineages such as B2.

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