P XIV C.9 Molecular characterization of micronuclei in peripheral blood reticulocytes of the mouse by means of an in situ RT-PCR/PRINS approach

1997 ◽  
Vol 379 (1) ◽  
pp. S137
Author(s):  
Antonella Russo ◽  
Cristiano Salata
Keyword(s):  
Molecular Characterization ◽  
Peripheral Blood ◽  
Rt Pcr

scholarly journals The identification and characterization of a testis-specific cDNA during spermatogenesis

2006 ◽  
Vol 11 (1) ◽  
Author(s):  
Ying Chen ◽  
Jiarui Hu ◽  
Ping Song ◽  
Wuming Gong
Keyword(s):  
Experimental Validation ◽  
Rna Binding ◽  
Pcr Analysis ◽  
Splicing Factors ◽  
Rt Pcr ◽  
Rich Domain ◽  
Pachytene Spermatocytes ◽  
Identification And Characterization

AbstractUsing bioinformatics and experimental validation, we obtained a cDNA (named srsf) which was exclusively expressed in the mouse testes. RT-PCR analysis showed that srsf mRNA was not expressed in the gonad during the sex determination period or during embryogenesis. In developing mouse tests, srsf expression was first detected on post-natal day 10, reached its highest level on day 23, and then reduced to and remained at a moderate level throughout adulthood. In situ hybridization analysis demonstrated that srsf mRNA was expressed in pachytene spermatocytes and round spermatids in the testes. The predicted protein contains one RNA-binding domain (RBD) and a serine-arginine rich domain (RS), which are characterized by some splicing factors of SR family members. These findings indicate that srsf may play a role during spermatogenesis.

Validation of RT-PCR Assays for Molecular Characterization of Porcine Teschoviruses and Enteroviruses

2006 ◽  
Vol 53 (6) ◽  
pp. 257-265 ◽  
Author(s):  
G. La Rosa ◽  
M. Muscillo ◽  
A. Di Grazia ◽  
S. Fontana ◽  
M. Iaconelli ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Rt Pcr ◽  
Pcr Assays

scholarly journals Molecular characterization of two rocio flavivirus strains isolated during the encephalitis epidemic in são paulo state, brazil and the development of a one-step rt-pcr assay for diagnosis

2008 ◽  
Vol 50 (2) ◽  
pp. 89-94 ◽  
Author(s):  
Terezinha Lisieux Moraes Coimbra ◽  
Raimundo N. Santos ◽  
Selma Petrella ◽  
Teresa Keico Nagasse-Sugahara ◽  
Silvana Beres Castrignano ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Clinical Manifestations ◽  
Cross Reactivity ◽  
Sao Paulo ◽  
São Paulo ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Paulo State ◽  
São Paulo State

Rocio virus (ROCV) was responsible for an explosive encephalitis epidemic in the 1970s affecting about 1,000 residents of 20 coastland counties in São Paulo State, Brazil. ROCV was first isolated in 1975 from the cerebellum of a fatal human case of encephalitis. Clinical manifestations of the illness are similar to those described for St. Louis encephalitis. ROCV shows intense antigenic cross-reactivity with Japanese encephalitis complex (JEC) viruses, particularly with Ilheus (ILHV), St. Louis encephalitis, Murray Valley and West Nile viruses. In this study, we report a specific RT-PCR assay for ROCV diagnosis and the molecular characterization of the SPAn37630 and SPH37623 strains. Partial nucleotide sequences of NS5 and E genes determined from both strains were used in phylogenetic analysis. The results indicated that these strains are closely related to JEC viruses, but forming a distinct subclade together with ILHV, in accordance with results recently reported by Medeiros et al. (2007).

scholarly journals RT-PCR and CP gene based molecular characterization of a cucumber mosaic cucumovirus from Aligarh, U.P., India

2012 ◽  
Vol 03 (08) ◽  
pp. 971-978 ◽  
Author(s):  
Shahid Ali ◽  
Masood Akhtar ◽  
Kangabam S. Singh ◽  
Qamar A. Naqvi
Keyword(s):  
Molecular Characterization ◽  
Rt Pcr ◽  
Cp Gene ◽  
Cucumber Mosaic Cucumovirus

scholarly journals Identification and molecular characterization of onion yellow dwarf virus (OYDV) in Allium cepa crops in Poland

2021 ◽  
Vol 61 (3) ◽  
pp. 214-220
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Characterization ◽  
Coat Protein Sequence ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Onion Yellow Dwarf Virus ◽  
Rt Pcr ◽  
Pcr Products ◽  
Yellow Dwarf Virus

Onion yellow dwarf virus is distributed worldwide significantly reducing yield of crops from the Allium genus. The aim of the study was the detection and molecular characterization of newly identified OYDV isolates infecting onions in Poland. The virus was detected by transmission electron microscopy and RT-PCR techniques using two pairs of diagnostic primers: OYDV-NibCPF1/R1 and OYDV-CPF2/R2. The specificity of obtained RT-PCR products was confirmed by Sanger sequencing and received viral coat protein sequence was used for phylogenetic analysis. The phylogenetic analysis was carried out using CP sequences of the new Polish onion isolate obtained in this study and 37 other sequences of OYDV retrieved from GenBank. The analysis revealed that the Polish OYDV isolate is the most similar to the OYDV isolates derived from onions from Argentina and Germany, which may indicate their common origin. Moreover, it was observed that the Polish onion and garlic isolates are very diverse and belong to different phylogroups.

296. Characterization of E74 like factor 3 in the murine pre-implantation embryo

2005 ◽  
Vol 17 (9) ◽  
pp. 125
Author(s):  
E. B. Little ◽  
J. L. Stanton ◽  
D. P. L. Green
Keyword(s):  
In Situ Hybridisation ◽  
Specific Gene ◽  
Hydrophobic Core ◽  
Rt Pcr ◽  
Core Motif ◽  
Cell Embryo ◽  
Ets Family ◽  
Ets Factors

The E26 transformation specific (ETS) family of transcription factors is defined by a highly conserved DNA binding domain containing a hydrophobic core motif 5′-GGA[A/T]-3′. A subfamily of ETS factors, epithelium-specific ETS factor (ESE), includes E74-like factor 3 (Elf3). Elf3 is expressed in many organs including the eye, skin and gastrointestinal tract. By upregulating specific gene transcription, Elf3 is instrumental in causing these organs to become fully differentiated. The presence of Elf3 in the pre-implantation embryo was first reported in 2002.1 Data mining has suggested that the expression of Elf3 increases at the 16-cell embryo and blastocyst stages of pre-implantation development compared to the preceding stages. Reverse transcriptase–polymerase chain reaction (RT-PCR) was used to amplify the 3′ untranslated region (3′UTR) of Elf3. These results suggest that there are two isoforms of Elf3 present in the pre-implantation embryo, which differ by an 88 bp deletion/insertion. The expression and location of these isoforms was investigated using RT-PCR and in-situ hybridisation. (1)Stanton and Green (2002) Mol. Hum. Rep. 8, 149–166.

Sign in / Sign up

Export Citation Format

Share Document